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[180009_at]

Description F59A6.5 /REP_DB=WormBase Gene ID /WP=CE02790 /TR=Q21025 /GB=AAK31526.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_high_8_8 > 51      6grp_highest_nonorm > 93      6grp_total > 68      4grp_total > 52      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 246
1 2 3 4 range
A 303.5
(3)
P 		269.7
(1.7)
P 		259
(1.3)
P 		146.5
(-3)
A 		157
B 269.2
(1.4)
P 		331.8
(3)
P 		209.4
(-0.1)
P 		94.1
(-3)
P 		238
C 203.1
(0.2)
P 		305.9
(3)
P 		258
(1.7)
P 		85.8
(-3)
P 		220
avr 258.6
(1.6) 		302.5
(3) 		242.1
(1.1) 		108.8
(-3) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 501
1 2 3 4 5 6 range
A 292.6
(-1.8)
P 		303.3
(-1.6)
P 		189.6
(-3)
P 		245.9
(-2.3)
P 		333.6
(-1.3)
P 		687.3
(3)
P 		498
B 278.3
(-2.1)
P 		264.9
(-2.3)
P 		206.7
(-3)
P 		266.9
(-2.3)
P 		364.7
(-1)
P 		690.7
(3)
P 		484
C 300.6
(-1.8)
P 		248.3
(-2.4)
P 		204.7
(-3)
P 		228.4
(-2.7)
P 		328.2
(-1.4)
P 		665.9
(3)
P 		461
avr 290.5
(-1.9) 		272.2
(-2.1) 		200.3
(-3) 		247.1
(-2.4) 		342.2
(-1.2) 		681.3
(3) 		481
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 110.5
(3)
P 		58.5
(-0.5)
P 		67.7
(0.1)
P 		21.4
(-3)
A 		89
B 85.8
(3)
P 		81.9
(2.6)
P 		48.5
(-0.4)
P 		20.1
(-3)
P 		66
C 67.2
(1.2)
P 		88
(2.8)
P 		89.9
(3)
P 		14.5
(-3)
P 		75
avr 87.8
(3) 		76.1
(2) 		68.7
(1.3) 		18.7
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 216
1 2 3 4 5 6 range
A 53.9
(-2.4)
P 		107.9
(0.2)
P 		41.3
(-3)
P 		62.4
(-2)
P 		100.7
(-0.2)
P 		167.3
(3)
P 		126
B 96.8
(-1.7)
P 		88.2
(-1.9)
P 		50.2
(-3)
P 		70.7
(-2.4)
P 		148.2
(-0.2)
P 		257.6
(3)
P 		207
C 111.4
(-0.8)
P 		75.8
(-2)
P 		52.1
(-2.8)
P 		45.9
(-3)
P 		106.2
(-1)
P 		222.9
(3)
P 		177
avr 87.4
(-1.6) 		90.6
(-1.5) 		47.9
(-3) 		59.7
(-2.6) 		118.4
(-0.5) 		215.9
(3) 		168
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180009_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4137
Sequence Type Exemplar sequence
Representative Public ID F59A6.5 NCBI
Target Description F59A6.5 /REP_DB=WormBase Gene ID /WP=CE02790 /TR=Q21025 /GB=AAK31526.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062520(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062520 NCBI Caenorhabditis elegans F59A6.5 (F59A6.5) mRNA, complete cds. 11/11 None
SNAP00000007645 ENSEMBL cdna:SNAP chromosome:CEL140:II:4999509:5003626:1 11/11 None
GENEFINDER00000007654 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4999509:5003626:1 11/11 None
F59A6.5 ENSEMBL cdna:known chromosome:CEL140:II:4999509:5003778:1 gene:F59A6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4999506-5003624(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F59A6.5
Entrez Gene 173864 Entrez gene
SwissProt Q21025 EMBL-EBI
WormBase CE02790 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5083 small GTPase regulator activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31526 Hypothetical protein F59A6.5 [Caenorhabditis elegans] ref|NP_494921.1| F59A6.5 [Caenorhabditis elegans] 0.0
blast CAE61028 Hypothetical protein CBG04771 [Caenorhabditis briggsae] 0.0
blast CAB03458 Hypothetical protein W02B8.2 [Caenorhabditis elegans] ref|NP_496914.1| W02B8.2 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001180 EMBL-EBI Citron-like 1.7E-90
Pfam IPR001849 EMBL-EBI Pleckstrin-like 4.7E-13
Sequence
Target Sequence 
>C. ELEGANS:180009_AT
tgcgaaattcgacgttcctgaaccaataagcttcatcgaaagtgctccatcgggaatcat
cttcgcatgtgacacattctattatgtcgccttggatcaccaaacttcgtccaacgtttc
cgctcgaaaattgatgtctccaaataaaaacgaattcccgatcagtgctcagatgataaa
tcaaaatgaagtgctcctggcttatcaaaaccagggaatttttgtgaatttgcacggaga
acaatcacgaaacgaaacaattgaatgggagaagatgccgatggagttcatctacacatc
accattcctgtatattgttcacgatgactcgatcgaaattctggaaatttctgaaacctc
ggataggactgtgttggctgagcgagccctttttgagtgtgtcaatgctcatgtgattgg
acgccaatatgaaggcgtactgatttccgtttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGAAATTCGACGTTCCTGAACCA 472 583 3300 Antisense
GCTTCATCGAAAGTGCTCCATCGGG 300 305 3329 Antisense
CTCCATCGGGAATCATCTTCGCATG 79 235 3344 Antisense
CTATTATGTCGCCTTGGATCACCAA 512 223 3378 Antisense
GTCCAACGTTTCCGCTCGAAAATTG 454 477 3408 Antisense
CGAATTCCCGATCAGTGCTCAGATG 638 251 3450 Antisense
GAAGTGCTCCTGGCTTATCAAAACC 631 335 3487 Antisense
CATCTACACATCACCATTCCTGTAT 368 213 3588 Antisense
ATAGGACTGTGTTGGCTGAGCGAGC 379 19 3662 Antisense
AGCGAGCCCTTTTTGAGTGTGTCAA 683 81 3680 Antisense
TGAAGGCGTACTGATTTCCGTTTCG 37 575 3729 Antisense
Sequence Source Affymetrix Proprietary Database