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[180000_at]

Description C27F2.1 /REP_DB=WormBase Gene ID /WP=CE26874 /TR=Q18263 /GB=AAA81428.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 56
(3)
P 		23
(-2.6)
A 		39.8
(0.2)
A 		20.7
(-3)
A 		35
B 36.4
(0.6)
A 		29.1
(-0.2)
A 		3.3
(-3)
A 		57.8
(3)
A 		55
C 42.3
(-2.4)
A 		34.6
(-2.9)
A 		32.5
(-3)
A 		130.5
(3)
A 		98
avr 44.9
(-0.3) 		28.9
(-2.5) 		25.2
(-3) 		69.7
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 62.1
(3)
A 		9.5
(-3)
A 		19.6
(-1.8)
A 		40.5
(0.5)
A 		44.9
(1)
A 		11.4
(-2.8)
A 		53
B 46.1
(3)
A 		14.2
(-2.8)
A 		23.1
(-1.2)
A 		16.3
(-2.4)
A 		13.2
(-3)
A 		13.4
(-3)
A 		33
C 18.4
(-2.2)
A 		59.9
(1.8)
A 		26.9
(-1.4)
A 		72.1
(3)
A 		10.2
(-3)
A 		43.4
(0.2)
A 		62
avr 42.2
(2.8) 		27.9
(-1.5) 		23.2
(-2.9) 		43
(3) 		22.8
(-3) 		22.7
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 20.4
(3)
P 		5
(-2.3)
A 		10.4
(-0.4)
A 		3
(-3)
A 		17
B 11.6
(2.6)
A 		7.2
(0.3)
A 		0.8
(-3)
A 		12.3
(3)
A 		12
C 14
(-1)
A 		9.9
(-3)
A 		11.3
(-2.3)
A 		22
(3)
A 		12
avr 15.3
(3) 		7.4
(-3) 		7.5
(-2.9) 		12.4
(0.8) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 11.4
(1.8)
A 		3.4
(-2.7)
A 		4.3
(-2.2)
A 		10.3
(1.2)
A 		13.6
(3)
A 		2.8
(-3)
A 		11
B 16
(3)
A 		4.7
(-2.8)
A 		5.6
(-2.3)
A 		4.3
(-3)
A 		5.4
(-2.4)
A 		5
(-2.6)
A 		12
C 6.8
(-1.6)
A 		18.3
(3)
A 		6.8
(-1.6)
A 		14.5
(1.5)
A 		3.3
(-3)
A 		14.5
(1.5)
A 		15
avr 11.4
(3) 		8.8
(0.3) 		5.6
(-3) 		9.7
(1.2) 		7.4
(-1.1) 		7.4
(-1.1) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180000_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7233
Sequence Type Exemplar sequence
Representative Public ID C27F2.1 NCBI
Target Description C27F2.1 /REP_DB=WormBase Gene ID /WP=CE26874 /TR=Q18263 /GB=AAA81428.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065657(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065657 NCBI Caenorhabditis elegans C27F2.1 (C27F2.1) mRNA, complete cds. 11/11 A 
GENEFINDER00000001023 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4987230:4991357:1 11/11 None
C27F2.1 ENSEMBL cdna:known chromosome:CEL140:III:4987230:4991357:1 gene:C27F2.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4987228-4991356(+) 89.04 100.0
Public Domain and Genome References
Gene Title
Ensembl C27F2.1
Entrez Gene 175676 Entrez gene
WormBase CE38940 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498058 C27F2.1 [Caenorhabditis elegans] 0.0
blast AAA81428 Hypothetical protein C27F2.1 [Caenorhabditis elegans] 0.0
blast CAE70090 Hypothetical protein CBG16532 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:180000_AT
aaatgaacttcgacttcttctgaccgcagttattcgccctcatcctgtcctaaagaggtc
acgtggtgtcagaatgtctcttttcggtttgatctccgaaaaatgcgggagaaaagacgc
agagttttcaactgattttgcatggttaaggacgtggatacgtcacaattttttggccaa
aaaaattcccgcatttttcgtagatcaaaccgcaatgagacatcctgacaccacacattc
tacatcattcgccgtctccgctttctgccacgtcaccaacccgttgagatttttcatcgg
aaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGAACTTCGACTTCTTCTGACC 658 119 1386 Antisense
ACTTCTTCTGACCGCAGTTATTCGC 441 103 1398 Antisense
CGCCCTCATCCTGTCCTAAAGAGGT 283 255 1420 Antisense
GGTCACGTGGTGTCAGAATGTCTCT 696 503 1442 Antisense
AATGTCTCTTTTCGGTTTGATCTCC 31 165 1458 Antisense
GGATACGTCACAATTTTTTGGCCAA 179 509 1541 Antisense
CATTTTTCGTAGATCAAACCGCAAT 157 215 1577 Antisense
AACCGCAATGAGACATCCTGACACC 120 147 1593 Antisense
TGACACCACACATTCTACATCATTC 18 573 1611 Antisense
CCACGTCACCAACCCGTTGAGATTT 83 275 1653 Antisense
CCCGTTGAGATTTTTCATCGGAACA 638 259 1665 Antisense
Sequence Source Affymetrix Proprietary Database