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[179998_at]

Description F20B4.4 /REP_DB=WormBase Gene ID /WP=CE07103 /TR=Q19621 /GB=AAC48145.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 08      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 7.4
(-3)
A 		12.1
(0.4)
A 		15.8
(3)
A 		15
(2.4)
A 		8
B 9.9
(-0.6)
A 		9.4
(-0.9)
A 		5.5
(-3)
A 		16.5
(3)
A 		11
C 15.4
(-1.5)
A 		13.2
(-3)
A 		16.2
(-0.9)
A 		21.9
(3)
A 		9
avr 10.9
(-3) 		11.6
(-2.4) 		12.5
(-1.6) 		17.8
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 7
(-2.4)
A 		10
(-0.9)
A 		5.8
(-3)
A 		10.5
(-0.6)
A 		6.2
(-2.8)
A 		17.6
(3)
A 		12
B 15.9
(1.5)
A 		6.9
(-2.1)
A 		19.6
(3)
A 		4.8
(-3)
A 		9.2
(-1.2)
A 		7.9
(-1.7)
A 		15
C 6.3
(-3)
A 		13
(3)
A 		8.7
(-0.9)
A 		8.5
(-1)
A 		7.1
(-2.3)
A 		11.5
(1.7)
A 		7
avr 9.7
(-0.3) 		10
(0.1) 		11.4
(1.9) 		7.9
(-2.5) 		7.5
(-3) 		12.3
(3) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 2.7
(-1.4)
A 		2.6
(-1.7)
A 		4.1
(3)
A 		2.2
(-3)
A 		2
B 3.1
(1.9)
A 		2.3
(-0.3)
A 		1.3
(-3)
A 		3.5
(3)
A 		2
C 5.1
(1.4)
A 		3.8
(-2.7)
A 		5.6
(3)
A 		3.7
(-3)
A 		2
avr 3.6
(2.3) 		2.9
(-3) 		3.7
(3) 		3.1
(-1.5) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 1.3
(-3)
A 		3.6
(1.6)
A 		1.3
(-3)
A 		2.7
(-0.2)
A 		1.9
(-1.8)
A 		4.3
(3)
A 		3
B 5.5
(3)
A 		2.3
(-1.6)
A 		4.8
(2)
A 		1.3
(-3)
A 		3.7
(0.4)
A 		2.9
(-0.7)
A 		4
C 2.4
(-1.2)
A 		4
(3)
A 		2.2
(-1.7)
A 		1.7
(-3)
A 		2.3
(-1.4)
A 		3.9
(2.7)
A 		2
avr 3.1
(1) 		3.3
(1.7) 		2.8
(-0) 		1.9
(-3) 		2.6
(-0.7) 		3.7
(3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179998_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17673
Sequence Type Exemplar sequence
Representative Public ID F20B4.4 NCBI
Target Description F20B4.4 /REP_DB=WormBase Gene ID /WP=CE07103 /TR=Q19621 /GB=AAC48145.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078459(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078459 NCBI Caenorhabditis elegans F20B4.4 (F20B4.4) mRNA, complete cds. 11/11 A 
GENEFINDER00000007939 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17706788:17707420:-1 11/11 None
F20B4.4 ENSEMBL cdna:known chromosome:CEL140:X:17706788:17707420:-1 gene:F20B4.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000038814 5/11 Cross Hyb Matching Probes None
ZK1025.1 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Public Domain and Genome References
Gene Title
Ensembl F20B4.4
Entrez Gene 184710 Entrez gene
SwissProt Q19621 EMBL-EBI
WormBase CE07103 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48145 Hypothetical protein F20B4.4 [Caenorhabditis elegans] ref|NP_510860.1| F20B4.4 [Caenorhabditis elegans] 1.0E-101
blast CAA92477 Hypothetical protein T05A1.4 [Caenorhabditis elegans] ref|NP_501697.1| T05A1.4 [Caenorhabditis elegans] 1.0E-79
blast NP_503327 R13D11.7 [Caenorhabditis elegans] gb|AAB69947.1| Hypothetical protein R13D11.7 [Caenorhabditis elegans] 2.0E-43
Sequence
Target Sequence 
>C. ELEGANS:179998_AT
ccccgcatgttgacaacccagaggcacccgaggccacccttcaaggtgactcatcggcta
ccgctcaaggtaacccctcaggcaggcaatcaaaccgacgctcgagacgtggtcgcccac
accacttgagatcgccagccgccactatcaagggtaccatcacccgcatcgtcaataaga
cgcgacagtttcatcaggaagcgtctagaacgatgctggaacattttggttgcaacccga
acgtttctcctcccatcgagaacgctatcctccgcaatgcgaatcaagttctcatcaaac
ttcggcattacgcggaggagttggagaacctacgccaattcgtgaacgacaagttccaag
aaccggcactgcgagattcccccaatagggacacctatcacaaagaggtggaagctcatc
ttgcaggactccaacctcaagccctcctcaacgaggcaaaacgtgacatcgtcatgttgg
aaagagagttgtcatctctcggcttaccagtgaccccccatgttcgtcatgacaacgtac
tcactccgtacgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCCGCATGTTGACAACCCAGAGGC 434 259 59 Antisense
TAACCCCTCAGGCAGGCAATCAAAC 5 639 129 Antisense
AAGACGCGACAGTTTCATCAGGAAG 700 153 235 Antisense
GCTGGAACATTTTGGTTGCAACCCG 150 295 273 Antisense
ACGCTATCCTCCGCAATGCGAATCA 609 91 320 Antisense
GAACCTACGCCAATTCGTGAACGAC 292 329 384 Antisense
CAAGAACCGGCACTGCGAGATTCCC 626 185 415 Antisense
GAGATTCCCCCAATAGGGACACCTA 42 329 431 Antisense
GTGGAAGCTCATCTTGCAGGACTCC 267 491 466 Antisense
CCCCCATGTTCGTCATGACAACGTA 566 259 573 Antisense
GACAACGTACTCACTCCGTACGTGC 502 359 589 Antisense
Sequence Source Affymetrix Proprietary Database