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[179971_at]

Description T12C9.3 /REP_DB=WormBase Gene ID /WP=CE26823 /TR=Q22426 /GB=AAK18976.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 38      6grp_cgc > 50      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 106.7
(3)
A 		64.3
(-0.7)
A 		38
(-3)
A 		87.1
(1.3)
A 		69
B 94.2
(3)
A 		73
(-0.8)
A 		79.8
(0.4)
A 		60.9
(-3)
A 		33
C 100.7
(3)
A 		75.4
(-1.9)
A 		69.7
(-3)
A 		75.5
(-1.9)
A 		31
avr 100.5
(3) 		70.9
(-1.7) 		62.5
(-3) 		74.5
(-1.1) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 121.6
(3)
A 		65.3
(0)
A 		61.1
(-0.2)
A 		90
(1.3)
A 		63.8
(-0)
A 		7.7
(-3)
A 		114
B 90.9
(1.7)
A 		101.7
(2.8)
A 		87.4
(1.4)
A 		103.4
(3)
A 		69.1
(-0.4)
A 		43.6
(-3)
A 		60
C 83.3
(0.9)
A 		107.3
(3)
A 		85.3
(1.1)
A 		99.5
(2.3)
A 		74.3
(0.1)
A 		39.3
(-3)
A 		68
avr 98.6
(3) 		91.4
(2.4) 		77.9
(1.2) 		97.6
(2.9) 		69.1
(0.4) 		30.2
(-3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 38.9
(3)
A 		14
(-2.2)
A 		9.9
(-3)
A 		12.7
(-2.4)
A 		29
B 30
(3)
A 		18
(-1.2)
A 		18.5
(-1.1)
A 		13
(-3)
A 		17
C 33.3
(3)
A 		21.7
(-0.4)
A 		24.3
(0.4)
A 		12.7
(-3)
A 		21
avr 34.1
(3) 		17.9
(-1.6) 		17.6
(-1.6) 		12.8
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 22.4
(2.8)
A 		23.2
(3)
A 		13.3
(0.2)
A 		22.8
(2.9)
A 		19.3
(1.9)
A 		1.9
(-3)
A 		21
B 31.6
(2.2)
A 		33.9
(3)
A 		21.2
(-1.3)
A 		27.4
(0.8)
A 		28.1
(1)
A 		16.3
(-3)
A 		18
C 30.9
(2.4)
A 		32.7
(3)
A 		21.7
(-0.4)
A 		20
(-0.9)
A 		24
(0.3)
A 		13.2
(-3)
A 		20
avr 28.3
(2.5) 		29.9
(3) 		18.7
(-0.5) 		23.4
(1) 		23.8
(1.1) 		10.5
(-3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179971_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4694
Sequence Type Exemplar sequence
Representative Public ID T12C9.3 NCBI
Target Description T12C9.3 /REP_DB=WormBase Gene ID /WP=CE26823 /TR=Q22426 /GB=AAK18976.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062385(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062385 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-2) (twk-2) mRNA, complete cds. 11/11 None
SNAP00000005698 ENSEMBL cdna:SNAP chromosome:CEL140:II:4441255:4453141:-1 11/11 None
GENEFINDER00000005703 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4441255:4443770:-1 11/11 None
T12C9.3 ENSEMBL cdna:known chromosome:CEL140:II:4441255:4451691:-1 gene:T12C9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4441253-4455092(-) 91.14 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-2
Ensembl T12C9.3
Entrez Gene 188452 Entrez gene
SwissProt Q22426 EMBL-EBI
WormBase CE36894 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18976 Twik family of potassium channels protein 2 [Caenorhabditis elegans] ref|NP_494786.2| TWiK family of potassium channels family member (twk-2) [Caenorhabditis elegans] 0.0
blast H88124 protein T12C9.3 [imported] - Caenorhabditis elegans 0.0
blast CAE67892 Hypothetical protein CBG13488 [Caenorhabditis briggsae] 0.0
blast CAE60144 Hypothetical protein CBG03693 [Caenorhabditis briggsae] 0.0
blast CAE60144 Hypothetical protein CBG03693 [Caenorhabditis briggsae] 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR005560 EMBL-EBI Protein of unknown function DUF326 7.8E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494786.2 3 13-32,42-59,66-88
T12C9.3 3 13-32,42-59,66-88
SNAP00000005698 6 33-55,164-186,196-218,278-300,304-326,333-355
Sequence
Target Sequence 
>C. ELEGANS:179971_AT
gtcgccccaacggaagtagcggacacatcatgtttcgtttgcgattcgaatctggtcaca
atgcaaatgatagaagaagatgcagatcatattcactggaaacagacaatgtcaactaca
cattactatgcaacagacaattggaaagatgatttctatcgggtcactcctctcacgtgt
agaggagttcttcgtggtgcatatagagttgggaagagccggtgtggcaagattcagaga
gttgatattattagggtgtatcgtgtgactcgtcatttcagttgctggaagacagttcca
gtttttcatcgagtcatctctctagttgaagctgcgttaccttacaaacaaatgaccaca
aaacaaaagcaacttttgaaagcaaacggaaataaattgatcaatcggttgtttattcaa
tattattggagatttaatcaaagtttcctgaaccgcgaacaagtgctcagcgaatacgcg
gaatcgacgaaaagaactcgtgccaaaaagtcgtggatccgagttgtgaaaaacgtggca
cgtctgcgagctgcgacgagtatccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGCCCCAACGGAAGTAGCGGACA 192 473 5149 Antisense
GGACACATCATGTTTCGTTTGCGAT 432 525 5169 Antisense
GCGATTCGAATCTGGTCACAATGCA 445 291 5189 Antisense
AGATGATTTCTATCGGGTCACTCCT 18 69 5295 Antisense
AGAGGAGTTCTTCGTGGTGCATATA 430 67 5329 Antisense
GTGACTCGTCATTTCAGTTGCTGGA 192 483 5413 Antisense
GTTCCAGTTTTTCATCGAGTCATCT 275 441 5443 Antisense
GAGTCATCTCTCTAGTTGAAGCTGC 103 401 5459 Antisense
GTTGAAGCTGCGTTACCTTACAAAC 571 437 5473 Antisense
GTTTCCTGAACCGCGAACAAGTGCT 429 447 5591 Antisense
TCTGCGAGCTGCGACGAGTATCCTA 543 609 5691 Antisense
Sequence Source Affymetrix Proprietary Database