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[179970_at]

Description C27F2.6 /REP_DB=WormBase Gene ID /WP=CE01173 /TR=Q18261 /GB=AAA81426.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 11      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 3.2
(-3)
A 		14.3
(-1.5)
A 		5.2
(-2.7)
A 		47.5
(3)
A 		44
B 21.2
(0.8)
A 		11.8
(-3)
A 		12
(-2.9)
A 		26.8
(3)
A 		15
C 14.9
(-1.7)
A 		11.3
(-2)
A 		1.2
(-3)
A 		64.7
(3)
A 		64
avr 13.1
(-2) 		12.5
(-2) 		6.1
(-3) 		46.3
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 5.7
(-1.4)
A 		1.6
(-3)
A 		14.3
(1.9)
A 		5.8
(-1.4)
A 		17.2
(3)
A 		6
(-1.3)
A 		16
B 2.9
(-2.9)
A 		28.3
(3)
A 		16.8
(0.3)
A 		2.5
(-3)
A 		6.3
(-2.1)
A 		3.2
(-2.8)
A 		26
C 1.8
(-2.6)
A 		3.7
(-1.6)
A 		3.1
(-1.9)
A 		12.3
(3)
A 		1
(-3)
A 		9.8
(1.7)
A 		11
avr 3.5
(-3) 		11.2
(2.8) 		11.4
(3) 		6.9
(-0.4) 		8.2
(0.6) 		6.3
(-0.9) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.2
(-3)
A 		3.1
(-1)
A 		1.4
(-2.8)
A 		6.9
(3)
A 		6
B 6.8
(3)
A 		2.9
(-2.9)
A 		2.8
(-3)
A 		5.7
(1.4)
A 		4
C 4.9
(-0.4)
A 		3.2
(-1.4)
A 		0.4
(-3)
A 		10.9
(3)
A 		11
avr 4.3
(-0.3) 		3.1
(-1.5) 		1.5
(-3) 		7.8
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1.1
(-2.3)
A 		0.6
(-3)
A 		3.1
(0.3)
A 		1.5
(-1.8)
A 		5.2
(3)
A 		1.5
(-1.8)
A 		5
B 1
(-2.8)
A 		9.4
(3)
A 		4.1
(-0.7)
A 		0.7
(-3)
A 		2.6
(-1.7)
A 		1.2
(-2.7)
A 		9
C 0.7
(-2.2)
A 		1.1
(-1.4)
A 		0.8
(-2)
A 		2.5
(1.4)
A 		0.3
(-3)
A 		3.3
(3)
A 		3
avr 0.9
(-3) 		3.7
(3) 		2.7
(0.9) 		1.6
(-1.5) 		2.7
(0.9) 		2
(-0.6) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179970_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7026
Sequence Type Exemplar sequence
Representative Public ID C27F2.6 NCBI
Target Description C27F2.6 /REP_DB=WormBase Gene ID /WP=CE01173 /TR=Q18261 /GB=AAA81426.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065654(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065654 NCBI Caenorhabditis elegans C27F2.6 (C27F2.6) mRNA, complete cds. 11/11 A 
C27F2.6 ENSEMBL cdna:known chromosome:CEL140:III:4979757:4980379:-1 gene:C27F2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4979755-4980378(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27F2.6
Entrez Gene 182966 Entrez gene
SwissProt Q18261 EMBL-EBI
WormBase CE01173 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498055 C27F2.6 [Caenorhabditis elegans] gb|AAA81426.1| Hypothetical protein C27F2.6 [Caenorhabditis elegans] 4.0E-75
blast CAA84797 Hypothetical protein F17C8.5 [Caenorhabditis elegans] ref|NP_497973.1| TWiK family of potassium channels family member (twk-6) [Caenorhabditis elegans] 1.0E-58
blast CAE71315 Hypothetical protein CBG18207 [Caenorhabditis briggsae] 4.0E-30
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498055.1 3 20-39,46-68,72-94
C27F2.6 3 20-39,46-68,72-94
Sequence
Target Sequence 
>C. ELEGANS:179970_AT
cggacacgagtttctggctactcgcgagtaattcactccaacgtgataatttgatattcc
tatcttcacttcttctctgctcaatatctcttctgagttccagtgcacttttctcttcaa
ttgagaatatttcatatcaaagttccgcttatttcggatttatgactatttttggaatta
gtttcggagatattgttcctacaaatttagtatggttcagtggatattgtttgttcttct
taatttctgacgtgctgtcaaatcatattttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGACACGAGTTTCTGGCTACTCGC 418 243 20 Antisense
GAGTTTCTGGCTACTCGCGAGTAAT 281 399 27 Antisense
ACTCGCGAGTAATTCACTCCAACGT 35 99 39 Antisense
AATTCACTCCAACGTGATAATTTGA 315 173 49 Antisense
GATAATTTGATATTCCTATCTTCAC 167 423 64 Antisense
AATATTTCATATCAAAGTTCCGCTT 2 175 145 Antisense
AAGTTCCGCTTATTTCGGATTTATG 531 155 159 Antisense
ATTTCGGATTTATGACTATTTTTGG 201 17 170 Antisense
AGTGGATATTGTTTGTTCTTCTTAA 266 59 238 Antisense
GTTCTTCTTAATTTCTGACGTGCTG 257 443 252 Antisense
TGACGTGCTGTCAAATCATATTTTT 278 571 267 Antisense
Sequence Source Affymetrix Proprietary Database