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[179961_at]

Description B0454.1 /REP_DB=WormBase Gene ID /WP=CE20455 /TR=O17174 /GB=AAB70947.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 29      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 71      6grp_total > 29      4grp_total > 43      6grp_cgc > 02      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 431.2
(-3)
P 		581.8
(2.8)
P 		495.1
(-0.5)
P 		587
(3)
P 		156
B 483.2
(2.7)
P 		486.5
(3)
P 		452.4
(-0.5)
P 		427.8
(-3)
P 		59
C 498.1
(1.4)
P 		530.9
(3)
P 		426.7
(-1.9)
P 		404.1
(-3)
P 		127
avr 470.8
(-2) 		533.1
(3) 		458.1
(-3) 		473
(-1.8) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 434.5
(3)
P 		288.8
(-1.9)
P 		360.2
(0.5)
P 		254.5
(-3)
P 		386.5
(1.4)
P 		343.6
(-0)
P 		180
B 471
(3)
P 		322.4
(-3)
P 		335.1
(-2.5)
P 		343.6
(-2.1)
P 		429.5
(1.3)
P 		351.2
(-1.8)
P 		149
C 332.5
(1.5)
P 		375.7
(3)
P 		269.1
(-0.7)
P 		202.2
(-3)
P 		369.3
(2.8)
P 		304.3
(0.5)
P 		174
avr 412.7
(3) 		329
(-0.4) 		321.5
(-0.8) 		266.8
(-3) 		395.1
(2.3) 		333
(-0.3) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 157
(3)
P 		126.3
(0.4)
P 		129.4
(0.7)
P 		85.9
(-3)
P 		71
B 154.1
(3)
P 		120.2
(-0.2)
P 		104.8
(-1.7)
P 		91.2
(-3)
P 		63
C 164.9
(3)
P 		152.6
(2.2)
P 		148.7
(2)
P 		68.1
(-3)
P 		97
avr 158.7
(3) 		133
(1) 		127.6
(0.6) 		81.7
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 80.1
(-1.2)
P 		102.7
(1.4)
P 		78.4
(-1.4)
P 		64.6
(-3)
P 		116.6
(3)
P 		83.7
(-0.8)
P 		52
B 163.9
(2.3)
P 		107.3
(-1.3)
P 		81.4
(-3)
P 		91
(-2.4)
P 		174.5
(3)
P 		131
(0.2)
P 		93
C 123.3
(3)
P 		114.6
(2.4)
P 		68.5
(-1)
P 		40.7
(-3)
P 		119.5
(2.7)
P 		101.9
(1.4)
P 		83
avr 122.4
(1.8) 		108.2
(0.6) 		76.1
(-2.1) 		65.4
(-3) 		136.9
(3) 		105.5
(0.4) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179961_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4636
Sequence Type Exemplar sequence
Representative Public ID B0454.1 NCBI
Target Description B0454.1 /REP_DB=WormBase Gene ID /WP=CE20455 /TR=O17174 /GB=AAB70947.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062035(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062035 NCBI Caenorhabditis elegans B0454.1 (B0454.1) mRNA, complete cds. 11/11 None
SNAP00000041876 ENSEMBL cdna:SNAP chromosome:CEL140:II:3058186:3062030:1 11/11 None
GENEFINDER00000041891 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3058233:3062030:1 11/11 None
B0454.1 ENSEMBL cdna:known chromosome:CEL140:II:3056820:3060173:1 gene:B0454.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062036 6/11 Cross Hyb Matching Probes None
Y25C1A.6.1 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3056828-3060120(+) 97.33 100.0
Public Domain and Genome References
Gene Title
Ensembl B0454.1
Entrez Gene 173652 Entrez gene
SwissProt O17174 EMBL-EBI
WormBase CE20455 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 0.0
blast D88088 protein B0454.1 [imported] - Caenorhabditis elegans 1.0E-147
blast NP_494437 Y25C1A.6 [Caenorhabditis elegans] gb|AAD12837.1| Hypothetical protein Y25C1A.6 [Caenorhabditis elegans] 1.0E-107
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 1.0E-102
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 9.0E-94
blast D88088 protein B0454.1 [imported] - Caenorhabditis elegans 9.0E-63
blast CAE72459 Hypothetical protein CBG19631 [Caenorhabditis briggsae] 2.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 1.5E-12
Sequence
Target Sequence 
>C. ELEGANS:179961_AT
tggattctggggagctcatggagcccatggagcccatggattctacaatggatgagatgt
gcgtcgaggaggagccctacgaggagacagggtccaattggagcgatccggcgccggaac
catcccaatccaaatcccagtccccagaagccaagtaccctcaagcctacctactacctg
aggcggacgaagtctacaatcctgacgatttctatcaagaggaacatgaatccgcatcaa
acgccatgtatcggatcgctttctcacagcagtacggtggcggcgggtccccagccgtgc
agaagcccgtcacttttagtgctcagccggcgccggcgccagttagagaggccccaagcc
cagttgtggagaatgttagttcatcgagtttcaccccgaagcccccggccatgatcaaca
attttggtgaggagatgaaccaaataacataccaagcgatccgtattgcccgagagcagc
cggaacgtctgaaattgctccgtaaggcacttttcgacgttgtcctggcgtttgatcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGATTCTGGGGAGCTCATGGAGCC 575 549 578 Antisense
CGTCGAGGAGGAGCCCTACGAGGAG 292 245 639 Antisense
CAAGCCTACCTACTACCTGAGGCGG 215 185 739 Antisense
GGCGGACGAAGTCTACAATCCTGAC 45 537 759 Antisense
GGAACATGAATCCGCATCAAACGCC 260 529 798 Antisense
AAACGCCATGTATCGGATCGCTTTC 42 127 816 Antisense
GCCCCAAGCCCAGTTGTGGAGAATG 655 283 928 Antisense
ATGTTAGTTCATCGAGTTTCACCCC 247 45 950 Antisense
ACATACCAAGCGATCCGTATTGCCC 73 105 1024 Antisense
GTAAGGCACTTTTCGACGTTGTCCT 115 463 1079 Antisense
GACGTTGTCCTGGCGTTTGATCAGA 40 379 1093 Antisense
Sequence Source Affymetrix Proprietary Database