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[179950_at]

Description F53C3.3 /REP_DB=WormBase Gene ID /WP=CE19441 /TR=Q9TXT6 /GB=AAC67454.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 44      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 51.2
(3)
A 		41.2
(1.6)
A 		14.2
(-2.3)
A 		9.3
(-3)
A 		42
B 15
(2.3)
A 		15.7
(3)
A 		9.3
(-3)
A 		12.5
(0)
A 		6
C 46.7
(0.4)
A 		70.1
(3)
A 		47.8
(0.5)
A 		15.5
(-3)
A 		55
avr 37.6
(2.1) 		42.3
(3) 		23.8
(-0.7) 		12.4
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 22.2
(0.1)
A 		37.9
(3)
A 		21.2
(-0.1)
A 		5.8
(-3)
A 		6.9
(-2.8)
A 		11.4
(-2)
A 		32
B 48.7
(3)
A 		14.7
(-1.6)
A 		9.5
(-2.3)
A 		4.1
(-3)
A 		4.8
(-2.9)
A 		30.9
(0.6)
A 		45
C 64.2
(0.3)
A 		22.5
(-2.3)
A 		45.3
(-0.9)
A 		109.1
(3)
A 		22
(-2.3)
A 		10.5
(-3)
A 		99
avr 45
(3) 		25
(-0.6) 		25.3
(-0.5) 		39.7
(2.1) 		11.2
(-3) 		17.6
(-1.9) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 18.6
(3)
A 		8.9
(-0.4)
A 		3.7
(-2.2)
A 		1.4
(-3)
A 		17
B 4.8
(3)
A 		3.9
(0.9)
A 		2.2
(-3)
A 		2.7
(-1.8)
A 		3
C 15.5
(1.4)
A 		20.1
(3)
A 		16.7
(1.8)
A 		2.6
(-3)
A 		18
avr 13
(3) 		11
(1.9) 		7.5
(-0.1) 		2.2
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 4.1
(-1.7)
A 		13.5
(3)
A 		4.6
(-1.5)
A 		1.5
(-3)
A 		2.1
(-2.7)
A 		2.8
(-2.4)
A 		12
B 17
(3)
A 		4.9
(-1.6)
A 		2.3
(-2.5)
A 		1.1
(-3)
A 		2
(-2.7)
A 		11.5
(0.9)
A 		16
C 23.8
(3)
A 		6.9
(-2)
A 		11.5
(-0.6)
A 		21.9
(2.4)
A 		7.1
(-1.9)
A 		3.5
(-3)
A 		20
avr 15
(3) 		8.4
(-0.5) 		6.1
(-1.7) 		8.2
(-0.6) 		3.7
(-3) 		5.9
(-1.8) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179950_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4166
Sequence Type Exemplar sequence
Representative Public ID F53C3.3 NCBI
Target Description F53C3.3 /REP_DB=WormBase Gene ID /WP=CE19441 /TR=Q9TXT6 /GB=AAC67454.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062290(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062290 NCBI Caenorhabditis elegans F53C3.3 (F53C3.3) mRNA, complete cds. 11/11 None
SNAP00000027224 ENSEMBL cdna:SNAP chromosome:CEL140:II:3902875:3904536:1 11/11 None
GENEFINDER00000027231 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3902875:3904536:1 11/11 None
F53C3.3 ENSEMBL cdna:known chromosome:CEL140:II:3902860:3904536:1 gene:F53C3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3902873-3903634(+) 87.27 100.0
Public Domain and Genome References
Gene Title
Ensembl F53C3.3
Entrez Gene 173732 Entrez gene
SwissProt Q9TXT6 EMBL-EBI
WormBase CE35939 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67454 Hypothetical protein F53C3.3 [Caenorhabditis elegans] ref|NP_494691.2| F53C3.3 [Caenorhabditis elegans] 1.0E-102
blast AAK73869 Hypothetical protein F53C3.6a [Caenorhabditis elegans] ref|NP_494687.2| F53C3.6a [Caenorhabditis elegans] 1.0E-62
blast AAC67455 Hypothetical protein F53C3.4 [Caenorhabditis elegans] ref|NP_494690.2| F53C3.4 [Caenorhabditis elegans] 2.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 1.5E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494691.2 1 172-194
F53C3.3 1 172-194
SNAP00000027224 1 172-194
GENEFINDER00000027231 1 172-194
Sequence
Target Sequence 
>C. ELEGANS:179950_AT
ggtggtgcgcgaggtggtgtaggcggacgcggtggaggtggcagcttcagatcaggagga
gcccgatcgagctccagtagttttaggggaggaagtagtcactatagctcgagtttccgg
cagaatgtattccacaccagctcaactgctaagacattcgcctattctccaatgactcgc
actcatctcatcatctcacctgcttctccgatgatttacggaaactaccattactattgg
gcaggacatcatgtgaacacgacagaaaaaccgtataagtgtgattatcaaatatctgat
gaggatatggaattgagaaatgtaacatttgcaaatggtacaagaccagcaaatattact
tttgggtgcgcagaatatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGGTGCGCGAGGTGGTGTAGGCG 609 499 106 Antisense
TGTAGGCGGACGCGGTGGAGGTGGC 376 549 123 Antisense
GGAGGTGGCAGCTTCAGATCAGGAG 360 517 139 Antisense
CAGGAGGAGCCCGATCGAGCTCCAG 210 209 158 Antisense
GATCGAGCTCCAGTAGTTTTAGGGG 674 417 170 Antisense
AGTCACTATAGCTCGAGTTTCCGGC 470 59 202 Antisense
TTCCACACCAGCTCAACTGCTAAGA 340 701 235 Antisense
TGCTAAGACATTCGCCTATTCTCCA 237 579 252 Antisense
ACCTGCTTCTCCGATGATTTACGGA 63 89 303 Antisense
TTTACGGAAACTACCATTACTATTG 508 667 320 Antisense
ATTACTTTTGGGTGCGCAGAATATC 244 13 460 Antisense
Sequence Source Affymetrix Proprietary Database