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[179943_at]

Description T06D4.4 /REP_DB=WormBase Gene ID /WP=CE13307 /TR=O16652 /GB=AAB66123.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 44      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 48.1
(3)
A 		36.2
(1.3)
A 		21.8
(-0.7)
A 		4.9
(-3)
A 		43
B 26
(-1.3)
A 		68.8
(3)
A 		46.8
(0.8)
A 		9.2
(-3)
A 		60
C 56.5
(-1.2)
A 		37.4
(-3)
A 		52.8
(-1.6)
A 		101.8
(3)
A 		64
avr 43.5
(0.3) 		47.5
(3) 		40.5
(-1.7) 		38.6
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 41.2
(0)
A 		12
(-2.9)
A 		18.9
(-2.2)
A 		10.6
(-3)
A 		71.4
(3)
A 		40.1
(-0.1)
A 		61
B 24.8
(-1.5)
A 		6.7
(-3)
A 		79.4
(3)
A 		17.5
(-2.1)
A 		48.9
(0.5)
A 		72.5
(2.4)
P 		73
C 36.8
(-2.3)
A 		34.2
(-2.5)
A 		69.8
(0.9)
A 		29
(-3)
A 		53.2
(-0.7)
A 		91.4
(3)
A 		62
avr 34.3
(-1) 		17.6
(-3) 		56
(1.6) 		19
(-2.8) 		57.8
(1.8) 		68
(3) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 17.5
(3)
A 		7.9
(-0.4)
A 		5.7
(-1.2)
A 		0.7
(-3)
A 		17
B 8.3
(-0.5)
A 		17
(3)
A 		10.9
(0.6)
A 		2
(-3)
A 		15
C 18.7
(3)
A 		10.8
(-3)
A 		18.4
(2.8)
A 		17.2
(1.9)
A 		8
avr 14.8
(3) 		11.9
(0.9) 		11.7
(0.7) 		6.6
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 7.6
(-1.4)
A 		4.3
(-2.5)
A 		4.1
(-2.6)
A 		2.7
(-3)
A 		21.6
(3)
A 		9.8
(-0.7)
A 		19
B 8.6
(-1.5)
A 		2.2
(-3)
A 		19.3
(1.1)
A 		4.6
(-2.4)
A 		19.9
(1.3)
A 		27
(3)
P 		25
C 13.7
(-1.1)
A 		10.4
(-1.9)
A 		17.8
(-0.1)
A 		5.8
(-3)
A 		17.2
(-0.2)
A 		30.6
(3)
A 		25
avr 10
(-1.1) 		5.6
(-2.6) 		13.7
(0.1) 		4.4
(-3) 		19.6
(2) 		22.5
(3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179943_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4974
Sequence Type Exemplar sequence
Representative Public ID T06D4.4 NCBI
Target Description T06D4.4 /REP_DB=WormBase Gene ID /WP=CE13307 /TR=O16652 /GB=AAB66123.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062129(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062129 NCBI Caenorhabditis elegans T06D4.4 (T06D4.4) mRNA, complete cds. 11/11 None
GENEFINDER00000039462 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3387949:3395528:-1 11/11 None
T06D4.4 ENSEMBL cdna:known chromosome:CEL140:II:3391959:3395026:-1 gene:T06D4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3391957-3395025(-) 97.32 100.0
Public Domain and Genome References
Gene Title
Ensembl T06D4.4
Entrez Gene 188176 Entrez gene
SwissProt O16652 EMBL-EBI
WormBase CE13307 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66123 Hypothetical protein T06D4.4 [Caenorhabditis elegans] ref|NP_494530.1| T06D4.4 [Caenorhabditis elegans] 0.0
blast AAB66077 Hypothetical protein F18A12.5 [Caenorhabditis elegans] ref|NP_494531.2| F18A12.5 [Caenorhabditis elegans] 0.0
blast AAB66125 Hypothetical protein T06D4.3 [Caenorhabditis elegans] ref|NP_494529.2| T06D4.3 [Caenorhabditis elegans] 0.0
blast AAB66078 Hypothetical protein F18A12.6 [Caenorhabditis elegans] ref|NP_494532.1| F18A12.6 [Caenorhabditis elegans] 1.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 5.3E-97
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.7E-7
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.3E-12
Pfam IPR008753 EMBL-EBI Peptidase M13 5.3E-97
Pfam IPR008753 EMBL-EBI Peptidase M13 8.9E-10
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.7E-7
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.4E-12
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.8E-64
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494530.1 1 33-52
T06D4.4 1 33-52
GENEFINDER00000039462 1 185-204
Sequence
Target Sequence 
>C. ELEGANS:179943_AT
cattgctccgatcattgatggcccttactttgatacctcttaccctaactacgtgaacat
tttcttcatgggattcatcattagccgagagctgggccgctccttggattcagtgaggat
tcggaaggatgagaagggccacactgagaattggctgaagtcggagagcttgaagagatt
tgacaataacgtgaaatgttggcgtaaaaagatcactggaactcgttttctgaacgagat
gctcgctgaccatgttggacatgaagcgtcttttatggctttgaagaaatgggaaaagga
aatgaatgggacgatagtagagaagattcgtggatatgagagtatgacgatggagcagat
tttctttcaccttcaagctttgtacttctgccgtcccccgggtggttcaaccggtgaact
aaccgccaaagatgctcgccaacattactcgactcgtgtcaatgaagcattcaagaacat
gccacaattcgccaaagctttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATTGCTCCGATCATTGATGGCCCT 72 217 1506 Antisense
TTGATGGCCCTTACTTTGATACCTC 55 705 1520 Antisense
GGGATTCATCATTAGCCGAGAGCTG 259 497 1575 Antisense
GCCGCTCCTTGGATTCAGTGAGGAT 685 283 1601 Antisense
GATCACTGGAACTCGTTTTCTGAAC 459 421 1716 Antisense
GATGCTCGCTGACCATGTTGGACAT 611 413 1743 Antisense
TGGAGCAGATTTTCTTTCACCTTCA 403 549 1856 Antisense
CACCTTCAAGCTTTGTACTTCTGCC 690 201 1873 Antisense
GAACTAACCGCCAAAGATGCTCGCC 242 349 1921 Antisense
AAGATGCTCGCCAACATTACTCGAC 226 155 1934 Antisense
CATGCCACAATTCGCCAAAGCTTTT 324 211 1983 Antisense
Sequence Source Affymetrix Proprietary Database