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[179939_at]

Description F22E5.6 /REP_DB=WormBase Gene ID /WP=CE09558 /TR=O16711 /GB=AAB66172.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 15      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 32.4
(3)
A 		10.4
(-2.2)
A 		7.2
(-3)
A 		26.2
(1.5)
A 		25
B 28.2
(-2.5)
A 		26.7
(-3)
A 		43.8
(3)
M 		27.4
(-2.8)
A 		17
C 34.6
(3)
A 		7
(-2.8)
A 		5.9
(-3)
A 		32.3
(2.5)
A 		29
avr 31.7
(3) 		14.7
(-3) 		19
(-1.5) 		28.6
(1.9) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 49.2
(3)
A 		3.6
(-3)
A 		22.2
(-0.6)
A 		9.9
(-2.2)
A 		9
(-2.3)
A 		7.5
(-2.5)
A 		46
B 7.1
(-2.1)
A 		5.3
(-2.5)
A 		35.3
(3)
A 		9.1
(-1.8)
A 		3.9
(-2.7)
A 		2.3
(-3)
A 		33
C 26.6
(3)
A 		19.8
(1.1)
A 		7
(-2.6)
A 		25.7
(2.7)
A 		5.5
(-3)
A 		8.4
(-2.2)
A 		21
avr 27.6
(3) 		9.6
(-2) 		21.5
(1.3) 		14.9
(-0.5) 		6.1
(-3) 		6.1
(-3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 11.8
(3)
A 		2.3
(-2.8)
A 		1.9
(-3)
A 		3.8
(-1.8)
A 		10
B 9
(1.5)
A 		6.6
(-1.9)
A 		10.1
(3)
M 		5.8
(-3)
A 		4
C 11.5
(3)
A 		2
(-3)
A 		2
(-3)
A 		5.4
(-0.9)
A 		10
avr 10.8
(3) 		3.6
(-3) 		4.7
(-2.1) 		5
(-1.8) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 9.1
(3)
A 		1.3
(-3)
A 		4.8
(-0.3)
A 		2.5
(-2.1)
A 		2.7
(-1.9)
A 		1.8
(-2.6)
A 		8
B 2.5
(-1.7)
A 		1.8
(-2.2)
A 		8.6
(3)
A 		2.4
(-1.8)
A 		1.6
(-2.4)
A 		0.8
(-3)
A 		8
C 9.9
(3)
A 		6.1
(0.2)
A 		1.8
(-3)
A 		5.2
(-0.5)
A 		1.8
(-3)
A 		2.8
(-2.3)
A 		8
avr 7.2
(3) 		3.1
(-1.6) 		5.1
(0.7) 		3.4
(-1.2) 		2
(-2.8) 		1.8
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179939_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5254
Sequence Type Exemplar sequence
Representative Public ID F22E5.6 NCBI
Target Description F22E5.6 /REP_DB=WormBase Gene ID /WP=CE09558 /TR=O16711 /GB=AAB66172.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061919(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061919 NCBI Caenorhabditis elegans F22E5.6 (F22E5.6) mRNA, complete cds. 11/11 None
SNAP00000023609 ENSEMBL cdna:SNAP chromosome:CEL140:II:2649257:2650716:1 11/11 None
GENEFINDER00000023632 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2649871:2650716:1 11/11 None
F22E5.6 ENSEMBL cdna:known chromosome:CEL140:II:2649871:2650716:1 gene:F22E5.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062072 1/11 Cross Hyb Matching Probes A 
SNAP00000024292 1/11 Cross Hyb Matching Probes None
GENEFINDER00000024314 1/11 Cross Hyb Matching Probes None
ZC239.12 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2649869-2650715(+) 100.0 100.0
chrII:3207398-3208745(-) 90.95 90.95
Public Domain and Genome References
Gene Title
Ensembl F22E5.6
Entrez Gene 184835 Entrez gene
SwissProt O16711 EMBL-EBI
WormBase CE35503 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494320 F22E5.6 [Caenorhabditis elegans] gb|AAB66172.2| Hypothetical protein F22E5.6 [Caenorhabditis elegans] 1.0E-129
blast NP_494320 F22E5.6 [Caenorhabditis elegans] gb|AAB66172.2| Hypothetical protein F22E5.6 [Caenorhabditis elegans] 1.0E-127
blast AAB37951 Hypothetical protein ZC239.12 [Caenorhabditis elegans] ref|NP_494473.1| ZC239.12 [Caenorhabditis elegans] 1.0E-114
blast AAB37951 Hypothetical protein ZC239.12 [Caenorhabditis elegans] ref|NP_494473.1| ZC239.12 [Caenorhabditis elegans] 1.0E-112
blast NP_494320 F22E5.6 [Caenorhabditis elegans] gb|AAB66172.2| Hypothetical protein F22E5.6 [Caenorhabditis elegans] 1.0E-105
blast AAB37951 Hypothetical protein ZC239.12 [Caenorhabditis elegans] ref|NP_494473.1| ZC239.12 [Caenorhabditis elegans] 1.0E-91
blast AAC68757 Hypothetical protein C40A11.6 [Caenorhabditis elegans] ref|NP_494197.1| C40A11.6 [Caenorhabditis elegans] 3.0E-50
blast AAC68757 Hypothetical protein C40A11.6 [Caenorhabditis elegans] ref|NP_494197.1| C40A11.6 [Caenorhabditis elegans] 5.0E-49
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.2E-45
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 1.3E-24
Sequence
Target Sequence 
>C. ELEGANS:179939_AT
agaccgatctccgagacattttgaaaaaattttgaattatttgagagatggtgcagacgt
ggatttacttccagaatctgaaaaagaagttcgagaaattttgaaagaagctcaattcta
tctattggagggtttaatggagctctgcaagcggtcaagttgtaaaattcgaacttttga
atcttatcaccatttgctgaaattgatagcggaagctggaaagccggttctagtgatgtt
ctacctggtcaaagacaatcgaatagttcacgttcccaacaatttcgaatttctggattt
tttggaaaaacaccaaggaaaattagacatttatttcagtgagtttggacaaaaaaaaga
atccccatacgaaacaacacaccaaaatccatcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACCGATCTCCGAGACATTTTGAA 436 69 165 Antisense
GAAGCTCAATTCTATCTATTGGAGG 277 343 271 Antisense
TGGAGGGTTTAATGGAGCTCTGCAA 387 539 290 Antisense
GGAGCTCTGCAAGCGGTCAAGTTGT 357 521 303 Antisense
GAATCTTATCACCATTTGCTGAAAT 245 329 343 Antisense
GCGGAAGCTGGAAAGCCGGTTCTAG 514 287 373 Antisense
GCCGGTTCTAGTGATGTTCTACCTG 31 285 387 Antisense
GTGATGTTCTACCTGGTCAAAGACA 177 483 397 Antisense
GACAATCGAATAGTTCACGTTCCCA 694 363 418 Antisense
GTTCACGTTCCCAACAATTTCGAAT 448 445 430 Antisense
GAAACAACACACCAAAATCCATCAC 27 357 535 Antisense
Sequence Source Affymetrix Proprietary Database