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[179935_at]

Description C27F2.8 /REP_DB=WormBase Gene ID /WP=CE26876 /TR=Q18264 /GB=AAA81429.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 67      4grp_highest_8_8 > 27      4grp_high_8_8 > 57      6grp_highest_nonorm > 73      6grp_total > 69      4grp_total > 63      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 382
1 2 3 4 range
A 524.8
(3)
P 		398.2
(-0.6)
P 		523.2
(3)
P 		313.5
(-3)
P 		211
B 514.9
(0.7)
P 		406
(-3)
P 		583.1
(3)
P 		513.8
(0.7)
P 		177
C 586.2
(0.8)
P 		534.1
(-0.3)
P 		695.8
(3)
P 		402.2
(-3)
P 		294
avr 542
(1.2) 		446.1
(-1.9) 		600.7
(3) 		409.8
(-3) 		191

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 296
1 2 3 4 5 6 range
A 435.3
(-1.3)
P 		645.6
(3)
P 		349.3
(-3)
P 		616.9
(2.4)
P 		451.3
(-0.9)
P 		607.7
(2.2)
P 		296
B 519.1
(2.1)
P 		412
(-3)
P 		537.1
(3)
P 		454
(-1)
P 		459.5
(-0.7)
P 		517.5
(2.1)
P 		125
C 597.3
(2)
P 		477
(-2.9)
P 		570.9
(0.9)
P 		474.5
(-3)
P 		621.4
(3)
P 		543.5
(-0.2)
P 		147
avr 517.2
(-0.3) 		511.5
(-0.8) 		485.8
(-3) 		515.1
(-0.5) 		510.7
(-0.9) 		556.2
(3) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 191.1
(3)
P 		86.4
(-1.3)
P 		136.8
(0.8)
P 		45.9
(-3)
P 		145
B 164.2
(3)
P 		100.3
(-3)
P 		135.1
(0.3)
P 		109.5
(-2.1)
P 		64
C 194.1
(1.3)
P 		153.6
(-0.1)
P 		242.5
(3)
P 		67.8
(-3)
P 		175
avr 183.1
(3) 		113.4
(-0.8) 		171.5
(2.4) 		74.4
(-3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 154
1 2 3 4 5 6 range
A 80.2
(-2.8)
P 		229.6
(3)
P 		76.1
(-3)
P 		156.5
(0.1)
P 		136.2
(-0.7)
P 		147.9
(-0.2)
P 		154
B 180.6
(2)
P 		137.1
(-1.6)
P 		130.4
(-2.2)
P 		120.2
(-3)
P 		186.7
(2.5)
P 		193
(3)
P 		73
C 221.4
(3)
P 		145.6
(-0.6)
P 		145.3
(-0.6)
P 		95.4
(-3)
P 		201.1
(2)
P 		181.9
(1.1)
P 		126
avr 160.7
(1.5) 		170.8
(2.6) 		117.3
(-3) 		124
(-2.3) 		174.7
(3) 		174.3
(3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179935_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7333
Sequence Type Exemplar sequence
Representative Public ID C27F2.8 NCBI
Target Description C27F2.8 /REP_DB=WormBase Gene ID /WP=CE26876 /TR=Q18264 /GB=AAA81429.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065658(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065658 NCBI Caenorhabditis elegans C27F2.8 (C27F2.8) mRNA, complete cds. 11/11 A 
C27F2.8 ENSEMBL cdna:known chromosome:CEL140:III:4991409:4997703:-1 gene:C27F2.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4991522-4997702(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27F2.8
Entrez Gene 175677 Entrez gene
WormBase CE39000 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597141_AT similar to RW1 protein cfa
CANINE_2:CFAAFFX.4415.1.S1_AT similar to RW1 protein cfa
DROSOPHILA_2:1629053_AT dm
DROSGENOME1:141227_AT dm
CHICKEN:GGA.12674.2.A1_A_AT RW1 protein gga
CHICKEN:GGA.12674.2.A1_AT RW1 protein gga
CHICKEN:GGA.12674.3.S1_A_AT RW1 protein gga
CHICKEN:GGA.17653.1.S1_S_AT RW1 protein gga
CHICKEN:GGAAFFX.25052.1.S1_S_AT RW1 protein gga
CHICKEN:GGA.12674.3.A1_A_AT RW1 protein gga
HG-U133A:212507_AT RW1 protein hs
HG-U133A_2:212507_AT RW1 protein hs
HG-U133_PLUS_2:212507_AT RW1 protein hs
HUGENEFL:D87446_AT RW1 protein hs
U133_X3P:HS.75912.0.A1_3P_AT RW1 protein hs
U133_X3P:HS.160792.0.A1_3P_AT RW1 protein hs
HG-U133_PLUS_2:235972_AT RW1 protein hs
HG-U133B:235972_AT RW1 protein hs
HG-U95AV2:36971_AT RW1 protein hs
HG-U95D:91638_AT RW1 protein hs
MG-U74AV2:102336_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed mm
MOE430A:1448396_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed mm
MOUSE430A_2:1448396_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed mm
MOUSE430_2:1448396_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed mm
MOE430B:1457822_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed (D1Bwg0491e), mRNA mm
MOUSE430_2:1457822_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed (D1Bwg0491e), mRNA mm
MU19KSUBA:TC17033_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed (D1Bwg0491e), mRNA mm
MU19KSUBB:TC26047_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed (D1Bwg0491e), mRNA mm
MU19KSUBC:TC39208_AT DNA segment, Chr 1, Brigham & Women's Genetics 0491 expressed (D1Bwg0491e), mRNA mm
RAE230B:1394864_AT similar to RW1 protein (predicted) rn
RAT230_2:1394864_AT similar to RW1 protein (predicted) rn
RAE230A:1372554_AT Similar to RW1 protein (predicted) rn
RAT230_2:1372554_AT Similar to RW1 protein (predicted) rn
RAE230B:1390963_AT Similar to RW1 protein (predicted) rn
RAT230_2:1390963_AT Similar to RW1 protein (predicted) rn
RAE230B:1396447_AT Similar to RW1 protein (predicted) rn
RAT230_2:1396447_AT Similar to RW1 protein (predicted) rn
RG-U34B:RC_AA943110_AT Similar to RW1 protein (predicted) rn
RG-U34B:RC_AA997034_AT Similar to RW1 protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast NP_498059 C27F2.8 [Caenorhabditis elegans] gb|AAA81429.2| Hypothetical protein C27F2.8 [Caenorhabditis elegans] 0.0
blast CAE70089 Hypothetical protein CBG16531 [Caenorhabditis briggsae] 0.0
blast XP_371542 PREDICTED: RW1 protein [Homo sapiens] 1.0E-107
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498059.1 2 1054-1076,1143-1165
C27F2.8 2 1054-1076,1143-1165
Sequence
Target Sequence 
>C. ELEGANS:179935_AT
gggccgttgagtgcggaacagatggaagatcttattcgacaagcttctggagttgctcca
ataactaatgattcggaaggaactacagttacaacaccttcggatattggaatgacctgg
ggaactagcggacatttaggaggagacgaagaaagtattggaggtgaagctgcaccagat
tggattgatgaggatgtcaatgtcgatgatgcagaaatggatttctcatcgatgtctgcc
gcatctaaggatatattcaaagatgatgatattgataatttcaatcagcaaatgaaaaga
cagagaagtccaagtcaagcttcatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGCCGTTGAGTGCGGAACAGATGG 405 491 4363 Antisense
GAAGATCTTATTCGACAAGCTTCTG 457 337 4387 Antisense
AGCTTCTGGAGTTGCTCCAATAACT 411 79 4404 Antisense
GGAACTACAGTTACAACACCTTCGG 652 529 4441 Antisense
GTTACAACACCTTCGGATATTGGAA 361 443 4450 Antisense
GAATGACCTGGGGAACTAGCGGACA 575 315 4472 Antisense
GAGGTGAAGCTGCACCAGATTGGAT 46 403 4523 Antisense
AATGGATTTCTCATCGATGTCTGCC 437 163 4578 Antisense
TCTCATCGATGTCTGCCGCATCTAA 268 613 4586 Antisense
GTCTGCCGCATCTAAGGATATATTC 192 471 4596 Antisense
GAGAAGTCCAAGTCAAGCTTCATCA 437 391 4665 Antisense
Sequence Source Affymetrix Proprietary Database