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[179932_at]

Description W10C8.1 /REP_DB=WormBase Gene ID /WP=CE25155 /TR=O45009 /GB=AAK21500.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 23      6grp_cgc > 41      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 70.8
(2.3)
P 		9.6
(-2.8)
A 		6.7
(-3)
A 		79.7
(3)
A 		73
B 95.2
(3)
A 		67.2
(0.4)
A 		30.5
(-3)
A 		62
(-0.1)
A 		65
C 63.6
(2.2)
P 		9.1
(-3)
A 		52.2
(1.1)
A 		71.6
(3)
A 		63
avr 76.5
(3) 		28.6
(-3) 		29.8
(-2.8) 		71.1
(2.3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 102.3
(3)
P 		74.5
(-0.3)
A 		66
(-1.4)
A 		95.4
(2.2)
A 		52.5
(-3)
P 		57.3
(-2.4)
A 		50
B 56.7
(3)
P 		54.5
(2.7)
A 		43.2
(0.9)
A 		49.2
(1.8)
A 		37.3
(-0)
A 		18.5
(-3)
A 		38
C 71.8
(2.5)
A 		67.5
(2.2)
A 		48.9
(0.6)
A 		11.5
(-2.5)
A 		77.2
(3)
P 		5.8
(-3)
A 		71
avr 76.9
(3) 		65.5
(1.6) 		52.7
(0.1) 		52
(-0) 		55.7
(0.4) 		27.2
(-3) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 25.8
(3)
P 		2.1
(-2.9)
A 		1.7
(-3)
A 		11.7
(-0.5)
A 		24
B 30.4
(3)
A 		16.6
(-0.6)
A 		7.1
(-3)
A 		13.2
(-1.4)
A 		23
C 21.1
(3)
P 		2.6
(-3)
A 		18.2
(2.1)
A 		12.1
(0.1)
A 		19
avr 25.8
(3) 		7.1
(-3) 		9
(-2.4) 		12.3
(-1.3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 18.8
(-0.7)
P 		26.5
(3)
A 		14.4
(-2.8)
A 		24.2
(1.9)
A 		15.8
(-2.1)
P 		13.9
(-3)
A 		13
B 19.7
(3)
P 		18.2
(2.3)
A 		10.5
(-1.3)
A 		13
(-0.1)
A 		15.2
(0.9)
A 		6.9
(-3)
A 		13
C 26.6
(3)
A 		20.6
(1.5)
A 		12.4
(-0.4)
A 		2.3
(-2.9)
A 		25
(2.6)
P 		1.9
(-3)
A 		25
avr 21.7
(3) 		21.8
(3) 		12.4
(-1) 		13.2
(-0.6) 		18.7
(1.7) 		7.6
(-3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179932_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.217
Sequence Type Exemplar sequence
Representative Public ID W10C8.1 NCBI
Target Description W10C8.1 /REP_DB=WormBase Gene ID /WP=CE25155 /TR=O45009 /GB=AAK21500.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058654(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058654 NCBI Caenorhabditis elegans Calcium Channel, Beta subunit family member (ccb-2) (ccb-2) mRNA, complete cds. 11/11 None
SNAP00000012334 ENSEMBL cdna:SNAP chromosome:CEL140:I:2840091:2849793:1 11/11 None
GENEFINDER00000012339 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2846721:2849793:1 11/11 None
W10C8.1 ENSEMBL cdna:known chromosome:CEL140:I:2846721:2849793:1 gene:W10C8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2846720-2849793(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ccb-2
Ensembl W10C8.1
Entrez Gene 189330 Entrez gene
SwissProt O45009 EMBL-EBI
WormBase CE25155 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6816 calcium ion transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5245 voltage-gated calcium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491055 Calcium Channel, Beta subunit family member (ccb-2) [Caenorhabditis elegans] gb|AAK21500.1| Calcium channel, beta subunit protein 2 [Caenorhabditis elegans] 0.0
blast CAE60598 Hypothetical protein CBG04234 [Caenorhabditis briggsae] 0.0
blast H87731 protein W10C8.1 [imported] - Caenorhabditis elegans 1.0E-178
blast NP_491055 Calcium Channel, Beta subunit family member (ccb-2) [Caenorhabditis elegans] gb|AAK21500.1| Calcium channel, beta subunit protein 2 [Caenorhabditis elegans] 1.0E-151
Protein Domains
Database ID Description E-Value
Pfam IPR000584 EMBL-EBI Dihydropyridine sensitive L-type calcium channel, beta subunit 8.2E-6
Pfam IPR000584 EMBL-EBI Dihydropyridine sensitive L-type calcium channel, beta subunit 3.1E-5
Pfam IPR000584 EMBL-EBI Dihydropyridine sensitive L-type calcium channel, beta subunit 8.2E-6
Pfam IPR000584 EMBL-EBI Dihydropyridine sensitive L-type calcium channel, beta subunit 3.1E-5
Sequence
Target Sequence 
>C. ELEGANS:179932_AT
agtggtaccttcaagtcggccaattgtacttctaggtccaactctacaacattcaaggct
cactcagttattgcatttggcgctgagagaggagattttaaagcatttcgggaaaaatat
gaagtacgtaaagtcagatatcggaagtggtcaacaatcaggagatcggaaaggagcaag
atggcttcgaggacttagagactatgatcgaggacaggatgagaatcaagaggtggaatt
gattatgagaatgacatcgaaactacacttgttactggttgactcaccacatattcatac
tcctgatgatgttcaacatttgccattatcgcctatcttttttcttatcagagtttctga
taataagattctagccaaactgctccgcaacacgggaacaacacggatggctgctgaaat
agaagtggcaaatgttctgaaaacaatgaacgacgacagagtaggtggtcacgacgatga
gcagctcatcgcgcgaggattcgaaatggtgattgaagaaaacggattacgtgaagctac
tcgacgaatcatatcctatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGGTACCTTCAAGTCGGCCAATT 253 57 474 Antisense
TCGGCCAATTGTACTTCTAGGTCCA 222 603 489 Antisense
GGTCCAACTCTACAACATTCAAGGC 148 503 508 Antisense
AAGGCTCACTCAGTTATTGCATTTG 381 161 528 Antisense
TACACTTGTTACTGGTTGACTCACC 524 641 737 Antisense
ACTCACCACATATTCATACTCCTGA 361 101 755 Antisense
TATCGCCTATCTTTTTTCTTATCAG 357 659 800 Antisense
TAAGATTCTAGCCAAACTGCTCCGC 643 637 837 Antisense
AACTGCTCCGCAACACGGGAACAAC 185 141 851 Antisense
CGGGAACAACACGGATGGCTGCTGA 13 245 866 Antisense
GCTACTCGACGAATCATATCCTATT 129 303 1009 Antisense
Sequence Source Affymetrix Proprietary Database