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[179930_at]

Description T27A1.5 /REP_DB=WormBase Gene ID /WP=CE14213 /TR=O17275 /GB=AAB71045.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 20      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 32      6grp_total > 40      4grp_total > 59      6grp_highest_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2195
1 2 3 4 range
A 2014.3
(0.9)
P 		2462.5
(2.1)
P 		2796.7
(3)
P 		604.5
(-3)
P 		2192
B 1803
(0.1)
P 		2727.4
(2.8)
P 		2799
(3)
P 		770.8
(-3)
P 		2028
C 1973.7
(0.7)
P 		2775.3
(3)
P 		2747.4
(2.9)
P 		728.3
(-3)
P 		2047
avr 1930.3
(0.5) 		2655.1
(2.6) 		2781
(3) 		701.2
(-3) 		2080

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 790
1 2 3 4 5 6 range
A 1129.8
(0.5)
P 		1438.7
(3)
P 		1272.4
(1.7)
P 		1138
(0.6)
P 		771
(-2.4)
P 		697.2
(-3)
P 		742
B 1325.3
(1.7)
P 		1340.6
(1.8)
P 		1487.5
(3)
P 		1258.2
(1.2)
P 		729.6
(-3)
P 		734.2
(-3)
P 		758
C 1303.2
(1.9)
P 		1311.4
(1.9)
P 		1386.8
(2.6)
P 		1440.6
(3)
P 		716.6
(-3)
P 		715.7
(-3)
P 		725
avr 1252.8
(1.8) 		1363.6
(2.8) 		1382.2
(3) 		1278.9
(2.1) 		739.1
(-2.8) 		715.7
(-3) 		667
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 869
1 2 3 4 range
A 733.4
(3)
P 		534.6
(1.2)
P 		731.1
(3)
P 		88.5
(-3)
P 		645
B 575
(1.8)
P 		673.6
(3)
P 		648.6
(2.7)
P 		164.3
(-3)
P 		509
C 653.4
(0.8)
P 		797.9
(1.9)
P 		957.6
(3)
P 		122.8
(-3)
P 		835
avr 653.9
(1.9) 		668.7
(2) 		779.1
(3) 		125.2
(-3) 		654

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 208.3
(-2.3)
P 		511.7
(3)
P 		277.1
(-1.1)
P 		288.6
(-0.9)
P 		232.7
(-1.9)
P 		169.8
(-3)
P 		342
B 461.1
(3)
P 		446.2
(2.5)
P 		361.2
(-0.2)
P 		333.3
(-1.1)
P 		296.4
(-2.3)
P 		273.8
(-3)
P 		187
C 483
(3)
P 		400.1
(1)
P 		353
(-0.1)
P 		289.7
(-1.6)
P 		231.9
(-3)
P 		239.6
(-2.8)
P 		251
avr 384.1
(1.2) 		452.7
(3) 		330.4
(-0.3) 		303.9
(-1) 		253.7
(-2.3) 		227.7
(-3) 		225
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179930_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5174
Sequence Type Exemplar sequence
Representative Public ID T27A1.5 NCBI
Target Description T27A1.5 /REP_DB=WormBase Gene ID /WP=CE14213 /TR=O17275 /GB=AAB71045.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061350(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061350 NCBI Caenorhabditis elegans T27A1.5 (T27A1.5) mRNA, complete cds. 11/11 None
SNAP00000021278 ENSEMBL cdna:SNAP chromosome:CEL140:II:522611:525853:1 11/11 None
GENEFINDER00000021286 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:516155:525853:1 11/11 None
T27A1.5.1 ENSEMBL cdna:known chromosome:CEL140:II:522593:525970:1 gene:T27A1.5 11/11 None
T27A1.5.2 ENSEMBL cdna:known chromosome:CEL140:II:522601:525970:1 gene:T27A1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:522610-525853(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27A1.5
Entrez Gene 173442 Entrez gene
SwissProt O17275 EMBL-EBI
WormBase CE14213 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247120_AT amino acid transporter family protein at
CANINE_2:CFAAFFX.7283.1.S1_AT similar to solute carrier family 36 (proton/amino acid symporter), member 4 cfa
DROSOPHILA_2:1623632_S_AT dm
DROSGENOME1:148544_AT dm
CHICKEN:GGA.10464.1.S1_AT solute carrier family 36 (proton/amino acid symporter), member 4 gga
CHICKEN:GGA.11234.1.S1_AT solute carrier family 36 (proton/amino acid symporter), member 4 gga
CHICKEN:GGAAFFX.11053.1.S1_AT solute carrier family 36 (proton/amino acid symporter), member 4 gga
HU35KSUBB:RC_H08077_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
HU35KSUBD:RC_T10352_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
U133_X3P:HS.292653.0.A1_3P_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U133_PLUS_2:234978_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U133B:234978_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U95B:42965_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U95B:49198_AT solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U133_PLUS_2:237096_AT Solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U133B:237096_AT Solute carrier family 36 (proton/amino acid symporter), member 4 hs
HG-U95B:48205_AT Solute carrier family 36 (proton/amino acid symporter), member 4 hs
HU35KSUBB:RC_AA113344_AT Solute carrier family 36 (proton/amino acid symporter), member 4 hs
U133_X3P:HS.148766.0.A1_3P_AT Solute carrier family 36 (proton/amino acid symporter), member 4 hs
MG-U74CV2:168438_R_AT solute carrier family 36 (proton/amino acid symporter), member 4 mm
MG-U74BV2:110443_AT Proton/amino acid transporter 4 (PAT4) mm
MOE430B:1455249_AT Proton/amino acid transporter 4 (PAT4) mm
MOUSE430_2:1455249_AT Proton/amino acid transporter 4 (PAT4) mm
RAE230B:1398531_AT Solute carrier family 36 (proton/amino acid symporter), member 4 (predicted) rn
RAT230_2:1398531_AT Solute carrier family 36 (proton/amino acid symporter), member 4 (predicted) rn
RG-U34B:RC_AI058947_AT Solute carrier family 36 (proton/amino acid symporter), member 4 (predicted) rn
YEAST_2:1772047_AT Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6865 amino acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5279 amino acid-polyamine transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_493751 T27A1.5 [Caenorhabditis elegans] gb|AAB71045.1| Hypothetical protein T27A1.5 [Caenorhabditis elegans] 0.0
blast CAE62853 Hypothetical protein CBG07032 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Pfam IPR001699 EMBL-EBI Transcription factor, T-box 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493751.1 10 70-92,133-152,167-186,193-215,235-257,270-292,312-334,355-373,377-399,419-441
T27A1.5.1 10 70-92,133-152,167-186,193-215,235-257,270-292,312-334,355-373,377-399,419-441
T27A1.5.2 10 70-92,133-152,167-186,193-215,235-257,270-292,312-334,355-373,377-399,419-441
SNAP00000021278 10 70-92,133-152,167-186,193-215,235-257,270-292,312-334,355-373,377-399,419-441
GENEFINDER00000021286 10 417-439,480-499,514-533,540-562,582-604,617-639,659-681,702-720,724-746,766-788
Sequence
Target Sequence 
>C. ELEGANS:179930_AT
aaggagtcgctgtagtacttccaattgaaaatcaaatgaatgaaccaattcactttatta
ccccaaatggagtacttaatacttcctgtattttagtacttttggtctatatgactgttg
gattctttggatttttgagatatggaaatgatattaaggatacgttgacacttaatcttc
cacagactccattctaccaagccatcaaagttatgttcgttctttgcattttggtgtcct
acccacttcaattttatgttccgatggaaagagttgaaaaatggatcaagcggaaggtgg
tagaagccaagcaggagccaatgatctacgctattcgatttggaggagttttactgacat
gcgcaatggctcaattaatcccccaccttgccctattcatctccttagtcggaagtgtcg
ctggaacatccctaaccctcgtctttccaccactcattgagcttcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGAGTCGCTGTAGTACTTCCAAT 449 159 752 Antisense
TGAACCAATTCACTTTATTACCCCA 521 573 792 Antisense
ACACTTAATCTTCCACAGACTCCAT 306 107 919 Antisense
TCCATTCTACCAAGCCATCAAAGTT 54 591 939 Antisense
CGTTCTTTGCATTTTGGTGTCCTAC 354 247 969 Antisense
TGTCCTACCCACTTCAATTTTATGT 303 557 986 Antisense
GAGCCAATGATCTACGCTATTCGAT 202 385 1066 Antisense
AGGAGTTTTACTGACATGCGCAATG 59 55 1095 Antisense
ATGCGCAATGGCTCAATTAATCCCC 377 41 1110 Antisense
CCCTATTCATCTCCTTAGTCGGAAG 512 257 1142 Antisense
CTTTCCACCACTCATTGAGCTTCTA 579 223 1194 Antisense
Sequence Source Affymetrix Proprietary Database