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[179913_at]

Description E04F6.9 /REP_DB=WormBase Gene ID /WP=CE01221 /TR=Q19064 /GB=AAA68364.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 83      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 39      6grp_total > 74      4grp_total > 03      6grp_high1_sub > 04      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9393
1 2 3 4 range
A 2296.8
(-2.3)
P 		1499.8
(-2.8)
P 		1140.5
(-3)
P 		10533.9
(3)
P 		9393
B 2315.4
(-2.2)
P 		1272.1
(-3)
P 		1243.2
(-3)
P 		9605.3
(3)
P 		8362
C 2295
(-2.1)
P 		1297.5
(-2.9)
P 		1161.6
(-3)
P 		8886.9
(3)
P 		7725
avr 2302.4
(-2.2) 		1356.5
(-2.9) 		1181.8
(-3) 		9675.4
(3) 		8494

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9919
1 2 3 4 5 6 range
A 3581.4
(-2.6)
P 		3064.2
(-3)
P 		9321
(1.5)
P 		11318.9
(3)
P 		9205.6
(1.5)
P 		10645
(2.5)
P 		8255
B 3328.7
(-2.6)
P 		2817.8
(-3)
P 		10257.6
(3)
P 		9585.6
(2.5)
P 		9567.4
(2.4)
P 		9842.3
(2.7)
P 		7440
C 3075.8
(-2.9)
P 		2947.8
(-3)
P 		9311
(0.9)
P 		12736.9
(3)
P 		9495.1
(1)
P 		10902.3
(1.9)
P 		9789
avr 3328.6
(-2.7) 		2943.3
(-3) 		9629.9
(1.9) 		11213.8
(3) 		9422.7
(1.7) 		10463.2
(2.5) 		8271
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1759
1 2 3 4 range
A 836.3
(-0.4)
P 		325.6
(-2.9)
P 		298.1
(-3)
P 		1542
(3)
P 		1244
B 738.4
(-1.5)
P 		314.2
(-2.9)
P 		288.1
(-3)
P 		2046.9
(3)
P 		1759
C 759.7
(-0.9)
P 		373
(-3)
P 		404.9
(-2.8)
P 		1498.5
(3)
P 		1126
avr 778.1
(-1) 		337.6
(-3) 		330.4
(-3) 		1695.8
(3) 		1365

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3227
1 2 3 4 5 6 range
A 660.2
(-3)
P 		1089.8
(-1.8)
P 		2029.9
(0.7)
P 		2870.7
(3)
P 		2777.8
(2.7)
P 		2591.7
(2.2)
P 		2211
B 1158.2
(-2.6)
P 		937.9
(-3)
P 		2490.9
(0.2)
P 		2538.9
(0.3)
P 		3887.1
(3)
P 		3670.2
(2.6)
P 		2949
C 1140.1
(-2.5)
P 		899.5
(-3)
P 		2369.8
(0.2)
P 		2561.4
(0.6)
P 		3072.5
(1.7)
P 		3649.6
(3)
P 		2750
avr 986.2
(-3) 		975.7
(-3) 		2296.9
(0.4) 		2657
(1.3) 		3245.8
(2.9) 		3303.8
(3) 		2328
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179913_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5549
Sequence Type Exemplar sequence
Representative Public ID E04F6.9 NCBI
Target Description E04F6.9 /REP_DB=WormBase Gene ID /WP=CE01221 /TR=Q19064 /GB=AAA68364.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063102(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063102 NCBI Caenorhabditis elegans E04F6.9 (E04F6.9) mRNA, complete cds. 11/11 None
SNAP00000032900 ENSEMBL cdna:SNAP chromosome:CEL140:II:7211611:7212087:-1 11/11 None
GENEFINDER00000032906 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7211611:7212087:-1 11/11 None
E04F6.9 ENSEMBL cdna:known chromosome:CEL140:II:7211604:7212090:-1 gene:E04F6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7211606-7212083(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E04F6.9
Entrez Gene 174185 Entrez gene
SwissProt Q19064 EMBL-EBI
WormBase CE01221 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68364 Hypothetical protein E04F6.9 [Caenorhabditis elegans] ref|NP_495503.1| E04F6.9 [Caenorhabditis elegans] 5.0E-66
blast CAE67572 Hypothetical protein CBG13100 [Caenorhabditis briggsae] 2.0E-52
blast CAE67573 Hypothetical protein CBG13102 [Caenorhabditis briggsae] 5.0E-38
Sequence
Target Sequence 
>C. ELEGANS:179913_AT
gccatctttgccatatccgatgctgccgttgctcaacagcaggttaaggacggagaaaaa
gttgaaatcgatgctttcaagggagccaaggcaatcaagagaaccgttgccggtggagat
caaatcttccaccttgacggagataacaagggatcatttgttgatgctaagggaaagaag
attgagtcaaccaattatgaagctaataacggaatccttatcattaagaagttcaccaag
gccgatgttggaacctactccgagcacccagctaaaaacaccgaaaccaagcacgctgat
ggatccatctccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCATCTTTGCCATATCCGATGCTG 486 279 49 Antisense
CGATGCTGCCGTTGCTCAACAGCAG 538 241 66 Antisense
TCGATGCTTTCAAGGGAGCCAAGGC 52 603 116 Antisense
GAGCCAAGGCAATCAAGAGAACCGT 277 385 131 Antisense
AACCGTTGCCGGTGGAGATCAAATC 610 143 150 Antisense
ATCAAATCTTCCACCTTGACGGAGA 78 25 167 Antisense
AAGAAGTTCACCAAGGCCGATGTTG 501 151 274 Antisense
CGATGTTGGAACCTACTCCGAGCAC 405 247 291 Antisense
CTCCGAGCACCCAGCTAAAAACACC 584 231 306 Antisense
AAAACACCGAAACCAAGCACGCTGA 522 127 323 Antisense
AAGCACGCTGATGGATCCATCTCCG 591 151 337 Antisense
Sequence Source Affymetrix Proprietary Database