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[179911_at]

Description T12B5.8 /REP_DB=WormBase Gene ID /WP=CE20066 /TR=Q9TZ27 /GB=AAC68933.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_high_8_8 > 33      6grp_highest_nonorm > 81      6grp_total > 62      4grp_total > 53      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 168.6
(0.3)
P 		187.3
(3)
P 		145.7
(-3)
P 		157.8
(-1.3)
P 		42
B 136.5
(3)
P 		129
(2.3)
P 		128.2
(2.3)
P 		69.6
(-3)
M 		67
C 171.8
(3)
P 		111.2
(-3)
P 		134.5
(-0.7)
P 		123.9
(-1.7)
P 		61
avr 159
(3) 		142.5
(0.6) 		136.1
(-0.3) 		117.1
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 106.6
(-3)
P 		227.4
(0.3)
P 		328.6
(3)
P 		306.5
(2.4)
P 		175.3
(-1.1)
P 		130.7
(-2.3)
P 		222
B 137.8
(-2.3)
P 		253.8
(1.5)
P 		299.4
(3)
P 		288
(2.6)
P 		275.6
(2.2)
P 		115.6
(-3)
P 		184
C 118
(-3)
P 		243.6
(0.8)
P 		315.7
(3)
P 		285.8
(2.1)
P 		226.1
(0.3)
P 		129.9
(-2.6)
P 		198
avr 120.8
(-3) 		241.6
(0.7) 		314.6
(3) 		293.4
(2.3) 		225.7
(0.2) 		125.4
(-2.9) 		194
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 61.4
(3)
P 		40.7
(-0.2)
P 		38.1
(-0.7)
P 		23.1
(-3)
P 		38
B 43.5
(3)
P 		31.9
(0.6)
P 		29.7
(0.1)
P 		14.8
(-3)
M 		29
C 56.9
(3)
P 		32
(-1.2)
P 		46.9
(1.3)
P 		20.9
(-3)
P 		36
avr 53.9
(3) 		34.9
(-0.3) 		38.2
(0.3) 		19.6
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 19.6
(-3)
P 		80.9
(3)
P 		71.6
(2.1)
P 		77.7
(2.7)
P 		52.9
(0.3)
P 		31.8
(-1.8)
P 		61
B 47.9
(-2.6)
P 		84.5
(0.6)
P 		72.7
(-0.4)
P 		76.3
(-0.1)
P 		112
(3)
P 		43.1
(-3)
P 		69
C 43.7
(-3)
P 		74.3
(2)
P 		80.4
(3)
P 		57.5
(-0.7)
P 		73.2
(1.8)
P 		43.5
(-3)
P 		37
avr 37.1
(-3) 		79.9
(3) 		74.9
(2.3) 		70.5
(1.7) 		79.4
(2.9) 		39.5
(-2.7) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179911_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8230
Sequence Type Exemplar sequence
Representative Public ID T12B5.8 NCBI
Target Description T12B5.8 /REP_DB=WormBase Gene ID /WP=CE20066 /TR=Q9TZ27 /GB=AAC68933.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064898
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
T12B5.8 ENSEMBL cdna:known chromosome:CEL140:III:940866:943445:-1 gene:T12B5.8 None
GENEFINDER00000007715 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:940866:943445:-1 None
NM_064898 NCBI Caenorhabditis elegans T12B5.8 (T12B5.8) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:940005-943445(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.8
Entrez Gene 188447 Entrez gene
SwissProt Q9TZ27 EMBL-EBI
WormBase CE32934 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68933 Hypothetical protein T12B5.8 [Caenorhabditis elegans] ref|NP_497299.2| T12B5.8 [Caenorhabditis elegans] 0.0
blast AAC68933 Hypothetical protein T12B5.8 [Caenorhabditis elegans] ref|NP_497299.2| T12B5.8 [Caenorhabditis elegans] 1.0E-145
blast AAC68934 Hypothetical protein T12B5.10 [Caenorhabditis elegans] ref|NP_497300.1| T12B5.10 [Caenorhabditis elegans] 1.0E-124
blast AAC68752 Hypothetical protein C29F9.11 [Caenorhabditis elegans] ref|NP_497143.1| C29F9.11 [Caenorhabditis elegans] 1.0E-109
blast AAC68752 Hypothetical protein C29F9.11 [Caenorhabditis elegans] ref|NP_497143.1| C29F9.11 [Caenorhabditis elegans] 1.0E-108
blast AAC68934 Hypothetical protein T12B5.10 [Caenorhabditis elegans] ref|NP_497300.1| T12B5.10 [Caenorhabditis elegans] 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.9E-61
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.7E-10
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.9E-61
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.3E-4
Sequence
Target Sequence 
>C. ELEGANS:179911_AT
gattgttctgagattgccagtacaatgattgttcatttttttcatttcaaatgtttcact
attaaccgcactgacgaatttccagcacaggctgcaatacaaatgagagatgatctttta
agaagaagtacatttcaaagttgcgatatatctttttacaaatcaaagacaaatccgatt
gaaattgcaaaagtattcaaaccggactacactggcgaaaacgtatttaagattgaattt
tcgaatggtaacgacaaattcgatatttcgctccaagaggtttcagactatttttatttg
aatatgaaaagatccctttcagatcttttgagaaggagtacatttcaaagctgcttgatt
tattttgaaaaatcaaagacaaagacaaatccgattgaaattgcaaaagtattcaagcca
gactataccggcagcaatgaatatgagattgaattttcaaatgataacgataaattcaat
atttcgtttcgacatttacctgtacataaaaaaatctatttcttactcgaggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGTTCTGAGATTGCCAGTACAA 79 435 637 Antisense
AATGTTTCACTATTAACCGCACTGA 391 163 686 Antisense
ACTATTAACCGCACTGACGAATTTC 683 99 694 Antisense
GTTGCGATATATCTTTTTACAAATC 385 439 776 Antisense
GATATTTCGCTCCAAGAGGTTTCAG 218 427 898 Antisense
AGAGGTTTCAGACTATTTTTATTTG 572 45 912 Antisense
GATCCCTTTCAGATCTTTTGAGAAG 246 417 947 Antisense
GAGTACATTTCAAAGCTGCTTGATT 644 399 972 Antisense
CAAGCCAGACTATACCGGCAGCAAT 291 185 1050 Antisense
TTCGTTTCGACATTTACCTGTACAT 285 695 1119 Antisense
AAAAATCTATTTCTTACTCGAGGTG 679 127 1146 Antisense
Sequence Source Affymetrix Proprietary Database