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[179883_at]

Description F58H7.3 /REP_DB=WormBase Gene ID /WP=CE17932 /TR=O61216 /GB=AAC02597.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 38      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 278.4
(3)
A 		90.8
(-1.4)
A 		22.6
(-3)
A 		61.3
(-2.1)
A 		256
B 153.3
(3)
A 		139.3
(2.1)
A 		62.3
(-3)
A 		116
(0.5)
A 		91
C 230.5
(3)
A 		116.2
(-2)
A 		93.1
(-3)
A 		162.1
(0)
A 		137
avr 220.7
(3) 		115.4
(-0.9) 		59.3
(-3) 		113.1
(-1) 		161

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 177.3
(1.2)
A 		240.6
(3)
A 		181.7
(1.3)
A 		110
(-0.7)
A 		131.4
(-0.1)
A 		27.6
(-3)
A 		213
B 220.1
(3)
A 		172.5
(1.4)
M 		81.1
(-1.8)
A 		100.4
(-1.1)
A 		96.8
(-1.2)
A 		45.5
(-3)
A 		175
C 194.3
(2.3)
A 		214.1
(3)
A 		91.2
(-1.3)
A 		150.3
(0.7)
A 		94.6
(-1.2)
A 		44.2
(-3)
A 		170
avr 197.2
(2.6) 		209.1
(3) 		118
(-0.2) 		120.2
(-0.1) 		107.6
(-0.6) 		39.1
(-3) 		170
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 101.4
(3)
A 		19.7
(-2.1)
A 		5.9
(-3)
A 		9
(-2.8)
A 		96
B 48.9
(3)
A 		34.4
(0.5)
A 		14.4
(-3)
A 		24.7
(-1.2)
A 		35
C 76.3
(3)
A 		33.4
(-2.3)
A 		32.5
(-2.4)
A 		27.3
(-3)
A 		49
avr 75.5
(3) 		29.2
(-1.8) 		17.6
(-3) 		20.3
(-2.7) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 32.7
(-1)
A 		85.6
(3)
A 		39.6
(-0.5)
A 		27.9
(-1.4)
A 		39.7
(-0.5)
A 		6.7
(-3)
A 		79
B 76.6
(3)
A 		57.4
(1.1)
M 		19.7
(-2.7)
A 		26.6
(-2)
A 		39.3
(-0.8)
A 		17
(-3)
A 		60
C 72
(3)
A 		65.3
(2.3)
A 		23.2
(-2.1)
A 		30.2
(-1.4)
A 		30.6
(-1.3)
A 		14.8
(-3)
A 		57
avr 60.4
(2) 		69.4
(3) 		27.5
(-1.4) 		28.2
(-1.4) 		36.5
(-0.5) 		12.8
(-3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179883_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10692
Sequence Type Exemplar sequence
Representative Public ID F58H7.3 NCBI
Target Description F58H7.3 /REP_DB=WormBase Gene ID /WP=CE17932 /TR=O61216 /GB=AAC02597.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067614
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067614 NCBI Caenorhabditis elegans F58H7.3 (F58H7.3) mRNA, complete cds. None
F58H7.3 ENSEMBL cdna:known chromosome:CEL140:IV:924936:939562:1 gene:F58H7.3 None
SNAP00000022284 ENSEMBL cdna:SNAP chromosome:CEL140:IV:924936:939555:1 None
GENEFINDER00000022285 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:936642:939562:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:924941-939568(+) 94.36 100.0
Public Domain and Genome References
Gene Title
Ensembl F58H7.3
Entrez Gene 186551 Entrez gene
SwissProt O61216 EMBL-EBI
WormBase CE34325 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC02597 Hypothetical protein F58H7.3 [Caenorhabditis elegans] ref|NP_500015.2| F58H7.3 [Caenorhabditis elegans] 0.0
blast CAE68290 Hypothetical protein CBG13975 [Caenorhabditis briggsae] 0.0
blast AAC02597 Hypothetical protein F58H7.3 [Caenorhabditis elegans] ref|NP_500015.2| F58H7.3 [Caenorhabditis elegans] 1.0E-169
blast CAE68290 Hypothetical protein CBG13975 [Caenorhabditis briggsae] 1.0E-114
blast CAB03855 Hypothetical protein F21A3.7 [Caenorhabditis elegans] emb|CAB04158.2| Hypothetical protein F21A3.7 [Caenorhabditis elegans] ref|NP_506861.2| F21A3.7 [Caenorhabditis elegans] 4.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 3.2E-54
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 6.5E-20
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.7E-9
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 6.2E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500015.2 4 297-319,363-382,406-428,560-582
F58H7.3 4 297-319,363-382,406-428,560-582
SNAP00000022284 4 302-324,337-356,380-402,534-553
GENEFINDER00000022285 4 67-89,102-121,145-167,299-321
Sequence
Target Sequence 
>C. ELEGANS:179883_AT
tgtgcccgggcagcgtgttgaaaataataattctccggtcaatgcaccatgtgcaccaaa
cttcatttttaacagtatttatttgaaaaaaaaattttcttattggaaattttttaataa
aactgaagcaaaccaacaagatttttttattcagagtgtcccatggtacatccgatggtt
ggctgcaaagaagctcttctgctgttacgttccaaaatctttccgttatgataaagatgc
atacagcagaaataataatgttcaagcaatatcattacattcaacacctgatagaaatgt
tgcaaagaatttgatacccaaagagataaacttcaccgaagtcaaccaagatgctccatt
gcacgatgtactggaaggcccttccgccgagaaaacgcaaaaaatcgatttaatcgtctc
gttactccgagaaataatccatctgaaagaaagaattggagcatccggacaattggctcc
atattgggatcaaattatatctcgctgcgaaaaagtttcacttagtttttatctattcct
tatcactat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGCCCGGGCAGCGTGTTGAAAAT 524 557 1374 Antisense
AATTCTCCGGTCAATGCACCATGTG 486 177 1402 Antisense
TGCACCATGTGCACCAAACTTCATT 439 577 1416 Antisense
TCAGAGTGTCCCATGGTACATCCGA 185 623 1524 Antisense
AAGAAGCTCTTCTGCTGTTACGTTC 308 153 1561 Antisense
ACCGAAGTCAACCAAGATGCTCCAT 34 89 1708 Antisense
GATGCTCCATTGCACGATGTACTGG 74 413 1723 Antisense
GATGTACTGGAAGGCCCTTCCGCCG 380 409 1738 Antisense
TCGATTTAATCGTCTCGTTACTCCG 15 605 1778 Antisense
GCATCCGGACAATTGGCTCCATATT 266 309 1834 Antisense
GTTTTTATCTATTCCTTATCACTAT 477 449 1898 Antisense
Sequence Source Affymetrix Proprietary Database