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[179876_at]

Description ZK430.5 /REP_DB=WormBase Gene ID /WP=CE05081 /TR=Q23491 /GB=AAA83576.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 11      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 20.7
(-3)
A 		42.7
(-1)
P 		29.3
(-2.2)
A 		87.2
(3)
P 		67
B 27.5
(1.4)
M 		3.6
(-3)
A 		4
(-2.9)
A 		36.1
(3)
A 		33
C 30.8
(-1.5)
A 		31.9
(-1.4)
A 		3.8
(-3)
A 		110.4
(3)
P 		107
avr 26.3
(-1.7) 		26.1
(-1.7) 		12.4
(-3) 		77.9
(3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 31.4
(2.6)
A 		16.8
(-0.5)
A 		20.2
(0.2)
A 		5.3
(-3)
A 		33.1
(3)
A 		24.2
(1.1)
A 		28
B 14.5
(-1.3)
A 		2.7
(-3)
A 		41.9
(2.8)
A 		22.6
(-0.1)
A 		12.6
(-1.5)
A 		43.5
(3)
A 		41
C 26.9
(0.4)
A 		2.9
(-3)
A 		24.5
(0.1)
A 		4.2
(-2.8)
A 		7.1
(-2.4)
A 		44.9
(3)
P 		42
avr 24.3
(0.4) 		7.5
(-3) 		28.9
(1.3) 		10.7
(-2.4) 		17.6
(-1) 		37.5
(3) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 7.6
(-3)
A 		9.3
(-1)
P 		7.6
(-3)
A 		12.8
(3)
P 		5
B 8.8
(3)
M 		0.9
(-3)
A 		0.9
(-3)
A 		7.7
(2.2)
A 		8
C 10.2
(0.1)
A 		9.2
(-0.3)
A 		1.3
(-3)
A 		18.6
(3)
P 		17
avr 8.9
(0.5) 		6.5
(-1) 		3.3
(-3) 		13
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 5.8
(0.1)
A 		6
(0.2)
A 		4.4
(-0.9)
A 		1.3
(-3)
A 		10
(3)
A 		5.9
(0.2)
A 		9
B 5
(-1.4)
A 		0.9
(-3)
A 		10.2
(0.6)
A 		6
(-1)
A 		5.1
(-1.4)
A 		16.2
(3)
A 		15
C 10
(0.9)
A 		0.9
(-3)
A 		6.2
(-0.7)
A 		0.8
(-3)
A 		2.3
(-2.4)
A 		15
(3)
P 		14
avr 6.9
(-0.4) 		2.6
(-3) 		6.9
(-0.4) 		2.7
(-2.9) 		5.8
(-1) 		12.4
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179876_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4570
Sequence Type Exemplar sequence
Representative Public ID ZK430.5 NCBI
Target Description ZK430.5 /REP_DB=WormBase Gene ID /WP=CE05081 /TR=Q23491 /GB=AAA83576.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062377(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062377 NCBI Caenorhabditis elegans ZK430.5 (ZK430.5) mRNA, complete cds. 11/11 None
GENEFINDER00000014035 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4405284:4416543:1 10/11 None
ZK430.5 ENSEMBL cdna:known chromosome:CEL140:II:4405854:4416543:1 gene:ZK430.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014024 3/11 Cross Hyb Matching Probes None
SNAP00000014025 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4405852-4416542(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK430.5
Entrez Gene 191319 Entrez gene
SwissProt Q23491 EMBL-EBI
WormBase CE05081 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8233 peptidase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83576 Hypothetical protein ZK430.5 [Caenorhabditis elegans] ref|NP_494778.1| ZK430.5 [Caenorhabditis elegans] 0.0
blast NP_491160 SEParase family member (sep-1) [Caenorhabditis elegans] gb|AAF60651.1| Separase protein 1 [Caenorhabditis elegans] gb|AAK77200.1| separase [Caenorhabditis elegans] 0.0
blast CAE68974 Hypothetical protein CBG14956 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005314 EMBL-EBI Peptidase C50, separase 1.0E-126
Pfam IPR005314 EMBL-EBI Peptidase C50, separase 2.9E-46
Pfam IPR005314 EMBL-EBI Peptidase C50, separase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000014035 1 148-170
Sequence
Target Sequence 
>C. ELEGANS:179876_AT
ttccgcaagcccacggctttgatggaacatctgtgattcaggattacgcgatggcaaaat
gtccatcgcttgttggatgcttatgggatgtacaggttgtcgcaaaacacacttcaatcg
ggaataacggcaaggaaaacttacctataactacaggcagaatgatttcttattcaaaaa
aaccgaagataagagaaactgagccgaattcttcgaatcttatccttgcatcaaattata
ataatatcacttcaaaggcttcactgagtcaagaaccaattaagttgaaggaaacaacga
aaattcaaaaggaaactgtgtccaagactccgaagcagaaatctctagcttcaaatcaaa
ttcttaccttgcgttctgaaactatgaatcaagaggttcccagaaaagccataacagaga
cacggagcaaagttcagaaggcacctccgcatcctacagcctctcaaaatgaagaggaaa
aagacaatttgaaaagaacaagaaaaagaaaactgcttgtttcaaccattcctaatttct
gcaaagaacatggagcgaagaaacctagaactactcgaccgagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCGCAAGCCCACGGCTTTGATGG 513 697 3218 Antisense
GTGATTCAGGATTACGCGATGGCAA 449 485 3250 Antisense
AATGTCCATCGCTTGTTGGATGCTT 601 163 3275 Antisense
GGTTGTCGCAAAACACACTTCAATC 508 507 3312 Antisense
TCTTCGAATCTTATCCTTGCATCAA 346 617 3427 Antisense
ATCACTTCAAAGGCTTCACTGAGTC 645 27 3463 Antisense
GAAACTGTGTCCAAGACTCCGAAGC 688 357 3529 Antisense
ATCAAATTCTTACCTTGCGTTCTGA 130 27 3572 Antisense
GCATCCTACAGCCTCTCAAAATGAA 32 311 3666 Antisense
AAACTGCTTGTTTCAACCATTCCTA 599 127 3727 Antisense
GAAACCTAGAACTACTCGACCGAGT 271 355 3777 Antisense
Sequence Source Affymetrix Proprietary Database