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[179871_at]

Description T12B5.2 /REP_DB=WormBase Gene ID /WP=CE20060 /TR=Q9TZ21 /GB=AAC68928.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 42      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 81      6grp_total > 30      4grp_total > 55      6grp_highest_sub > 03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 231
1 2 3 4 range
A 243.2
(3)
P 		86.4
(-2.3)
P 		77.2
(-2.6)
P 		66.3
(-3)
P 		177
B 237.1
(3)
P 		106.5
(-1.4)
P 		66
(-2.8)
P 		59.6
(-3)
P 		178
C 290.2
(3)
P 		102.5
(-1.9)
P 		113
(-1.7)
P 		61.8
(-3)
P 		228
avr 256.8
(3) 		98.5
(-1.9) 		85.4
(-2.3) 		62.6
(-3) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 431
1 2 3 4 5 6 range
A 137.6
(-2.1)
P 		268
(-0.3)
P 		501.2
(3)
P 		360.5
(1)
P 		173.1
(-1.6)
P 		70.7
(-3)
P 		431
B 166
(-1.5)
P 		352.1
(2.8)
P 		346.2
(2.6)
P 		362
(3)
P 		288
(1.3)
P 		100.7
(-3)
P 		261
C 158.3
(-1.6)
P 		392.1
(3)
P 		311.6
(1.4)
P 		274
(0.7)
P 		274
(0.7)
P 		89.9
(-3)
P 		302
avr 154
(-1.7) 		337.4
(2) 		386.3
(3) 		332.2
(1.9) 		245
(0.2) 		87.1
(-3) 		299
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 88.6
(3)
P 		18.8
(-2.3)
P 		20.2
(-2.2)
P 		9.7
(-3)
P 		79
B 75.6
(3)
P 		26.3
(-1.7)
P 		15.3
(-2.8)
P 		12.7
(-3)
P 		63
C 96
(3)
P 		29.5
(-1.7)
P 		39.4
(-1)
P 		10.4
(-3)
P 		86
avr 86.7
(3) 		24.9
(-1.9) 		25
(-1.9) 		10.9
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 25.4
(-2.5)
P 		95.3
(2.1)
P 		109.2
(3)
P 		91.4
(1.8)
P 		52.2
(-0.7)
P 		17.2
(-3)
P 		92
B 57.8
(-1.5)
P 		117.2
(3)
P 		84.1
(0.5)
P 		95.9
(1.4)
P 		117
(3)
P 		37.5
(-3)
P 		80
C 58.7
(-1.1)
P 		119.6
(3)
P 		79.3
(0.3)
P 		55.1
(-1.3)
P 		88.7
(0.9)
P 		30.1
(-3)
P 		90
avr 47.3
(-1.6) 		110.7
(3) 		90.9
(1.6) 		80.8
(0.8) 		86
(1.2) 		28.3
(-3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179871_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8304
Sequence Type Exemplar sequence
Representative Public ID T12B5.2 NCBI
Target Description T12B5.2 /REP_DB=WormBase Gene ID /WP=CE20060 /TR=Q9TZ21 /GB=AAC68928.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064904(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064904 NCBI Caenorhabditis elegans T12B5.2 (T12B5.2) mRNA, complete cds. 11/11 None
SNAP00000007704 ENSEMBL cdna:SNAP chromosome:CEL140:III:956344:957782:1 11/11 None
GENEFINDER00000007708 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:956344:957782:1 11/11 None
T12B5.2 ENSEMBL cdna:known chromosome:CEL140:III:956321:957782:1 gene:T12B5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:956343-957782(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.2
Entrez Gene 188441 Entrez gene
SwissProt Q9TZ21 EMBL-EBI
WormBase CE20060 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68928 Hypothetical protein T12B5.2 [Caenorhabditis elegans] ref|NP_497305.1| T12B5.2 [Caenorhabditis elegans] 0.0
blast AAC68928 Hypothetical protein T12B5.2 [Caenorhabditis elegans] ref|NP_497305.1| T12B5.2 [Caenorhabditis elegans] 1.0E-176
blast AAC68933 Hypothetical protein T12B5.8 [Caenorhabditis elegans] ref|NP_497299.2| T12B5.8 [Caenorhabditis elegans] 3.0E-91
blast AAC68933 Hypothetical protein T12B5.8 [Caenorhabditis elegans] ref|NP_497299.2| T12B5.8 [Caenorhabditis elegans] 3.0E-88
blast AAC68934 Hypothetical protein T12B5.10 [Caenorhabditis elegans] ref|NP_497300.1| T12B5.10 [Caenorhabditis elegans] 2.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 6.7E-58
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.3E-14
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 6.7E-58
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.5E-4
Sequence
Target Sequence 
>C. ELEGANS:179871_AT
gcgatatttttacaacgttcacagcgtttctgtgttccaaaaagtgtattctcgtggaag
aactaattctttgggacttctcgttagatgaaatcctcacaattctttcatgttttgacg
caaatgtgctcaaaaagctcgaattacaatggattgagtcagttgaccaattcaagcgaa
taactcgtttggagcattgggaaaatacagaagaactcaaaatttgggtttctaactttg
acagtgcaataattgtctattgttttcatttcaaatgctttgatattcacaaattggacg
aatttccagcacatatcgcgatacaaataagagatgatcttttgagaagcagtacatttc
atagttgtaatatatcttttgacaaatcaaagtcaaatccgattgaaattgcaagaatgt
tccaaccggactatgtcggcggcaatgaatttaaaattgaattttcaaatggtaacgaca
aatttgatatttcgctttggaactttgatgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGATATTTTTACAACGTTCACAGC 311 287 485 Antisense
CGTTCACAGCGTTTCTGTGTTCCAA 623 247 500 Antisense
TTCTTTGGGACTTCTCGTTAGATGA 224 691 551 Antisense
AAATCCTCACAATTCTTTCATGTTT 329 119 575 Antisense
GAGTCAGTTGACCAATTCAAGCGAA 700 399 640 Antisense
GGTTTCTAACTTTGACAGTGCAATA 121 505 711 Antisense
ATAATTGTCTATTGTTTTCATTTCA 71 27 733 Antisense
ATTTCCAGCACATATCGCGATACAA 496 17 786 Antisense
ATTGCAAGAATGTTCCAACCGGACT 90 3 892 Antisense
GTTCCAACCGGACTATGTCGGCGGC 230 441 903 Antisense
TATTTCGCTTTGGAACTTTGATGCA 562 661 972 Antisense
Sequence Source Affymetrix Proprietary Database