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[179869_at]

Description R12B2.5 /REP_DB=WormBase Gene ID /WP=CE25078 /TR=Q21955 /GB=AAA50743.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 58      4grp_high_8_8 > 34      6grp_highest_nonorm > 51      6grp_total > 26      4grp_total > 46      6grp_highest_sub > Cu      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 656
1 2 3 4 range
A 1192.7
(3)
P 		633
(-2.1)
P 		589.9
(-2.5)
P 		538.2
(-3)
P 		655
B 1117.4
(3)
P 		668.6
(-1.8)
P 		560.8
(-3)
P 		751.2
(-0.9)
P 		557
C 1038.6
(3)
P 		981.7
(2.3)
P 		536.5
(-3)
P 		553.1
(-2.8)
P 		502
avr 1116.2
(3) 		761.1
(-0.8) 		562.4
(-3) 		614.2
(-2.4) 		554

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 417
1 2 3 4 5 6 range
A 668.5
(3)
P 		647.5
(2.4)
P 		459
(-2.7)
P 		505.4
(-1.4)
P 		447.1
(-3)
P 		590.8
(0.9)
P 		221
B 640.4
(1.7)
P 		693
(3)
P 		579.5
(0.2)
P 		453.5
(-2.9)
P 		447.5
(-3)
P 		530.1
(-1)
P 		246
C 814
(3)
P 		674.9
(1)
P 		663.6
(0.8)
P 		397
(-3)
P 		472.7
(-1.9)
P 		489.6
(-1.7)
P 		417
avr 707.6
(3) 		671.8
(2.2) 		567.4
(-0.3) 		452
(-3) 		455.8
(-2.9) 		536.8
(-1) 		256
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 434.2
(3)
P 		137.4
(-2)
P 		154.2
(-1.7)
P 		78.8
(-3)
P 		355
B 356.3
(3)
P 		165.1
(-2.1)
P 		130
(-3)
P 		160.1
(-2.2)
P 		226
C 343.8
(3)
P 		282.2
(1.5)
P 		187
(-0.8)
P 		93.3
(-3)
P 		251
avr 378.1
(3) 		194.9
(-1.1) 		157.1
(-2) 		110.7
(-3) 		267

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 123.2
(-1.9)
P 		230.3
(3)
P 		100
(-3)
P 		128.2
(-1.7)
P 		134.9
(-1.4)
P 		143.8
(-1)
P 		130
B 222.8
(2.6)
P 		230.7
(3)
P 		140.7
(-1.9)
P 		120.1
(-3)
P 		181.8
(0.3)
P 		197.7
(1.2)
P 		111
C 301.7
(3)
P 		205.9
(0.4)
P 		168.9
(-0.6)
P 		79.8
(-3)
P 		152.9
(-1)
P 		163.9
(-0.7)
P 		222
avr 215.9
(2.7) 		222.3
(3) 		136.5
(-1.6) 		109.4
(-3) 		156.5
(-0.5) 		168.5
(0.1) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179869_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7091
Sequence Type Exemplar sequence
Representative Public ID R12B2.5 NCBI
Target Description R12B2.5 /REP_DB=WormBase Gene ID /WP=CE25078 /TR=Q21955 /GB=AAA50743.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171134(11), NM_171867(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171134 NCBI Caenorhabditis elegans MeDiaTor family member (mdt-15) (mdt-15) mRNA, complete cds. 11/11 None
NM_171867 NCBI Caenorhabditis elegans MeDiaTor family member (mdt-15) (mdt-15) mRNA, complete cds. 11/11 None
SNAP00000015244 ENSEMBL cdna:SNAP chromosome:CEL140:III:5830172:5833287:-1 11/11 None
GENEFINDER00000015250 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5830172:5835706:-1 11/11 None
R12B2.5b.1 ENSEMBL cdna:known chromosome:CEL140:III:5828509:5833695:-1 gene:R12B2.5 11/11 None
R12B2.5b.2 ENSEMBL cdna:known chromosome:CEL140:III:5828509:5833658:-1 gene:R12B2.5 11/11 None
R12B2.5a ENSEMBL cdna:known chromosome:CEL140:III:5830172:5833695:-1 gene:R12B2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5830170-5833286(-) 98.16 100.0
Public Domain and Genome References
Ensembl R12B2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM54164 Mediator protein 15, isoform b [Caenorhabditis elegans] ref|NP_741151.1| MeDiaTor family member (mdt-15) [Caenorhabditis elegans] 0.0
blast AAA50743 Mediator protein 15, isoform a [Caenorhabditis elegans] ref|NP_741152.1| MeDiaTor family member (mdt-15) [Caenorhabditis elegans] sp|Q21955|YNL5_CAEEL Hypothetical protein R12B2.5 in chromosome III 0.0
blast T16726 hypothetical protein R12B2.5 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:179869_AT
tgtcaggtccaagttctggagccccttcaatgaatccaatgggtacaccgaatcaaaaaa
tgggaacacctggatcaatgggtggaatgtcaggacttgatgatctgaactatgatgatt
tccttccaaacccaacgccgattgatgcacttcagccaacattgcacgtcgggcagaatt
cgatgaatgctggtccaccagttcaacgatcaaatctcaatgaaacagcaagaaaggaac
ttcaaatccttgatgctcgattcgagattgatccaaaccatcaacgtcatgatgccaatc
atatcattgtcgtctgtaaaatccgtaatcaacagttcccaccacttcgactcgttgttc
caaccacttatccagctggaaatgtgactgtagatcgagcagtcattgatttggatgcat
atttgtatgatgatctacaaaattcagtatatgagagattatcacgacccggtctttcgt
caattactgactatttgaatgcttgggaagaacaagtgaatcaataccaaaaccagacaa
gcggtggtctagacgtcgctttcaacgtgggcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCAGGTCCAAGTTCTGGAGCCCC 614 557 1742 Antisense
GGAGCCCCTTCAATGAATCCAATGG 272 521 1759 Antisense
TCCAAACCCAACGCCGATTGATGCA 706 585 1866 Antisense
GATTGATGCACTTCAGCCAACATTG 125 435 1881 Antisense
GCCAACATTGCACGTCGGGCAGAAT 394 277 1896 Antisense
ATTCGATGAATGCTGGTCCACCAGT 514 9 1919 Antisense
GGTCCACCAGTTCAACGATCAAATC 201 501 1933 Antisense
CTCGTTGTTCCAACCACTTATCCAG 679 229 2092 Antisense
TTATCACGACCCGGTCTTTCGTCAA 40 673 2200 Antisense
GACAAGCGGTGGTCTAGACGTCGCT 488 365 2277 Antisense
TAGACGTCGCTTTCAACGTGGGCAA 568 651 2291 Antisense
Sequence Source Affymetrix Proprietary Database