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[179866_at]

Description T12B5.3 /REP_DB=WormBase Gene ID /WP=CE20061 /TR=Q9TZ22 /GB=AAC68929.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 35      4grp_total > 47      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 79.2
(3)
P 		25.3
(-1.3)
A 		22.2
(-1.6)
A 		4.4
(-3)
A 		75
B 56.4
(3)
P 		44.5
(0.9)
A 		22.6
(-3)
A 		28.6
(-1.9)
A 		34
C 66
(3)
P 		24.2
(-3)
A 		26.4
(-2.7)
A 		58.5
(1.9)
A 		42
avr 67.2
(3) 		31.3
(-2) 		23.7
(-3) 		30.5
(-2.1) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 67.8
(3)
A 		48.2
(0.5)
P 		50.4
(0.8)
A 		24.3
(-2.6)
A 		25.9
(-2.4)
A 		20.9
(-3)
A 		47
B 32.7
(-0.1)
A 		38.6
(1)
A 		50
(3)
A 		35.4
(0.4)
A 		20.8
(-2.2)
A 		16.5
(-3)
A 		34
C 18
(-1.5)
A 		58.3
(3)
A 		22.8
(-1)
A 		10.3
(-2.4)
A 		4.5
(-3)
A 		26.1
(-0.6)
A 		54
avr 39.5
(1.3) 		48.4
(3) 		41.1
(1.6) 		23.3
(-1.8) 		17.1
(-3) 		21.2
(-2.2) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 28.8
(3)
P 		5.5
(-2)
A 		5.8
(-1.9)
A 		0.6
(-3)
A 		28
B 18
(3)
P 		11
(-0.3)
A 		5.2
(-3)
A 		6.1
(-2.6)
A 		13
C 21.8
(3)
P 		7
(-3)
A 		9.2
(-2.1)
A 		9.9
(-1.8)
A 		15
avr 22.9
(3) 		7.8
(-2.2) 		6.7
(-2.6) 		5.5
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 12.5
(0.7)
A 		17.1
(3)
P 		11
(-0.1)
A 		6.2
(-2.5)
A 		7.8
(-1.7)
A 		5.1
(-3)
A 		12
B 11.4
(1.7)
A 		12.8
(3)
A 		12.1
(2.4)
A 		9.4
(-0)
A 		8.4
(-0.9)
A 		6.1
(-3)
A 		7
C 6.7
(-1.1)
A 		17.8
(3)
A 		5.8
(-1.4)
A 		2.1
(-2.8)
A 		1.5
(-3)
A 		8.7
(-0.3)
A 		16
avr 10.2
(-0.4) 		15.9
(3) 		9.6
(-0.8) 		5.9
(-3) 		5.9
(-3) 		6.6
(-2.6) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179866_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8207
Sequence Type Exemplar sequence
Representative Public ID T12B5.3 NCBI
Target Description T12B5.3 /REP_DB=WormBase Gene ID /WP=CE20061 /TR=Q9TZ22 /GB=AAC68929.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064903(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064903 NCBI Caenorhabditis elegans T12B5.3 (T12B5.3) mRNA, complete cds. 11/11 None
SNAP00000007703 ENSEMBL cdna:SNAP chromosome:CEL140:III:952531:955240:1 9/11 None
T12B5.3 ENSEMBL cdna:known chromosome:CEL140:III:952531:953790:1 gene:T12B5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:952530-953790(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.3
Entrez Gene 188442 Entrez gene
SwissProt Q9TZ22 EMBL-EBI
WormBase CE20061 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68929 Hypothetical protein T12B5.3 [Caenorhabditis elegans] ref|NP_497304.1| T12B5.3 [Caenorhabditis elegans] 1.0E-173
blast AAC68929 Hypothetical protein T12B5.3 [Caenorhabditis elegans] ref|NP_497304.1| T12B5.3 [Caenorhabditis elegans] 1.0E-135
blast AAC68930 Hypothetical protein T12B5.4 [Caenorhabditis elegans] ref|NP_497303.1| T12B5.4 [Caenorhabditis elegans] 5.0E-57
blast AAC04395 Hypothetical protein C17B7.11 [Caenorhabditis elegans] ref|NP_503929.1| C17B7.11 [Caenorhabditis elegans] 2.0E-53
blast CAB03494 Hypothetical protein ZC47.6 [Caenorhabditis elegans] ref|NP_497363.1| ZC47.6 [Caenorhabditis elegans] 7.0E-40
blast CAB03489 Hypothetical protein ZC47.4 [Caenorhabditis elegans] ref|NP_497367.1| ZC47.4 [Caenorhabditis elegans] 2.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.3E-57
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.0E-12
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.7E-40
Sequence
Target Sequence 
>C. ELEGANS:179866_AT
ggcaaaggactgtattcacgttaaggaaatacatttcaacaggcttttgttcgatgatat
tctctcaattcttccattcttcaatgctaaagtattggaaaatatcaagttatcggaaac
tgataatctgaaaaaatttgaacgactcgcttgcttggagcagtggaaaaacgcgaaaac
atttgaaactccttgggtcctggttcataattgtcaaattccaattgaacaattatttca
cctggagaggttcaaaatcgtcatagatgactttttcaaacaaaatagaattgatatcag
ggatgatttactgcaaaggagtacattccggaaatgcgaaattttgtttgaaaaatcgaa
ttcgacgccatttgaaattgctaaattgttcaagcctgacgtttctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCAAAGGACTGTATTCACGTTAAG 464 533 429 Antisense
GAAATACATTTCAACAGGCTTTTGT 435 363 454 Antisense
GGCTTTTGTTCGATGATATTCTCTC 658 537 470 Antisense
TGATATTCTCTCAATTCTTCCATTC 284 565 483 Antisense
TTGAACGACTCGCTTGCTTGGAGCA 351 703 566 Antisense
CATTTGAAACTCCTTGGGTCCTGGT 581 213 608 Antisense
GGGTCCTGGTTCATAATTGTCAAAT 305 287 623 Antisense
GAACAATTATTTCACCTGGAGAGGT 432 351 655 Antisense
GGAGAGGTTCAAAATCGTCATAGAT 75 519 672 Antisense
GAATTCGACGCCATTTGAAATTGCT 111 325 786 Antisense
AATTGTTCAAGCCTGACGTTTCTAA 430 177 812 Antisense
Sequence Source Affymetrix Proprietary Database