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[179865_at]

Description F54E7.5 /REP_DB=WormBase Gene ID /WP=CE01316 /TR=Q20783 /GB=AAK20074.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 35      6grp_cgc > 25      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 44.4
(-0.5)
A 		86.5
(3)
A 		13.4
(-3)
A 		50.2
(0)
A 		73
B 18.6
(-3)
A 		83.6
(3)
A 		33.3
(-1.6)
A 		53.4
(0.2)
A 		65
C 26.4
(-1.9)
A 		72.4
(3)
A 		16.2
(-3)
A 		46.4
(0.2)
A 		56
avr 29.8
(-2.1) 		80.8
(3) 		21
(-3) 		50
(-0.1) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 116.2
(3)
A 		107
(2.4)
A 		45.7
(-1.4)
A 		58.8
(-0.6)
A 		20
(-3)
A 		59.4
(-0.5)
A 		96
B 100.5
(3)
A 		91.6
(2.3)
A 		54.4
(-0.6)
A 		41.3
(-1.6)
A 		23.9
(-3)
A 		83
(1.6)
P 		77
C 83.4
(2)
A 		99.6
(3)
A 		70.2
(1.2)
A 		82
(1.9)
A 		3.9
(-3)
A 		65.2
(0.8)
P 		96
avr 100
(3) 		99.4
(3) 		56.8
(-0.1) 		60.7
(0.2) 		15.9
(-3) 		69.2
(0.8) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 16.2
(2)
A 		18.8
(3)
A 		3.5
(-3)
A 		7.3
(-1.5)
A 		15
B 5.9
(-3)
A 		20.6
(3)
A 		7.7
(-2.3)
A 		11.4
(-0.8)
A 		15
C 8.8
(-1.7)
A 		20.8
(3)
A 		5.6
(-3)
A 		7.8
(-2.1)
A 		15
avr 10.3
(-1.1) 		20.1
(3) 		5.6
(-3) 		8.8
(-1.7) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 21.4
(-0.1)
A 		38.1
(3)
A 		10
(-2.3)
A 		14.9
(-1.3)
A 		6
(-3)
A 		14.5
(-1.4)
A 		32
B 35
(3)
A 		30.5
(1.9)
A 		13.2
(-2.2)
A 		10.9
(-2.7)
A 		9.7
(-3)
A 		30.9
(2)
P 		25
C 30.9
(3)
A 		30.4
(2.9)
A 		17.9
(0.4)
A 		16.5
(0.1)
A 		1.3
(-3)
A 		21.8
(1.2)
P 		30
avr 29.1
(2.1) 		33
(3) 		13.7
(-1.2) 		14.1
(-1.2) 		5.7
(-3) 		22.4
(0.7) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179865_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7029
Sequence Type Exemplar sequence
Representative Public ID F54E7.5 NCBI
Target Description F54E7.5 /REP_DB=WormBase Gene ID /WP=CE01316 /TR=Q20783 /GB=AAK20074.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065814(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065814 NCBI Caenorhabditis elegans F54E7.5 (F54E7.5) mRNA, complete cds. 11/11 None
SNAP00000020780 ENSEMBL cdna:SNAP chromosome:CEL140:III:5655172:5656944:-1 11/11 None
GENEFINDER00000020791 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5655172:5661035:-1 11/11 None
F54E7.5 ENSEMBL cdna:known chromosome:CEL140:III:5655172:5656944:-1 gene:F54E7.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5655170-5656943(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sdz-21
Ensembl F54E7.5
Entrez Gene 186248 Entrez gene
SwissProt Q20783 EMBL-EBI
WormBase CE01316 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK20074 Hypothetical protein F54E7.5 [Caenorhabditis elegans] ref|NP_498215.1| F54E7.5 [Caenorhabditis elegans] 1.0E-155
blast AAK20075 Hypothetical protein F54E7.6 [Caenorhabditis elegans] ref|NP_498216.1| F54E7.6 [Caenorhabditis elegans] 1.0E-150
blast CAE64309 Hypothetical protein CBG08986 [Caenorhabditis briggsae] 1.0E-133
blast CAE64308 Hypothetical protein CBG08985 [Caenorhabditis briggsae] 9.0E-85
blast CAE64289 Hypothetical protein CBG08959 [Caenorhabditis briggsae] 4.0E-84
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498215.1 1 188-210
F54E7.5 1 188-210
SNAP00000020780 1 188-210
GENEFINDER00000020791 2 290-312,491-513
Sequence
Target Sequence 
>C. ELEGANS:179865_AT
ttgaatccattcggtcttgtcgcgaaatgtgaccagggatttcatccaaaagtgatcttc
aatcaatcatctactgatgttatgaatcgtctttccacacgtgtcatttttatcacttcc
caatgtttcactgcaaaggttttggttaaagtggatacagaatgcccatactgcccgcca
ccaactactattgctcctgatacattcaccgccaacaaatcattcatcttttcgacacaa
cacctcctcatcgcctgcctcattcttctctgcctaaccactttctgcatcatcgtcttc
ttcataactctccgccgactccgaaaacttcggatctctgatccatctctgaaatcagga
atgtccccaattcacaccaacgccacattcatttcgatttccgatgagaagccaattgta
tccaaatacatcgatttcttctctctgatgcctccgatgatcgaatatgatgaatctcat
ctcactgacagtttcactgttgctccaatgccacgtctaggatcccttgtctatgacttt
ttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAATCCATTCGGTCTTGTCGCGA 352 703 331 Antisense
TATGAATCGTCTTTCCACACGTGTC 48 657 411 Antisense
TTATCACTTCCCAATGTTTCACTGC 691 679 440 Antisense
TTCACCGCCAACAAATCATTCATCT 462 687 535 Antisense
GGATCTCTGATCCATCTCTGAAATC 56 513 662 Antisense
ACACCAACGCCACATTCATTTCGAT 325 109 704 Antisense
AATACATCGATTTCTTCTCTCTGAT 691 171 755 Antisense
TCTCTGATGCCTCCGATGATCGAAT 64 613 772 Antisense
GAATCTCATCTCACTGACAGTTTCA 163 329 802 Antisense
TTGCTCCAATGCCACGTCTAGGATC 515 699 830 Antisense
GGATCCCTTGTCTATGACTTTTTCC 331 513 850 Antisense
Sequence Source Affymetrix Proprietary Database