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[179857_s_at]

Description F22E5.1 /REP_DB=WormBase Gene ID /WP=CE09553 /TR=O16719 /GB=AAB66167.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 41      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 65
(-3)
A 		89.6
(2.8)
A 		87.9
(2.4)
A 		90.6
(3)
P 		26
B 43.6
(-3)
A 		129.1
(3)
A 		108.4
(1.5)
A 		58.2
(-2)
A 		86
C 60.6
(-3)
A 		120.4
(3)
M 		86.9
(-0.4)
M 		93.7
(0.3)
P 		60
avr 56.4
(-3) 		113
(3) 		94.4
(1) 		80.8
(-0.4) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 25.3
(-3)
A 		39.6
(-1.9)
A 		50.7
(-1)
A 		101.9
(3)
A 		93.4
(2.3)
A 		77
(1)
A 		77
B 60.8
(-2.7)
A 		59.2
(-3)
A 		65.6
(-1.8)
A 		67.4
(-1.4)
A 		80.6
(1.2)
A 		90
(3)
P 		31
C 69.7
(-1.2)
A 		47.8
(-3)
A 		50
(-2.8)
A 		111
(2.2)
A 		120.9
(3)
P 		107.9
(1.9)
A 		73
avr 51.9
(-2.6) 		48.9
(-3) 		55.4
(-2.2) 		93.4
(2.4) 		98.3
(3) 		91.6
(2.2) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 23.7
(3)
A 		19.5
(0.6)
A 		23
(2.6)
A 		13.3
(-3)
P 		10
B 13.9
(-2.5)
A 		31.9
(3)
A 		25.1
(0.9)
A 		12.4
(-3)
A 		20
C 20.1
(-1.6)
A 		34.6
(3)
M 		30.3
(1.6)
M 		15.8
(-3)
P 		19
avr 19.2
(-0.8) 		28.7
(3) 		26.1
(2) 		13.8
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 4.7
(-3)
A 		14.1
(-0.6)
A 		11
(-1.4)
A 		25.9
(2.4)
A 		28.2
(3)
A 		18.7
(0.6)
A 		24
B 21.2
(-1.2)
A 		19.7
(-1.7)
A 		15.9
(-3)
A 		17.9
(-2.3)
A 		32.7
(2.7)
A 		33.6
(3)
P 		18
C 25.8
(-0)
A 		14.6
(-2.6)
A 		12.7
(-3)
A 		22.3
(-0.8)
A 		39.1
(3)
P 		36.1
(2.3)
A 		26
avr 17.2
(-1.8) 		16.1
(-2.1) 		13.2
(-3) 		22
(-0.4) 		33.3
(3) 		29.5
(1.9) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179857_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5192
Sequence Type Exemplar sequence
Representative Public ID F22E5.1 NCBI
Target Description F22E5.1 /REP_DB=WormBase Gene ID /WP=CE09553 /TR=O16719 /GB=AAB66167.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061928(11), NM_062065(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061928 NCBI Caenorhabditis elegans F22E5.1 (F22E5.1) mRNA, complete cds. 11/11 A 
NM_062065 NCBI Caenorhabditis elegans Y27F2A.6 (Y27F2A.6) mRNA, complete cds. 11/11 A 
SNAP00000006768 ENSEMBL cdna:SNAP chromosome:CEL140:II:3181544:3182683:-1 11/11 None
GENEFINDER00000006778 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3180535:3182683:-1 11/11 None
SNAP00000023619 ENSEMBL cdna:SNAP chromosome:CEL140:II:2675789:2677538:1 11/11 None
GENEFINDER00000023630 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2675789:2678151:1 10/11 None
F22E5.1 ENSEMBL cdna:known chromosome:CEL140:II:2675789:2677538:1 gene:F22E5.1 11/11 A 
Y27F2A.6 ENSEMBL cdna:known chromosome:CEL140:II:3180535:3182671:-1 gene:Y27F2A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2675787-2677537(+) 100.0 100.0
chrII:3181543-3182682(-) 80.41 80.41
Public Domain and Genome References
Gene Title

Ensembl F22E5.1
Y27F2A.6
Entrez Gene 184831 Entrez gene
189560 Entrez gene
SwissProt O16719 EMBL-EBI
Q9N548 EMBL-EBI
WormBase CE09553 Wormbase
CE21493 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494329 F22E5.1 [Caenorhabditis elegans] gb|AAB66167.1| Hypothetical protein F22E5.1 [Caenorhabditis elegans] 0.0
blast NP_494466 Y27F2A.6 [Caenorhabditis elegans] gb|AAF60473.1| Hypothetical protein Y27F2A.6 [Caenorhabditis elegans] 0.0
blast NP_494329 F22E5.1 [Caenorhabditis elegans] gb|AAB66167.1| Hypothetical protein F22E5.1 [Caenorhabditis elegans] 1.0E-169
blast NP_494466 Y27F2A.6 [Caenorhabditis elegans] gb|AAF60473.1| Hypothetical protein Y27F2A.6 [Caenorhabditis elegans] 1.0E-163
blast NP_494329 F22E5.1 [Caenorhabditis elegans] gb|AAB66167.1| Hypothetical protein F22E5.1 [Caenorhabditis elegans] 1.0E-158
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494329.1 1 7-26
NP_494466.1 1 5-22
F22E5.1 1 7-26
Y27F2A.6 1 5-22
SNAP00000006768 1 7-26
GENEFINDER00000006778 1 7-26
SNAP00000023619 1 7-26
GENEFINDER00000023630 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:179857_S_AT
cggctacccggattatacgaatcattggatggagtatcggtttaagccactgctcctagc
ggagctcctgaaagagcatccgaatatttggtggatggatgcacatttgtcagtgaagaa
gcctaatatggtggagctgatgatgaaggaggttgcagaaaaaactaagaaatctgagac
aaaagtgacagtctccatatacttcttcatttattccagccacagcaactttgccacatt
aaacacagctctgctggactacttcccaacaaattcgataggccttctgaaggacccgaa
cctcggatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGCTACCCGGATTATACGAATCAT 595 239 558 Antisense
GATGCACATTTGTCAGTGAAGAAGC 438 413 655 Antisense
GCCTAATATGGTGGAGCTGATGATG 646 281 678 Antisense
AACTTTGCCACATTAAACACAGCTC 320 139 784 Antisense
TGCCACATTAAACACAGCTCTGCTG 244 587 789 Antisense
AACACAGCTCTGCTGGACTACTTCC 597 133 799 Antisense
TCTGCTGGACTACTTCCCAACAAAT 384 609 807 Antisense
GGACTACTTCCCAACAAATTCGATA 307 525 813 Antisense
TTCCCAACAAATTCGATAGGCCTTC 639 695 820 Antisense
GATAGGCCTTCTGAAGGACCCGAAC 290 425 834 Antisense
TCTGAAGGACCCGAACCTCGGATCC 579 599 843 Antisense
Sequence Source Affymetrix Proprietary Database