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[179852_at]

Description F35E12.2 /REP_DB=WormBase Gene ID /WP=CE09917 /TR=O02355 /GB=CAB04270.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 130.4
(-1.5)
A 		116.7
(-3)
A 		149.7
(0.5)
A 		172.9
(3)
P 		56
B 116.5
(-1.3)
P 		109.1
(-3)
A 		112.6
(-2.2)
A 		135.1
(3)
P 		26
C 129.9
(-1.6)
M 		104.4
(-2.5)
A 		89.8
(-3)
A 		262.9
(3)
P 		173
avr 125.6
(-1.8) 		110.1
(-3) 		117.4
(-2.5) 		190.3
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 59.5
(-3)
A 		124.4
(3)
P 		111.2
(1.8)
P 		116.3
(2.3)
P 		109.7
(1.6)
A 		69
(-2.1)
A 		65
B 71.3
(-0.7)
M 		112.8
(3)
P 		107.7
(2.5)
A 		45.2
(-3)
A 		50.3
(-2.5)
A 		75
(-0.4)
M 		68
C 82.6
(0.9)
A 		111.4
(2.6)
P 		119.3
(3)
M 		12.4
(-3)
A 		97.8
(1.8)
P 		67.9
(0.1)
A 		107
avr 71.1
(-1.6) 		116.2
(3) 		112.7
(2.6) 		58
(-3) 		85.9
(-0.1) 		70.6
(-1.7) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 47.5
(3)
A 		25.3
(-3)
A 		39.1
(0.7)
A 		25.3
(-3)
P 		22
B 37.1
(3)
P 		26.9
(-2.6)
A 		26.1
(-3)
A 		28.8
(-1.5)
P 		11
C 43
(2.5)
M 		30
(-3)
A 		31.3
(-2.5)
A 		44.3
(3)
P 		14
avr 42.5
(3) 		27.4
(-3) 		32.2
(-1.1) 		32.8
(-0.9) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 11
(-3)
A 		44.3
(3)
P 		24.2
(-0.6)
P 		29.5
(0.3)
P 		33.1
(1)
A 		16.8
(-2)
A 		33
B 24.8
(0)
M 		37.6
(3)
P 		26.1
(0.3)
A 		12
(-3)
A 		20.4
(-1)
A 		28
(0.8)
M 		26
C 30.6
(2.4)
A 		34
(3)
P 		30.4
(2.3)
M 		2.5
(-3)
A 		31.6
(2.5)
P 		22.7
(0.8)
A 		32
avr 22.1
(-1.1) 		38.6
(3) 		26.9
(0.1) 		14.7
(-3) 		28.4
(0.4) 		22.5
(-1) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179852_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12629
Sequence Type Exemplar sequence
Representative Public ID F35E12.2 NCBI
Target Description F35E12.2 /REP_DB=WormBase Gene ID /WP=CE09917 /TR=O02355 /GB=CAB04270.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073996(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073996 NCBI Caenorhabditis elegans F35E12.2 (F35E12.2) mRNA, complete cds. 11/11 None
SNAP00000032227 ENSEMBL cdna:SNAP chromosome:CEL140:V:13725948:13727471:-1 11/11 None
GENEFINDER00000032239 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13724936:13726962:-1 11/11 None
F35E12.2 ENSEMBL cdna:known chromosome:CEL140:V:13725948:13727471:-1 gene:F35E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13725947-13727471(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E12.2
Entrez Gene 185304 Entrez gene
SwissProt O02355 EMBL-EBI
WormBase CE09917 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04270 Hypothetical protein F35E12.2 [Caenorhabditis elegans] ref|NP_506397.1| F35E12.2 [Caenorhabditis elegans] 0.0
blast CAE66255 Hypothetical protein CBG11499 [Caenorhabditis briggsae] 1.0E-154
blast CAE66255 Hypothetical protein CBG11499 [Caenorhabditis briggsae] 1.0E-147
blast CAB04270 Hypothetical protein F35E12.2 [Caenorhabditis elegans] ref|NP_506397.1| F35E12.2 [Caenorhabditis elegans] 1.0E-133
blast CAE66255 Hypothetical protein CBG11499 [Caenorhabditis briggsae] 1.0E-103
blast CAD54131 Hypothetical protein F10A3.4 [Caenorhabditis elegans] ref|NP_872187.1| F10A3.4 [Caenorhabditis elegans] 6.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.4E-25
Sequence
Target Sequence 
>C. ELEGANS:179852_AT
ggttgccctcaacattgctgttaaaaacacgcaggattacgacgctcttctcgattttat
atttgtctatgactgtggagatgttacaaattgcagaatggtcgctcgactttcgaattt
cttaaaaagacaaaaatactcaacatcaaataatatcactattgtgaatttttacgacgt
taaatctccatcttatttgattggaaatgacttttcaaaaattgtggtgttttcaaagta
taaagctattttgacaagccgaaatcaaagaacaactggagaattgtttgatgcaagtga
tcagggaacagcttatacttggatatgctcggattgtcaacatttttattggacagtact
caaatttcataaatcaaaaacaaatatctcaaaaacttacattgaactccgtccgttgtc
acctacttacaacacttcaaccctgcttaactatacgatgtcttctgcactggactctta
ctttcctcaattgattcccgcgcgaatgttcacaatgataaatcattacagtggcatcgc
agttacattggaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTGCCCTCAACATTGCTGTTAAA 248 507 513 Antisense
AAACACGCAGGATTACGACGCTCTT 475 129 537 Antisense
GACGCTCTTCTCGATTTTATATTTG 418 381 553 Antisense
ATGGTCGCTCGACTTTCGAATTTCT 495 51 610 Antisense
AACTTACATTGAACTCCGTCCGTTG 43 135 906 Antisense
GTCCGTTGTCACCTACTTACAACAC 121 475 923 Antisense
ACTTCAACCCTGCTTAACTATACGA 111 103 946 Antisense
TACGATGTCTTCTGCACTGGACTCT 246 647 966 Antisense
GACTCTTACTTTCCTCAATTGATTC 401 373 985 Antisense
TTGATTCCCGCGCGAATGTTCACAA 60 705 1003 Antisense
AGTGGCATCGCAGTTACATTGGACA 270 59 1042 Antisense
Sequence Source Affymetrix Proprietary Database