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[179831_at]

Description F26E4.10 /REP_DB=WormBase Gene ID /WP=CE28007 /TR=O01327 /GB=CAB03006.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 57      4grp_high_8_8 > 11      6grp_total > 68      4grp_total > 47      6grp_cgc > 28      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 67.6
(-2.3)
P 		68.9
(-2)
P 		63
(-3)
P 		99.8
(3)
P 		37
B 95.1
(3)
P 		52.1
(-1.2)
M 		71.7
(0.7)
P 		33.2
(-3)
A 		62
C 86.7
(3)
P 		81
(-3)
P 		81.2
(-2.8)
P 		82.9
(-1)
P 		6
avr 83.1
(3) 		67.3
(-3) 		72
(-1.2) 		72
(-1.2) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 76.8
(-0.5)
A 		97.3
(1.5)
P 		50.8
(-3)
P 		102.1
(2)
P 		77
(-0.5)
P 		112.6
(3)
P 		62
B 83.1
(-1)
P 		71.5
(-3)
P 		81.8
(-1.2)
P 		73
(-2.7)
P 		106
(3)
P 		102.2
(2.3)
P 		35
C 102.6
(2.1)
P 		85.2
(0.1)
P 		97.6
(1.5)
P 		71
(-1.6)
P 		58.8
(-3)
P 		110.5
(3)
P 		52
avr 87.5
(-1) 		84.7
(-1.5) 		76.7
(-3) 		82
(-2) 		80.6
(-2.3) 		108.4
(3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 24.6
(3)
P 		15
(-2.8)
P 		16.5
(-1.9)
P 		14.6
(-3)
P 		10
B 30.3
(3)
P 		12.9
(-1.5)
M 		16.6
(-0.5)
P 		7.1
(-3)
A 		23
C 28.7
(3)
P 		23.3
(0.8)
P 		28.3
(2.8)
P 		14
(-3)
P 		15
avr 27.9
(3) 		17.1
(-1.1) 		20.5
(0.2) 		11.9
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 14.2
(-2.2)
A 		34.6
(3)
P 		11.1
(-3)
P 		25.9
(0.8)
P 		23.3
(0.1)
P 		27.4
(1.2)
P 		24
B 28.9
(-0.6)
P 		23.8
(-1.9)
P 		19.9
(-2.8)
P 		19.3
(-3)
P 		43.1
(3)
P 		38.1
(1.7)
P 		24
C 38
(3)
P 		26
(-0)
P 		24.8
(-0.3)
P 		14.3
(-3)
P 		19
(-1.8)
P 		37
(2.7)
P 		24
avr 27
(0.2) 		28.1
(0.7) 		18.6
(-3) 		19.8
(-2.5) 		28.5
(0.8) 		34.2
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179831_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.393
Sequence Type Exemplar sequence
Representative Public ID F26E4.10 NCBI
Target Description F26E4.10 /REP_DB=WormBase Gene ID /WP=CE28007 /TR=O01327 /GB=CAB03006.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060198(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060198 NCBI Caenorhabditis elegans Ortholog of Drosophila DRoSHa gene family member (drsh-1) (drsh-1) mRNA, complete cds. 11/11 A 
SNAP00000026229 ENSEMBL cdna:SNAP chromosome:CEL140:I:9789592:9793843:-1 11/11 None
GENEFINDER00000026237 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9789592:9793843:-1 11/11 None
F26E4.10 ENSEMBL cdna:known chromosome:CEL140:I:9789447:9793843:-1 gene:F26E4.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9791240-9793774(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol drsh-1
Ensembl F26E4.10
Entrez Gene 172830 Entrez gene
SwissProt O01326 EMBL-EBI
WormBase CE29039 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1593402_S_AT similar to Ribonuclease III (RNase III) (Drosha) (p241) cfa
CANINE_2:CFA.1260.1.A1_S_AT similar to Ribonuclease III (RNase III) (Drosha) (p241) cfa
CANINE_2:CFAAFFX.28945.1.S1_S_AT similar to Ribonuclease III (RNase III) (Drosha) (p241) cfa
DROSOPHILA_2:1630216_AT dm
DROSGENOME1:154515_AT dm
CHICKEN:GGAAFFX.12824.1.S1_AT ribonuclease III, nuclear gga
HG-U133_PLUS_2:218269_AT ribonuclease III, nuclear hs
HG-U133A:218269_AT ribonuclease III, nuclear hs
HG-FOCUS:218269_AT ribonuclease III, nuclear hs
HG-U133A_2:218269_AT ribonuclease III, nuclear hs
HU35KSUBA:HUM_ALU_AT ribonuclease III, nuclear hs
HU35KSUBB:HUM_ALU_AT ribonuclease III, nuclear hs
HU35KSUBC:HUM_ALU_AT ribonuclease III, nuclear hs
HU35KSUBD:HUM_ALU_AT ribonuclease III, nuclear hs
HUGENEFL:HUM_ALU_AT ribonuclease III, nuclear hs
HC-G110:HUM_ALU_AT ribonuclease III, nuclear hs
HU35KSUBC:RC_AA459956_AT ribonuclease III, nuclear hs
U133_X3P:G7106396_3P_AT ribonuclease III, nuclear hs
HG-U95C:62181_AT ribonuclease III, nuclear hs
HG-U95E:88331_AT ribonuclease III, nuclear hs
HU35KSUBC:RC_N64604_AT Ribonuclease III, nuclear hs
HG-U95C:53235_AT Ribonuclease III, nuclear hs
HG-U133_PLUS_2:241269_AT Ribonuclease III, nuclear hs
HG-U133B:241269_AT Ribonuclease III, nuclear hs
HG-U95B:57307_AT Ribonuclease III, nuclear hs
HG-U95D:70979_AT Ribonuclease III, nuclear hs
HU35KSUBD:RC_AA405978_AT Ribonuclease III, nuclear hs
U133_X3P:HS.97997.0.A1_3P_AT Ribonuclease III, nuclear hs
MOUSE430_2:1438440_AT ribonuclease III, nuclear mm
MOE430B:1438440_AT ribonuclease III, nuclear mm
MG-U74BV2:115382_AT ribonuclease III, nuclear mm
MG-U74BV2:107753_AT ribonuclease III, nuclear mm
MOE430B:1428656_AT ribonuclease III, nuclear mm
MOUSE430_2:1428656_AT ribonuclease III, nuclear mm
MOE430B:1438505_S_AT ribonuclease III, nuclear mm
MOUSE430_2:1438505_S_AT ribonuclease III, nuclear mm
MU19KSUBC:TC38375_AT ribonuclease III, nuclear mm
MU19KSUBC:TC40958_AT ribonuclease III, nuclear mm
MG-U74CV2:139239_AT Ribonuclease III (Rn3) mm
MU19KSUBB:TC31657_AT Ribonuclease III (Rn3) mm
MU19KSUBC:TC40943_AT Ribonuclease III (Rn3) mm
RAE230A:1376273_AT similar to Ribonuclease III (RNase III) (p241) (predicted) rn
RAT230_2:1376273_AT similar to Ribonuclease III (RNase III) (p241) (predicted) rn
RAE230A:1375736_AT Similar to Ribonuclease III (RNase III) (p241) (predicted) rn
RAT230_2:1375736_AT Similar to Ribonuclease III (RNase III) (p241) (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6396 RNA processing inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
3725 double-stranded RNA binding inferred from electronic annotation QuickGO AmiGO
4525 ribonuclease III activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03006 Hypothetical protein F26E4.10 [Caenorhabditis elegans] ref|NP_492599.1| Ortholog of Drosophila DRoSHa gene family member (drsh-1) [Caenorhabditis elegans] sp|O01326|RNC_CAEEL Ribonuclease III (RNase III) 0.0
blast AAD45518 ribonuclease [Caenorhabditis elegans] 0.0
blast CAE60227 Hypothetical protein CBG03798 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000999 EMBL-EBI Ribonuclease III 3.2E-46
Pfam IPR000999 EMBL-EBI Ribonuclease III 8.5E-44
Pfam IPR001159 EMBL-EBI Double-stranded RNA binding (DsRBD) domain 1.1E-14
Pfam IPR000999 EMBL-EBI Ribonuclease III 3.2E-46
Pfam IPR000999 EMBL-EBI Ribonuclease III 8.5E-44
Pfam IPR001159 EMBL-EBI Double-stranded RNA binding (DsRBD) domain 1.1E-14
Sequence
Target Sequence 
>C. ELEGANS:179831_AT
caaaattcaaaccttctgctatcagagccgattggtttgagagagatgaagaaaagaaag
aagtatatgttgtacataatgcgatccgtgcacagacatatacagcaatttcgcttccaa
gaatcgcatttctcgagaaaacattaaataagatgatacaagagaaacaaagcagtggtg
tttataataaagattttgagaaaacgaagaacgagctggaacatctgaaaagagagaatc
gatcagctcgaaatctgaagcttcgggagccagtagctggattcattgaaacaggattga
agcctgatgtggcagcacatgttgtcatgacaatacttgcatgccatcatattcgataca
atttttcattggatgtatttgaagaagtcatcgaatataaattcaacgatagaagagtca
tcgaattagcgttaatgcattcgtcgtttaaaagtcattatggtactccaattgatcacg
tca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAATTCAAACCTTCTGCTATCAG 398 189 1463 Antisense
TCTGCTATCAGAGCCGATTGGTTTG 216 607 1477 Antisense
GTTGTACATAATGCGATCCGTGCAC 469 437 1531 Antisense
GCGATCCGTGCACAGACATATACAG 432 289 1543 Antisense
GCAATTTCGCTTCCAAGAATCGCAT 316 323 1567 Antisense
GAAGCTTCGGGAGCCAGTAGCTGGA 375 341 1719 Antisense
GGATTGAAGCCTGATGTGGCAGCAC 353 509 1756 Antisense
GTGGCAGCACATGTTGTCATGACAA 459 491 1771 Antisense
ATACTTGCATGCCATCATATTCGAT 174 21 1795 Antisense
TTAGCGTTAATGCATTCGTCGTTTA 417 651 1888 Antisense
TATGGTACTCCAATTGATCACGTCA 262 655 1921 Antisense
Sequence Source Affymetrix Proprietary Database