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[179827_at]

Description T03A1.3 /REP_DB=WormBase Gene ID /WP=CE18187 /TR=O61842 /GB=AAC17551.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 48      4grp_total > 12      6grp_cgc > 19      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 3.1
(-3)
A 		4.7
(-1.6)
A 		4
(-2.2)
A 		10
(3)
A 		7
B 4.7
(2.2)
A 		5.1
(3)
A 		2
(-3)
A 		2.4
(-2.2)
A 		3
C 19.5
(-0.3)
A 		4.2
(-2.7)
A 		2.4
(-3)
A 		40.9
(3)
A 		39
avr 9.1
(-0.5) 		4.7
(-2.2) 		2.8
(-3) 		17.8
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 6.6
(1.8)
A 		3.9
(-0.8)
A 		1.7
(-2.8)
A 		2.1
(-2.4)
A 		1.5
(-3)
A 		7.9
(3)
A 		6
B 2.3
(-1.9)
A 		3.6
(1.6)
A 		1.9
(-3)
A 		2.1
(-2.5)
A 		3.8
(2.2)
A 		4.1
(3)
A 		2
C 2.4
(-2.8)
A 		5.9
(3)
A 		3.4
(-1.2)
A 		2.3
(-3)
A 		4.4
(0.5)
A 		3.5
(-1)
A 		4
avr 3.8
(0.2) 		4.5
(1.6) 		2.3
(-2.8) 		2.2
(-3) 		3.2
(-1) 		5.2
(3) 		3
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 1.1
(-1.8)
A 		1
(-3)
A 		1
(-3)
A 		1.5
(3)
A 		1
B 1.5
(3)
A 		1.3
(1.8)
A 		0.5
(-3)
A 		0.5
(-3)
A 		1
C 6.5
(2.6)
A 		1.2
(-2.7)
A 		0.9
(-3)
A 		6.9
(3)
A 		6
avr 3
(3) 		1.2
(-1.9) 		0.8
(-3) 		3
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 1.2
(0.2)
A 		1.4
(1)
A 		0.4
(-3)
A 		0.5
(-2.6)
A 		0.5
(-2.6)
A 		1.9
(3)
A 		2
B 0.8
(-1.4)
A 		1.2
(0.8)
A 		0.5
(-3)
A 		0.6
(-2.5)
A 		1.6
(3)
A 		1.5
(2.5)
A 		1
C 0.9
(-1.2)
A 		1.8
(3)
A 		0.9
(-1.2)
A 		0.5
(-3)
A 		1.4
(1.2)
A 		1.2
(0.2)
A 		1
avr 1
(0) 		1.5
(3) 		0.6
(-2.4) 		0.5
(-3) 		1.2
(1.2) 		1.5
(3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179827_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1721
Sequence Type Exemplar sequence
Representative Public ID T03A1.3 NCBI
Target Description T03A1.3 /REP_DB=WormBase Gene ID /WP=CE18187 /TR=O61842 /GB=AAC17551.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058952(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058952 NCBI Caenorhabditis elegans abnormal nuclear ANChorage family member (anc-1) (anc-1) mRNA, complete cds. 9/11 None
GENEFINDER00000023526 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4321663:4358873:1 9/11 None
SNAP00000023505 ENSEMBL cdna:SNAP chromosome:CEL140:I:4329659:4330673:1 11/11 None
ZK973.6 ENSEMBL cdna:known chromosome:CEL140:I:4321096:4359081:1 gene:ZK973.6 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4329658-4330673(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol anc-1
Ensembl ZK973.6
Entrez Gene 172034 Entrez gene
SwissProt Q9N4M4 EMBL-EBI
WormBase CE33588 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1585356_AT similar to nesprin 1 isoform beta cfa
CANINE:1591393_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.11044.1.A1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.14004.1.A1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.14004.1.A1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.20729.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1680.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1700.1.S1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1717.1.S1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1717.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1722.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1723.1.S1_AT similar to nesprin 1 isoform beta cfa
CHICKEN:GGA.15842.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGA.15842.1.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGA.18878.1.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGA.9526.1.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.24072.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8215.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8215.2.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8600.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8667.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
HG-U133_PLUS_2:215350_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A_2:215350_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A:215350_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:209447_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A:209447_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A_2:209447_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBA:RC_AA452410_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBB:RC_AA227537_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBB:RC_AA218915_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBC:RC_N23629_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:G2895592_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.272567.0.S1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.241420.0.S1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.192102.0.A1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.161707.0.A1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:215350_3P_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:232027_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133B:232027_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:244070_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133B:244070_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:244144_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133B:244144_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95AV2:38113_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95B:42634_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95C:63705_F_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95C:63906_F_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:72970_G_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:85140_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:88102_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95C:63924_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95E:67418_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:68235_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:72968_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:82696_AT Spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBC:RC_N71774_AT Spectrin repeat containing, nuclear envelope 1 hs
RG-U34A:X95466_AT CPG2 protein rn
RG-U34C:RC_AI070400_AT CPG2 protein rn
RG-U34C:RC_AI072953_S_AT CPG2 protein rn
RAE230A:1370264_AT CPG2 protein rn
RAT230_2:1370264_AT CPG2 protein rn
RAE230A:1398263_AT CPG2 protein rn
RAT230_2:1398263_AT CPG2 protein rn
RAE230B:1378886_X_AT CPG2 protein rn
RAT230_2:1378886_X_AT CPG2 protein rn
RAE230B:1398499_AT CPG2 protein rn
RAT230_2:1398499_AT CPG2 protein rn
RAE230B:1396587_AT CPG2 protein rn
RAT230_2:1396587_AT CPG2 protein rn
RAE230B:1398155_AT CPG2 protein rn
RAT230_2:1398155_AT CPG2 protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6915 apoptosis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
3779 actin binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF40010 Abnormal nuclear anchorage protein 1 [Caenorhabditis elegans] ref|NP_491353.2| abnormal nuclear ANChorage family member (anc-1) [Caenorhabditis elegans] sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog) 0.0
blast tpg|DAA04553.1 TPA: ANC-1 [Caenorhabditis elegans] 0.0
blast AAN35200 ANC-1 [Caenorhabditis elegans] 0.0
blast T33141 hypothetical protein T03A1.3 - Caenorhabditis elegans 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.3E-30
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.6E-28
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 3.3E-17
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.6E-28
Sequence
Target Sequence 
>C. ELEGANS:179827_AT
gttacgcttttgtctatgacgagtataatcaggtaccggtgaacctaaccgaaaacgtgt
tgagcgaagattacgttcgtgaaatcgtcaatcgaaagaaggcaattctgactcgccgcg
agaacaacgaggatgagatccgtgaatcgattcgcgatatggaagaatgcgatcggatta
ttgaaggatggcagagcaccgaaatcgaggaactgcgtgctgaatggaatatcaaattgt
cggaattcgaatcatggcatgagatgatgcagcaagttgaagtactttcccagacaattc
agaccagattagatgtgaccgtcattcaaacgatctggatactgaaggaacgttcctatg
atatcaagcatagtgaactgggaggaactcttcgagaatctctggaacaacttgcaacga
ctagtgaaacatctgtcaatcggcatctacaaaatttagagctcagcaatgagcaggact
gtccagatgccatcgaatttctagaggtgagtatagtcattttccgctcattacactctt
ttcattctcctcttatcttatatcctctcaactttttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTACGCTTTTGTCTATGACGAGTA 83 447 74 Antisense
AAAGAAGGCAATTCTGACTCGCCGC 572 121 168 Antisense
GATGAGATCCGTGAATCGATTCGCG 120 411 205 Antisense
GAAATCGAGGAACTGCGTGCTGAAT 210 363 274 Antisense
GTACTTTCCCAGACAATTCAGACCA 435 459 355 Antisense
GTGACCGTCATTCAAACGATCTGGA 184 483 388 Antisense
GAGAATCTCTGGAACAACTTGCAAC 414 391 467 Antisense
GAAACATCTGTCAATCGGCATCTAC 10 357 499 Antisense
CAGGACTGTCCAGATGCCATCGAAT 333 209 547 Antisense
GTATAGTCATTTTCCGCTCATTACA 66 453 584 Antisense
TCTTATATCCTCTCAACTTTTTTTC 172 605 629 Antisense
Sequence Source Affymetrix Proprietary Database