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[179826_s_at]

Description F23C8.9 /REP_DB=WormBase Gene ID /WP=CE20718 /TR=Q9TXI0 /GB=AAD03137.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 40      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 19
(-3)
A 		94.3
(1.7)
A 		114.8
(3)
A 		92.6
(1.6)
A 		96
B 62.3
(-3)
A 		120.4
(1.2)
A 		145.1
(3)
A 		65.3
(-2.8)
A 		83
C 66.1
(-3)
A 		123.9
(3)
A 		120.1
(2.6)
P 		73.1
(-2.3)
A 		58
avr 49.1
(-3) 		112.9
(1.9) 		126.7
(3) 		77
(-0.8) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 77.3
(-2.3)
A 		66.1
(-3)
A 		105.8
(-0.5)
A 		74
(-2.5)
A 		94.7
(-1.2)
A 		162.9
(3)
P 		97
B 80.4
(-2.8)
A 		79.3
(-2.9)
A 		99.1
(-0.4)
A 		100.2
(-0.3)
A 		78.7
(-3)
A 		126.6
(3)
P 		48
C 80.1
(-1.3)
A 		95.8
(1.7)
A 		81.8
(-1)
A 		71.1
(-3)
A 		89
(0.4)
A 		102.6
(3)
P 		32
avr 79.3
(-3) 		80.4
(-2.9) 		95.6
(-1.1) 		81.8
(-2.7) 		87.5
(-2) 		130.7
(3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 6.9
(-3)
A 		20.5
(0.5)
A 		30
(3)
A 		13.6
(-1.3)
A 		23
B 19.9
(-1.2)
A 		29.7
(1.8)
A 		33.6
(3)
A 		13.9
(-3)
A 		20
C 21.9
(-1.1)
A 		35.6
(1.7)
A 		41.9
(3)
P 		12.3
(-3)
A 		30
avr 16.2
(-2.2) 		28.6
(1.2) 		35.2
(3) 		13.3
(-3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 14.2
(-3)
A 		23.5
(-0.8)
A 		23
(-0.9)
A 		18.8
(-1.9)
A 		28.6
(0.4)
A 		39.7
(3)
P 		26
B 28
(-2)
A 		26.4
(-2.4)
A 		24.1
(-3)
A 		26.5
(-2.4)
A 		32
(-0.9)
A 		47.2
(3)
P 		23
C 29.7
(1.6)
A 		29.2
(1.5)
A 		20.8
(-1.1)
A 		14.3
(-3)
A 		28.8
(1.4)
A 		34.3
(3)
P 		20
avr 24
(-1.8) 		26.4
(-1.1) 		22.6
(-2.2) 		19.9
(-3) 		29.8
(-0.1) 		40.4
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179826_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.201
Sequence Type Exemplar sequence
Representative Public ID F23C8.9 NCBI
Target Description F23C8.9 /REP_DB=WormBase Gene ID /WP=CE20718 /TR=Q9TXI0 /GB=AAD03137.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058576(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058576 NCBI Caenorhabditis elegans F23C8.9 (F23C8.9) mRNA, complete cds. 11/11 None
SNAP00000018137 ENSEMBL cdna:SNAP chromosome:CEL140:I:2432711:2433610:-1 11/11 None
GENEFINDER00000018151 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2432711:2433610:-1 11/11 None
F23C8.9 ENSEMBL cdna:known chromosome:CEL140:I:2432600:2433610:-1 gene:F23C8.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2432710-2433610(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F23C8.9
Entrez Gene 171803 Entrez gene
SwissProt Q9TXI0 EMBL-EBI
WormBase CE20718 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD03137 Hypothetical protein F23C8.9 [Caenorhabditis elegans] ref|NP_490977.1| F23C8.9 [Caenorhabditis elegans] 3.0E-91
blast CAE60384 Hypothetical protein CBG03985 [Caenorhabditis briggsae] 1.0E-60
blast AAF53690 CG10336-PA, isoform A [Drosophila melanogaster] gb|AAM48472.1| SD08134p [Drosophila melanogaster] ref|NP_724124.1| CG10336-PA, isoform A [Drosophila melanogaster] 8.0E-15
Sequence
Target Sequence 
>C. ELEGANS:179826_S_AT
aacagcggcgagggaggcactagacgagtcgtggagccgaaattgttaaggactaaaaga
ctagcaaatccacgcctagcactgaatgagcgaattttgaccgggccgaaaggaatttca
gctcttcgggagactttaaaagatttcaagccaaatcccaaggatgatccgtacgcaaat
ctggagaaattgatgaaaaaatacgcatactggggacatttgatgtttccgaagatgaaa
accgaagatgttctgaatcgagtcgagacactgggaacacggcggcaggttaaagttttc
atgatcaagcaccggcttggagagactggcgaggattctgagcacgaggagcaggagaat
caaaagaatggaattattgatgatggagcgagtgataatgacgatgatttattcaaggat
ttaccagagaaagaagtcacgactgaaaaagctaaaaattctgaaaaatccgaccaaaaa
accgctgaaattgatgaaaatgtcgaggaagagtatcgaatgatggaggaagagcggctg
cgagaggaacaggaagccaaggaagctgctgacgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAGCGGCGAGGGAGGCACTAGAC 47 135 94 Antisense
GGCACTAGACGAGTCGTGGAGCCGA 530 535 109 Antisense
AATCCACGCCTAGCACTGAATGAGC 524 165 160 Antisense
GAATTTCAGCTCTTCGGGAGACTTT 626 325 206 Antisense
AAGGATGATCCGTACGCAAATCTGG 255 161 253 Antisense
TCGAGACACTGGGAACACGGCGGCA 669 601 356 Antisense
CACGGCGGCAGGTTAAAGTTTTCAT 247 1 371 Antisense
AAGTTTTCATGATCAAGCACCGGCT 632 155 386 Antisense
AGCACCGGCTTGGAGAGACTGGCGA 531 77 401 Antisense
GACTGGCGAGGATTCTGAGCACGAG 147 377 417 Antisense
GGAAGCCAAGGAAGCTGCTGACGAA 78 531 645 Antisense
Sequence Source Affymetrix Proprietary Database