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[179815_at]

Description T23F4.3 /REP_DB=WormBase Gene ID /WP=CE14088 /TR=O17263 /GB=AAB71032.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 82      4grp_high_8_8 > 11      6grp_highest_nonorm > 71      6grp_total > 73      4grp_total > 13      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 234.5
(0.1)
P 		110.2
(-2.4)
P 		80.4
(-3)
P 		382.8
(3)
P 		302
B 269.4
(1.2)
P 		122.9
(-1.6)
A 		52
(-3)
A 		365.2
(3)
P 		313
C 242.2
(0.2)
P 		109.2
(-2)
P 		53.1
(-3)
P 		406.5
(3)
P 		353
avr 248.7
(0.5) 		114.1
(-2) 		61.8
(-3) 		384.8
(3) 		323

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 415
1 2 3 4 5 6 range
A 113.9
(-3)
P 		156.7
(-2.4)
P 		370.6
(0.7)
P 		529.1
(3)
P 		417.2
(1.4)
P 		295.6
(-0.4)
P 		415
B 145.6
(-3)
P 		164.7
(-2.7)
P 		412.2
(1.5)
P 		500.3
(3)
P 		476.2
(2.6)
P 		252.2
(-1.2)
P 		355
C 163.3
(-3)
P 		168.7
(-2.9)
P 		483.8
(2.7)
P 		499.3
(3)
P 		494.7
(2.9)
P 		253.1
(-1.4)
P 		336
avr 140.9
(-3) 		163.4
(-2.6) 		422.2
(1.6) 		509.6
(3) 		462.7
(2.2) 		267
(-0.9) 		369
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 85.4
(3)
P 		23.9
(-2.7)
P 		21
(-3)
P 		56
(0.3)
P 		64
B 85.9
(3)
P 		30.4
(-1.5)
A 		12
(-3)
A 		77.8
(2.3)
P 		74
C 80.2
(3)
P 		31.4
(-1.7)
P 		18.5
(-3)
P 		68.5
(1.9)
P 		62
avr 83.8
(3) 		28.6
(-2) 		17.2
(-3) 		67.4
(1.5) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 21
(-3)
P 		55.7
(-1.2)
P 		80.7
(0.2)
P 		134.2
(3)
P 		125.9
(2.6)
P 		72
(-0.3)
P 		113
B 50.7
(-3)
P 		54.8
(-2.8)
P 		100.1
(-0.9)
P 		132.5
(0.4)
P 		193.5
(3)
P 		94.1
(-1.2)
P 		143
C 60.5
(-2.5)
P 		51.5
(-3)
P 		123.1
(1)
P 		100.4
(-0.3)
P 		160.1
(3)
P 		84.7
(-1.2)
P 		109
avr 44.1
(-3) 		54
(-2.5) 		101.3
(-0) 		122.4
(1.1) 		159.8
(3) 		83.6
(-1) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179815_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4838
Sequence Type Exemplar sequence
Representative Public ID T23F4.3 NCBI
Target Description T23F4.3 /REP_DB=WormBase Gene ID /WP=CE14088 /TR=O17263 /GB=AAB71032.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061524(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061524 NCBI Caenorhabditis elegans T23F4.3 (T23F4.3) mRNA, complete cds. 11/11 A 
SNAP00000024152 ENSEMBL cdna:SNAP chromosome:CEL140:II:1169895:1180906:-1 10/11 None
GENEFINDER00000024156 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1169846:1174611:-1 11/11 None
T23F4.3 ENSEMBL cdna:known chromosome:CEL140:II:1169846:1172090:-1 gene:T23F4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1169844-1172089(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T23F4.3
Entrez Gene 188808 Entrez gene
SwissProt O17263 EMBL-EBI
WormBase CE14088 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB71031 Nematode astacin protease protein 27 [Caenorhabditis elegans] ref|NP_493926.2| T23F4.4 [Caenorhabditis elegans] sp|O17264|NAS27_CAEEL Zinc metalloproteinase nas-27 precursor (Nematode astacin 27) 0.0
blast CAE62910 Hypothetical protein CBG07102 [Caenorhabditis briggsae] 0.0
blast AAB71032 Hypothetical protein T23F4.3 [Caenorhabditis elegans] ref|NP_493925.1| T23F4.3 [Caenorhabditis elegans] 1.0E-152
blast AAB71032 Hypothetical protein T23F4.3 [Caenorhabditis elegans] ref|NP_493925.1| T23F4.3 [Caenorhabditis elegans] 1.0E-125
blast CAE62911 Hypothetical protein CBG07103 [Caenorhabditis briggsae] 1.0E-121
blast AAB71033 Hypothetical protein T23F4.1 [Caenorhabditis elegans] ref|NP_493924.1| T23F4.1 [Caenorhabditis elegans] 1.0E-116
blast AAB71032 Hypothetical protein T23F4.3 [Caenorhabditis elegans] ref|NP_493925.1| T23F4.3 [Caenorhabditis elegans] 1.0E-115
Protein Domains
Database ID Description E-Value
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 6.9E-92
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 9.5E-90
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 5.7E-78
Pfam IPR005515 EMBL-EBI Vitelline membrane outer layer protein I (VOMI) 7.2E-8
Pfam IPR005515 EMBL-EBI Vitelline membrane outer layer protein I (VOMI) 3.5E-9
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 4.8E-86
Sequence
Target Sequence 
>C. ELEGANS:179815_AT
ggctaagctcggctatgtagcatttgctgcggatacctatggaaaaggaaaggccgctcc
agatgtgaatggaaattttgcaattatggggcaacttctaggcaatagaaccacaatcct
ccgcaaccgaattataagtgcttggacctatgtgaaggggctctcttttgtagatactaa
caatatcgggtcaattgggttctgttttggcgggctttgtactttggatttggcccgttt
caatgttggactaaaagctgctgtgtcgttccatggaacacttacggattacccgggaaa
tggaacagcaatcgacgcctcaattcaagctcatcatggagatgcagaccctcacactac
aaatgaaaatgccgaggatttcattgaagaaatgaggagaagaaacggtgattggatgtt
cagtagatacgcccacgccctccacgcctttactctccccggagtcgagaattggggaat
tccaggagccgcattcgatcctattgcttcgaataggtcttggagcgccatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTAAGCTCGGCTATGTAGCATTT 313 539 246 Antisense
GTAGCATTTGCTGCGGATACCTATG 452 457 262 Antisense
ATTATGGGGCAACTTCTAGGCAATA 486 13 328 Antisense
AGGCAATAGAACCACAATCCTCCGC 79 55 345 Antisense
CAATCCTCCGCAACCGAATTATAAG 34 181 359 Antisense
GGACCTATGTGAAGGGGCTCTCTTT 551 523 389 Antisense
GGGGCTCTCTTTTGTAGATACTAAC 19 499 402 Antisense
ATGGAACAGCAATCGACGCCTCAAT 680 51 545 Antisense
CTTTACTCTCCCCGGAGTCGAGAAT 693 217 693 Antisense
CCGCATTCGATCCTATTGCTTCGAA 149 263 734 Antisense
TTCGAATAGGTCTTGGAGCGCCATG 462 695 753 Antisense
Sequence Source Affymetrix Proprietary Database