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[179788_s_at]

Description T22B3.2A /REP_DB=WormBase Gene ID /WP=CE18277 /TR=Q21079 /GB=CAA92618.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 42      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 8.9
(-3)
A 		48.2
(3)
A 		44.5
(2.4)
A 		11.2
(-2.6)
A 		39
B 8.3
(-1.9)
A 		19.4
(3)
A 		5.7
(-3)
A 		14.3
(0.8)
A 		14
C 17.4
(1.1)
A 		22.4
(3)
A 		7
(-3)
A 		11.6
(-1.2)
A 		15
avr 11.5
(-3) 		30
(3) 		19.1
(-0.5) 		12.4
(-2.7) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 7.4
(-3)
A 		14.3
(-2.8)
A 		12.6
(-2.9)
A 		12.6
(-2.9)
A 		60.6
(-1.8)
A 		274.8
(3)
A 		267
B 12.6
(-2.8)
A 		15.6
(-2.7)
A 		6.5
(-3)
A 		20.6
(-2.6)
A 		119.8
(0.4)
A 		203.9
(3)
A 		197
C 15.8
(-2.8)
A 		10.6
(-3)
A 		18.5
(-2.7)
A 		30.6
(-2.3)
A 		129
(0.9)
A 		190.5
(3)
A 		180
avr 11.9
(-3) 		13.5
(-3) 		12.5
(-3) 		21.3
(-2.7) 		103.1
(-0.4) 		223.1
(3) 		211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 3.3
(-2)
A 		10.5
(2.3)
A 		11.6
(3)
A 		1.6
(-3)
A 		10
B 2.6
(-0.8)
A 		4.8
(3)
A 		1.3
(-3)
A 		3
(-0.1)
A 		4
C 5.8
(2.2)
A 		6.4
(3)
A 		2.4
(-2.5)
A 		2
(-3)
A 		4
avr 3.9
(-1) 		7.2
(3) 		5.1
(0.5) 		2.2
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 1.4
(-3)
A 		5.1
(-2.7)
A 		2.7
(-2.9)
A 		3.2
(-2.8)
A 		18.3
(-1.5)
A 		66.9
(3)
A 		66
B 4.4
(-2.8)
A 		5.2
(-2.7)
A 		1.6
(-3)
A 		5.5
(-2.7)
A 		48.7
(0.8)
A 		76
(3)
A 		74
C 5.9
(-2.7)
A 		3.2
(-3)
A 		4.7
(-2.9)
A 		6.2
(-2.7)
A 		41.8
(0.8)
A 		63.8
(3)
A 		61
avr 3.9
(-2.9) 		4.5
(-2.9) 		3
(-3) 		5
(-2.8) 		36.3
(0) 		68.9
(3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179788_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9004
Sequence Type Exemplar sequence
Representative Public ID T22B3.2A NCBI
Target Description T22B3.2A /REP_DB=WormBase Gene ID /WP=CE18277 /TR=Q21079 /GB=CAA92618.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069817(11), NM_069816(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069817 NCBI Caenorhabditis elegans T22B3.2a (T22B3.2) mRNA, complete cds. 11/11 None
NM_069816 NCBI Caenorhabditis elegans T22B3.2b (T22B3.2) mRNA, complete cds. 11/11 None
SNAP00000040609 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11706565:11708139:1 11/11 None
GENEFINDER00000040623 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11706538:11728967:1 11/11 None
T22B3.2a ENSEMBL cdna:known chromosome:CEL140:IV:11704335:11708207:1 gene:T22B3.2 11/11 None
T22B3.2b ENSEMBL cdna:known chromosome:CEL140:IV:11704335:11708139:1 gene:T22B3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11704340-11708145(+) 100.0 100.0
chrIII:9887188-9891219(-) 89.22 90.45
Public Domain and Genome References
Ensembl T22B3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92969 Hypothetical protein T22B3.2a [Caenorhabditis elegans] emb|CAA92618.1| Hypothetical protein T22B3.2a [Caenorhabditis elegans] ref|NP_502218.1| T22B3.2a [Caenorhabditis elegans] 0.0
blast CAA92970 Hypothetical protein T22B3.2b [Caenorhabditis elegans] emb|CAA92619.1| Hypothetical protein T22B3.2b [Caenorhabditis elegans] ref|NP_502217.1| T22B3.2b [Caenorhabditis elegans] 0.0
blast CAB54247 Hypothetical protein ZK757.3b [Caenorhabditis elegans] emb|CAB54514.1| Hypothetical protein ZK757.3b [Caenorhabditis elegans] ref|NP_499192.1| Temporarily Assigned Gene name family member (tag-76) [Caenorhabditis elegans] 0.0
blast CAA82941 Hypothetical protein ZK757.3a [Caenorhabditis elegans] emb|CAA82389.1| Hypothetical protein ZK757.3a [Caenorhabditis elegans] ref|NP_499191.1| Temporarily Assigned Gene name family member (tag-76) [Caenorhabditis elegans] sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromosome III 0.0
blast T23160 hypothetical protein K01A6.1 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 1.8E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 1.8E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 9.7E-8
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.2E-16
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 8.0E-15
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 5.7E-22
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-13
Pfam IPR008144 EMBL-EBI Guanylate kinase 2.4E-15
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:179788_S_AT
caatcaatccgtacggcttgcctggcaattgctgaagacttccgtccaccaataacctac
atcgttgttcaaaaaagacatcatgctcgcatcttctgcaagttcccgaacgacatggtt
ggaaaggcaaagaatgttccacctggtaccactgtcgacactggaatcgtctctcccgaa
ggatttgatttctacctatgctctcactatggagtacagggaacttctcgccctgcgaga
tatcacgttcttctggatgaatgcaagttcactgctgatgaaattcaaaacatcacttat
ggaatgtgccatacatatggtcgttgtactcgttcggtctccatcccaactccagtttac
tatgctgatttggttgctactcgtgcccgctgtcacatcaagagaaagctcggtcttgcc
gacaacaatgactgtgacaccaactcgctctcttcatcacttgcttctttgctcaacgtg
agaactggaagtggaaaaggaaagaagtcacatgctccaagcgtcgatgatgaatcgtat
tctcttcctgacgctgcatctgatcaaatccttcaggactgcgtctcggttgcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCAATCCGTACGGCTTGCCTGG 426 181 2485 Antisense
GTCCACCAATAACCTACATCGTTGT 314 477 2528 Antisense
ATCTTCTGCAAGTTCCCGAACGACA 539 31 2575 Antisense
GTACCACTGTCGACACTGGAATCGT 702 457 2630 Antisense
TTTCTACCTATGCTCTCACTATGGA 436 671 2673 Antisense
CCCTGCGAGATATCACGTTCTTCTG 114 261 2715 Antisense
GTGCCCGCTGTCACATCAAGAGAAA 320 479 2867 Antisense
TCACTTGCTTCTTTGCTCAACGTGA 444 627 2941 Antisense
GTCACATGCTCCAAGCGTCGATGAT 338 465 2991 Antisense
GACGCTGCATCTGATCAAATCCTTC 340 381 3034 Antisense
CTTCAGGACTGCGTCTCGGTTGCAG 35 221 3055 Antisense
Sequence Source Affymetrix Proprietary Database