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[179763_s_at]

Description R04E5.2 /REP_DB=WormBase Gene ID /WP=CE25966 /TR=Q9GYL2 /GB=AAG00012.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 90      6grp_total > 64      4grp_total > 01      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 151.7
(-1.6)
P 		134.5
(-3)
P 		193.9
(1.8)
P 		208.6
(3)
P 		74
B 127.3
(-1.9)
P 		142.8
(-1.4)
P 		91.3
(-3)
P 		285
(3)
P 		194
C 207.5
(2.1)
P 		106.3
(-3)
P 		181.2
(0.8)
P 		224.8
(3)
P 		119
avr 162.2
(-1.2) 		127.9
(-3) 		155.5
(-1.5) 		239.5
(3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 135.3
(-3)
P 		164.2
(0.1)
P 		173
(1)
P 		191.4
(3)
P 		153.9
(-1)
P 		141.5
(-2.3)
P 		56
B 123.3
(-3)
P 		142.7
(-1.8)
P 		197.9
(1.6)
P 		212.2
(2.5)
P 		150.7
(-1.3)
P 		221.1
(3)
P 		98
C 110.2
(-2.5)
P 		181.6
(1.7)
P 		162.3
(0.6)
P 		181.6
(1.7)
P 		100.7
(-3)
P 		204.4
(3)
P 		104
avr 122.9
(-3) 		162.8
(0.3) 		177.7
(1.6) 		195.1
(3) 		135.1
(-2) 		189
(2.5) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 55.2
(3)
P 		29.2
(-3)
P 		50.7
(2)
P 		30.5
(-2.7)
P 		26
B 40.6
(-0.1)
P 		35.3
(-0.9)
P 		21.2
(-3)
P 		60.7
(3)
P 		40
C 68.7
(3)
P 		30.6
(-3)
P 		63.1
(2.1)
P 		37.9
(-1.9)
P 		38
avr 54.8
(3) 		31.7
(-3) 		45
(0.5) 		43
(-0.1) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 24.9
(-3)
P 		58.4
(3)
P 		37.7
(-0.7)
P 		48.5
(1.2)
P 		46.4
(0.9)
P 		34.5
(-1.3)
P 		34
B 42.9
(-3)
P 		47.5
(-2.3)
P 		48.1
(-2.2)
P 		56.2
(-1)
P 		61.2
(-0.2)
P 		82.4
(3)
P 		40
C 40.8
(-1.6)
P 		55.4
(0.8)
P 		41.3
(-1.5)
P 		36.5
(-2.3)
P 		32.6
(-3)
P 		68.4
(3)
P 		36
avr 36.2
(-3) 		53.8
(1.1) 		42.4
(-1.5) 		47.1
(-0.4) 		46.7
(-0.5) 		61.8
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179763_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16530
Sequence Type Exemplar sequence
Representative Public ID R04E5.2 NCBI
Target Description R04E5.2 /REP_DB=WormBase Gene ID /WP=CE25966 /TR=Q9GYL2 /GB=AAG00012.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077092(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077092 NCBI Caenorhabditis elegans R04E5.2 (R04E5.2) mRNA, complete cds. 11/11 A 
R04E5.2 ENSEMBL cdna:known chromosome:CEL140:X:8812094:8816742:1 gene:R04E5.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000036374 8/11 Cross Hyb Matching Probes None
SNAP00000036369 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8812125-8816706(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R04E5.2
Entrez Gene 181129 Entrez gene
SwissProt Q9GYL2 EMBL-EBI
WormBase CE28746 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG00012 Hypothetical protein R04E5.2 [Caenorhabditis elegans] ref|NP_509493.1| R04E5.2 [Caenorhabditis elegans] 0.0
blast CAE69367 Hypothetical protein CBG15463 [Caenorhabditis briggsae] 0.0
blast T28874 hypothetical protein R04E5.2 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
scop a.4.2.Methylated DNA-protein cysteine methyltransferase, C-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; Methylated DNA-protein cysteine methyltransferase, C-terminal domain; Methylated DNA-protein cysteine methyltransferase, C-terminal domain 2.20000004768372
Pfam IPR002550 EMBL-EBI CBS 5.9E-48
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509493.1 4 154-176,204-226,231-253,260-282
R04E5.2 4 154-176,204-226,231-253,260-282
Sequence
Target Sequence 
>C. ELEGANS:179763_S_AT
agagccatcaactggcttcttcttccttcctgattacaatttgactgtcagtggaccatg
caggtttttgcaggtaactccgaaaatttcaataactagtgacgatgactttggatcacc
aaccagaaaagcatctatactggacagcagtccgaatagtagaaaacggtcgtcaacgag
tgtcatgaacagtttggcactacctactgcaagattggccgcgaaaatagcgtct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCCATCAACTGGCTTCTTCTTC 181 71 1980 Antisense
CCTTCCTGATTACAATTTGACTGTC 631 269 2004 Antisense
GACTGTCAGTGGACCATGCAGGTTT 78 375 2022 Antisense
GTGACGATGACTTTGGATCACCAAC 398 483 2078 Antisense
GGATCACCAACCAGAAAAGCATCTA 342 511 2092 Antisense
GAAAAGCATCTATACTGGACAGCAG 676 353 2105 Antisense
GGACAGCAGTCCGAATAGTAGAAAA 379 525 2121 Antisense
CGGTCGTCAACGAGTGTCATGAACA 566 243 2146 Antisense
GAACAGTTTGGCACTACCTACTGCA 491 347 2166 Antisense
CTACCTACTGCAAGATTGGCCGCGA 34 223 2179 Antisense
AAGATTGGCCGCGAAAATAGCGTCT 302 155 2190 Antisense
Sequence Source Affymetrix Proprietary Database