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[179762_at]

Description R04E5.2 /REP_DB=WormBase Gene ID /WP=CE25966 /TR=Q9GYL2 /GB=AAG00012.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 44      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 115.2
(3)
P 		109.3
(2.5)
A 		62.3
(-1.1)
A 		37.5
(-3)
M 		78
B 61.2
(-0.7)
A 		89.4
(3)
P 		43.1
(-3)
A 		52.3
(-1.8)
A 		46
C 70.9
(-3)
P 		88.5
(3)
A 		81.4
(0.6)
P 		88.4
(3)
A 		18
avr 82.4
(0.8) 		95.7
(3) 		62.3
(-2.5) 		59.4
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 66.9
(-0.7)
A 		75.3
(0.3)
P 		77.2
(0.5)
A 		98.6
(3)
P 		81.8
(1)
P 		47.2
(-3)
A 		51
B 73.9
(-0.1)
M 		54.2
(-3)
M 		86
(1.8)
P 		65.5
(-1.3)
A 		94.3
(3)
P 		77.6
(0.5)
P 		40
C 63.7
(-1)
M 		106.6
(2.9)
P 		41.7
(-3)
A 		108.1
(3)
A 		41.5
(-3)
A 		106.6
(2.9)
P 		67
avr 68.2
(-3) 		78.7
(-0.2) 		68.3
(-3) 		90.7
(3) 		72.5
(-1.9) 		77.1
(-0.6) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 41.9
(3)
P 		23.7
(0)
A 		16.3
(-1.2)
A 		5.5
(-3)
M 		36
B 19.5
(1.7)
A 		22.1
(3)
P 		10
(-3)
A 		11.1
(-2.5)
A 		12
C 23.5
(0.8)
P 		25.5
(1.7)
A 		28.4
(3)
P 		14.9
(-3)
A 		14
avr 28.3
(3) 		23.8
(1.5) 		18.2
(-0.4) 		10.5
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 12.3
(-2.7)
A 		26.8
(3)
P 		16.8
(-0.9)
A 		25
(2.3)
P 		24.7
(2.2)
P 		11.5
(-3)
A 		15
B 25.7
(-0.6)
M 		18
(-2.8)
M 		20.9
(-2)
P 		17.4
(-3)
A 		38.3
(3)
P 		28.9
(0.3)
P 		21
C 23.6
(0.1)
M 		32.5
(2.2)
P 		10.6
(-3)
A 		21.7
(-0.3)
A 		13.4
(-2.3)
A 		35.7
(3)
P 		25
avr 20.5
(-0.3) 		25.8
(3) 		16.1
(-3) 		21.4
(0.3) 		25.5
(2.8) 		25.4
(2.8) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179762_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16530
Sequence Type Exemplar sequence
Representative Public ID R04E5.2 NCBI
Target Description R04E5.2 /REP_DB=WormBase Gene ID /WP=CE25966 /TR=Q9GYL2 /GB=AAG00012.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077092(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077092 NCBI Caenorhabditis elegans R04E5.2 (R04E5.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000036374 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8812232:8816707:1 11/11 None
SNAP00000036369 ENSEMBL cdna:SNAP chromosome:CEL140:X:8815719:8816707:1 9/11 None
R04E5.2 ENSEMBL cdna:known chromosome:CEL140:X:8812094:8816742:1 gene:R04E5.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036368 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8812125-8816706(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R04E5.2
Entrez Gene 181129 Entrez gene
SwissProt Q9GYL2 EMBL-EBI
WormBase CE28746 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG00012 Hypothetical protein R04E5.2 [Caenorhabditis elegans] ref|NP_509493.1| R04E5.2 [Caenorhabditis elegans] 0.0
blast CAE69367 Hypothetical protein CBG15463 [Caenorhabditis briggsae] 0.0
blast T28874 hypothetical protein R04E5.2 - Caenorhabditis elegans 0.0
blast AAG00012 Hypothetical protein R04E5.2 [Caenorhabditis elegans] ref|NP_509493.1| R04E5.2 [Caenorhabditis elegans] 2.0E-79
Protein Domains
Database ID Description E-Value
scop a.4.2.Methylated DNA-protein cysteine methyltransferase, C-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; Methylated DNA-protein cysteine methyltransferase, C-terminal domain; Methylated DNA-protein cysteine methyltransferase, C-terminal domain 2.20000004768372
Pfam IPR002550 EMBL-EBI CBS 5.9E-48
Pfam IPR002550 EMBL-EBI CBS 1.7E-47
Pfam IPR000644 EMBL-EBI CBS domain 0.02
Pfam IPR000644 EMBL-EBI CBS domain 0.49
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509493.1 4 154-176,204-226,231-253,260-282
R04E5.2 4 154-176,204-226,231-253,260-282
GENEFINDER00000036374 3 141-163,218-240,247-269
Sequence
Target Sequence 
>C. ELEGANS:179762_AT
gaagagtgagcgtttgtcacttcatatgctggagatgaccgaaaaatggttgctagaaaa
gacaccattatttggaaatatgaacccaaaggcttttgaaaacctgattcaaagaaatat
cagagaggttctaattgtgcctccaaaaaatagcacgtccccgggaacattaaacctttt
tgaagctggtgttatgagtaagcgatttttattgattttggaaggaaaagcaacaatccg
ctttaatgagaaagacctcatctttgaatgcggaccttggacttgttttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGTGAGCGTTTGTCACTTCAT 398 339 1644 Antisense
GCGTTTGTCACTTCATATGCTGGAG 624 291 1653 Antisense
GGAAATATGAACCCAAAGGCTTTTG 311 527 1717 Antisense
CAAAGGCTTTTGAAAACCTGATTCA 516 191 1730 Antisense
GAGAGGTTCTAATTGTGCCTCCAAA 50 395 1766 Antisense
GCCTCCAAAAAATAGCACGTCCCCG 637 281 1782 Antisense
GGAACATTAAACCTTTTTGAAGCTG 10 529 1807 Antisense
GAAGCTGGTGTTATGAGTAAGCGAT 576 341 1825 Antisense
AGCAACAATCCGCTTTAATGAGAAA 233 77 1872 Antisense
AGACCTCATCTTTGAATGCGGACCT 445 69 1896 Antisense
AATGCGGACCTTGGACTTGTTTTGG 434 163 1910 Antisense
Sequence Source Affymetrix Proprietary Database