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[179757_at]

Description C25A8.2 /REP_DB=WormBase Gene ID /WP=CE08383 /TR=Q18144 /GB=AAB03180.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 8.5
(-1.3)
A 		3.1
(-2.5)
A 		1
(-3)
A 		27.2
(3)
A 		26
B 5.1
(-3)
A 		8.1
(-1)
A 		10.8
(0.9)
A 		13.9
(3)
A 		9
C 1.7
(-2.9)
A 		5.6
(-2.6)
A 		1.1
(-3)
A 		65.9
(3)
A 		65
avr 5.1
(-2.8) 		5.6
(-2.8) 		4.3
(-3) 		35.7
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 12.2
(0.9)
A 		1.3
(-3)
A 		18.2
(3)
A 		2.2
(-2.7)
A 		4.4
(-1.9)
A 		17.8
(2.9)
A 		17
B 21.7
(1.3)
A 		5.1
(-2.2)
A 		1.2
(-3)
A 		5.3
(-2.1)
A 		19
(0.7)
A 		29.8
(3)
A 		29
C 2.7
(-2.8)
A 		16.1
(0.1)
A 		19.7
(0.9)
A 		1.7
(-3)
A 		29.2
(3)
A 		8.4
(-1.5)
A 		28
avr 12.2
(0.5) 		7.5
(-1.3) 		13
(0.8) 		3.1
(-3) 		17.5
(2.5) 		18.7
(3) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 3.1
(1.5)
A 		0.7
(-2.4)
A 		0.3
(-3)
A 		4
(3)
A 		4
B 1.6
(-3)
A 		2
(-1.3)
A 		2.5
(0.9)
A 		3
(3)
A 		1
C 0.6
(-2.9)
A 		1.6
(-2.3)
A 		0.4
(-3)
A 		11.1
(3)
A 		11
avr 1.8
(-2.1) 		1.4
(-2.6) 		1.1
(-3) 		6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 2.3
(-0.2)
A 		0.5
(-3)
A 		4
(2.5)
A 		0.6
(-2.8)
A 		1.3
(-1.7)
A 		4.3
(3)
A 		4
B 7.5
(1)
A 		1.7
(-2.2)
A 		0.3
(-3)
A 		1.4
(-2.4)
A 		7.7
(1.1)
A 		11.1
(3)
A 		11
C 1
(-2.5)
A 		4.9
(0)
A 		5
(0.1)
A 		0.3
(-3)
A 		9.4
(3)
A 		2.8
(-1.4)
A 		9
avr 3.6
(0.2) 		2.4
(-1.2) 		3.1
(-0.4) 		0.8
(-3) 		6.1
(3) 		6.1
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179757_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9974
Sequence Type Exemplar sequence
Representative Public ID C25A8.2 NCBI
Target Description C25A8.2 /REP_DB=WormBase Gene ID /WP=CE08383 /TR=Q18144 /GB=AAB03180.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068682, NM_074191
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
R02D5.7 ENSEMBL cdna:known chromosome:CEL140:V:14483005:14483853:-1 gene:R02D5.7 None
NM_074191 NCBI Caenorhabditis elegans R02D5.7 (R02D5.7) mRNA, complete cds. None
GENEFINDER00000007924 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14483005:14484314:-1 None
SNAP00000010524 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7010257:7010728:1 None
GENEFINDER00000010526 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7010257:7010728:1 None
SNAP00000007912 ENSEMBL cdna:SNAP chromosome:CEL140:V:14483005:14483449:-1 None
C25A8.2 ENSEMBL cdna:known chromosome:CEL140:IV:7009825:7010728:1 gene:C25A8.2 None
NM_068682 NCBI Caenorhabditis elegans C25A8.2 (C25A8.2) mRNA, complete cds. None
NM_074191 NCBI Caenorhabditis elegans R02D5.7 (R02D5.7) mRNA, complete cds. None
R02D5.7 ENSEMBL cdna:known chromosome:CEL140:V:14483005:14483853:-1 gene:R02D5.7 None
C25A8.2 ENSEMBL cdna:known chromosome:CEL140:IV:7009825:7010728:1 gene:C25A8.2 None
SNAP00000010524 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7010257:7010728:1 None
NM_068682 NCBI Caenorhabditis elegans C25A8.2 (C25A8.2) mRNA, complete cds. None
GENEFINDER00000010526 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7010257:7010728:1 None
SNAP00000007912 ENSEMBL cdna:SNAP chromosome:CEL140:V:14483005:14483449:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7010028-7010734(+) 100.0 100.0
chrV:14483004-14483686(-) 92.37 92.37
Public Domain and Genome References
Gene Title

Ensembl C25A8.2
R02D5.7
Entrez Gene 177465 Entrez gene
179954 Entrez gene
SwissProt Q18144 EMBL-EBI
O62323 EMBL-EBI
WormBase CE31313 Wormbase
CE29435 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB03180 Hypothetical protein C25A8.2 [Caenorhabditis elegans] ref|NP_501083.2| C25A8.2 [Caenorhabditis elegans] 4.0E-40
blast CAB01436 Hypothetical protein R02D5.7 [Caenorhabditis elegans] ref|NP_506592.1| R02D5.7 [Caenorhabditis elegans] 2.0E-37
blast CAB01436 Hypothetical protein R02D5.7 [Caenorhabditis elegans] ref|NP_506592.1| R02D5.7 [Caenorhabditis elegans] 2.0E-34
blast AAB03180 Hypothetical protein C25A8.2 [Caenorhabditis elegans] ref|NP_501083.2| C25A8.2 [Caenorhabditis elegans] 3.0E-33
blast AAB03180 Hypothetical protein C25A8.2 [Caenorhabditis elegans] ref|NP_501083.2| C25A8.2 [Caenorhabditis elegans] 2.0E-24
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501083.2 1 10-32
NP_506592.1 1 10-32
C25A8.2 1 10-32
R02D5.7 1 10-32
Sequence
Target Sequence 
>C. ELEGANS:179757_AT
tgctctccagaaattagcaactcaaagatcaattggctaaaagtagataaaaaatattcg
aaaaacgcagaaaatttgctgccaaattctccccatttctgccaagtttcatgcaaaaaa
aacaatattttctttgagttcgatgtctcttcgttgtgccaatttgttgttcaactcctc
caattctgcaatgtttttattgttctcgcagtgatgaccatcacctcatgcggaaagaaa
aaagcatcaaatagcaaggaaaattgcaaaaagagtttgcaaaccggccctggagcagcc
accgaagccggagctgcttcttcgttagctccggttgacgcgaccaagcttgccacacct
gtaccagcggcgccaaaaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCTCCAGAAATTAGCAACTCAA 147 581 34 Antisense
AGCAACTCAAAGATCAATTGGCTAA 701 73 49 Antisense
AAAAACGCAGAAAATTTGCTGCCAA 494 129 94 Antisense
CCCCATTTCTGCCAAGTTTCATGCA 688 261 124 Antisense
CAATATTTTCTTTGAGTTCGATGTC 332 181 156 Antisense
TCTTCGTTGTGCCAATTTGTTGTTC 522 617 181 Antisense
GTTGTGCCAATTTGTTGTTCAACTC 603 437 186 Antisense
AATGTTTTTATTGTTCTCGCAGTGA 43 165 223 Antisense
ATTGTTCTCGCAGTGATGACCATCA 228 5 232 Antisense
GCAGTGATGACCATCACCTCATGCG 280 311 241 Antisense
ACCTGTACCAGCGGCGCCAAAAAAG 625 87 390 Antisense
Sequence Source Affymetrix Proprietary Database