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[179724_at]

Description F53C3.4 /REP_DB=WormBase Gene ID /WP=CE19442 /TR=Q9TXT5 /GB=AAC67455.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 38      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 96.1
(3)
A 		27.8
(-1.7)
A 		9
(-3)
A 		32
(-1.4)
A 		87
B 66.2
(3)
A 		39.5
(0.3)
A 		7.4
(-3)
A 		41.9
(0.5)
A 		59
C 92
(3)
P 		52.6
(0)
A 		17.8
(-2.6)
A 		12.1
(-3)
A 		80
avr 84.8
(3) 		40
(-0.7) 		11.4
(-3) 		28.7
(-1.6) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 89.8
(3)
A 		6.1
(-2.8)
A 		16
(-2.2)
A 		3.9
(-3)
A 		12.6
(-2.4)
A 		8
(-2.7)
A 		86
B 52.2
(3)
A 		19.1
(-1.2)
A 		11
(-2.2)
A 		5.4
(-2.9)
A 		4.8
(-3)
A 		24.2
(-0.5)
A 		47
C 93.9
(3)
M 		55.9
(0.4)
A 		6.4
(-3)
A 		12.8
(-2.5)
A 		6.2
(-3)
A 		31.8
(-1.2)
A 		88
avr 78.6
(3) 		27
(-1.3) 		11.1
(-2.7) 		7.4
(-3) 		7.9
(-3) 		21.3
(-1.8) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 35
(3)
A 		6
(-2.3)
A 		2.4
(-3)
A 		4.7
(-2.6)
A 		33
B 21.1
(3)
A 		9.7
(-0.5)
A 		1.7
(-3)
A 		8.9
(-0.8)
A 		19
C 30.4
(3)
P 		15.1
(-0.2)
A 		6.2
(-2.1)
A 		2
(-3)
A 		28
avr 28.8
(3) 		10.3
(-1.4) 		3.4
(-3) 		5.2
(-2.6) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 16.5
(3)
A 		2.2
(-2.5)
A 		3.5
(-2)
A 		1
(-3)
A 		3.8
(-1.9)
A 		2
(-2.6)
A 		16
B 18.2
(3)
A 		6.4
(-1.2)
A 		2.7
(-2.5)
A 		1.4
(-3)
A 		2
(-2.8)
A 		9
(-0.3)
A 		17
C 34.8
(3)
M 		17.1
(-0.2)
A 		1.6
(-3)
A 		2.6
(-2.8)
A 		2
(-2.9)
A 		10.6
(-1.4)
A 		33
avr 23.2
(3) 		8.6
(-1.1) 		2.6
(-2.7) 		1.7
(-3) 		2.6
(-2.7) 		7.2
(-1.5) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179724_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5539
Sequence Type Exemplar sequence
Representative Public ID F53C3.4 NCBI
Target Description F53C3.4 /REP_DB=WormBase Gene ID /WP=CE19442 /TR=Q9TXT5 /GB=AAC67455.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062289(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062289 NCBI Caenorhabditis elegans F53C3.4 (F53C3.4) mRNA, complete cds. 9/11 None
SNAP00000027223 ENSEMBL cdna:SNAP chromosome:CEL140:II:3901420:3902518:1 9/11 None
GENEFINDER00000027232 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3901420:3902518:1 11/11 None
F53C3.4 ENSEMBL cdna:known chromosome:CEL140:II:3901401:3902734:1 gene:F53C3.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3901418-3902517(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53C3.4
Entrez Gene 259429 Entrez gene
SwissProt Q9TXT5 EMBL-EBI
WormBase CE30143 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast B88115 protein F53C3.4 [imported] - Caenorhabditis elegans 1.0E-129
blast AAC67455 Hypothetical protein F53C3.4 [Caenorhabditis elegans] ref|NP_494690.2| F53C3.4 [Caenorhabditis elegans] 1.0E-116
blast B88115 protein F53C3.4 [imported] - Caenorhabditis elegans 1.0E-113
blast AAC67455 Hypothetical protein F53C3.4 [Caenorhabditis elegans] ref|NP_494690.2| F53C3.4 [Caenorhabditis elegans] 1.0E-113
blast AAC67454 Hypothetical protein F53C3.3 [Caenorhabditis elegans] ref|NP_494691.2| F53C3.3 [Caenorhabditis elegans] 1.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 1.9E-28
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 1.9E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494690.2 1 150-169
F53C3.4 1 150-169
SNAP00000027223 1 150-169
GENEFINDER00000027232 1 170-189
Sequence
Target Sequence 
>C. ELEGANS:179724_AT
ggtcgtcgttatatcgcgagtttccggcagaatctattccatatcagatcaactgccaag
ttattttcatattctccgataactcgtactcatgtcatcatatcacctgcttctccgata
attcacctaaaagtgggatacggcaacatatatataacctatattctatgttttcctttc
agttacggagattaccattactattgggaagggcatcatgtttatactacacaaaaacct
gacaagtgtgattatcaaataatcgataaggataaggaattgagaaatataacattcgca
aatggcacaagaccagcaattatcacctttggatgtgcagagtatacttattgttgtgga
ttggaatgttgtactggtttaagtcagttagaggaaggattatttttaattggagtttca
ctggcggtactttttctcgcggtgatcaccgttttgttggacaaatgtaaaaagagaacg
actaaacgattcaatcttccaagatcaactccagaagttcaagaactgactcccagtgct
cctaatgctacaaacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCGTCGTTATATCGCGAGTTTCC 281 501 121 Antisense
GTTTCCGGCAGAATCTATTCCATAT 518 451 140 Antisense
ATTTTCATATTCTCCGATAACTCGT 452 13 183 Antisense
GATAACTCGTACTCATGTCATCATA 433 423 198 Antisense
CTGCTTCTCCGATAATTCACCTAAA 597 237 227 Antisense
TATTCTATGTTTTCCTTTCAGTTAC 58 665 282 Antisense
GGCACAAGACCAGCAATTATCACCT 474 535 424 Antisense
GAGTTTCACTGGCGGTACTTTTTCT 362 399 533 Antisense
TACTTTTTCTCGCGGTGATCACCGT 202 645 548 Antisense
GGTGATCACCGTTTTGTTGGACAAA 429 499 561 Antisense
CCAGTGCTCCTAATGCTACAAACAA 625 271 653 Antisense
Sequence Source Affymetrix Proprietary Database