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[179708_s_at]

Description F25G6.9 /REP_DB=WormBase Gene ID /WP=CE09645 /TR=O16928 /GB=AAC25802.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_highest_8_8 > 26      4grp_high_8_8 > 57      6grp_highest_nonorm > 82      6grp_total > 66      4grp_total > 63      6grp_highest_sub > Da      6grp_high1_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 344.8
(0.8)
P 		343.9
(0.7)
P 		302.3
(-3)
P 		368.9
(3)
P 		67
B 427.5
(1.9)
P 		410.3
(1.4)
P 		463.6
(3)
P 		266.5
(-3)
P 		197
C 333.5
(0.3)
P 		275.1
(-3)
P 		382.1
(3)
P 		346.5
(1)
P 		107
avr 368.6
(1.5) 		343.1
(-1.3) 		382.7
(3) 		327.3
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 234.6
(-3)
P 		458.6
(3)
P 		374.5
(0.7)
P 		360.2
(0.4)
P 		309.9
(-1)
P 		416.5
(1.9)
P 		224
B 325.6
(-0.5)
P 		315.9
(-0.7)
P 		419.9
(1.4)
P 		301.1
(-1)
P 		202.5
(-3)
P 		496
(3)
P 		294
C 331.1
(-2.2)
P 		334.3
(-2.1)
P 		306.3
(-3)
P 		376.8
(-0.8)
P 		417.6
(0.5)
P 		496.6
(3)
P 		190
avr 297.1
(-3) 		369.6
(-0.5) 		366.9
(-0.6) 		346
(-1.3) 		310
(-2.6) 		469.7
(3) 		173
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 125.5
(3)
P 		74.7
(-1.3)
P 		79
(-0.9)
P 		54
(-3)
P 		72
B 136.3
(3)
P 		101.3
(0.4)
P 		107.4
(0.8)
P 		56.8
(-3)
P 		80
C 110.4
(1.2)
P 		79.1
(-1.3)
P 		133.2
(3)
P 		58.4
(-3)
P 		75
avr 124.1
(3) 		85
(-0.5) 		106.5
(1.4) 		56.4
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 43.2
(-3)
P 		163.1
(3)
P 		81.6
(-1.1)
P 		91.4
(-0.6)
P 		93.5
(-0.5)
P 		101.4
(-0.1)
P 		120
B 113.3
(-1.1)
P 		105.2
(-1.5)
P 		102
(-1.7)
P 		79.7
(-3)
P 		82.3
(-2.9)
P 		184.9
(3)
P 		105
C 122.7
(0.1)
P 		102
(-1.3)
P 		78
(-2.9)
P 		75.8
(-3)
P 		135.1
(0.9)
P 		166.2
(3)
P 		90
avr 93.1
(-2.1) 		123.4
(0.6) 		87.2
(-2.6) 		82.3
(-3) 		103.6
(-1.1) 		150.8
(3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179708_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12225
Sequence Type Exemplar sequence
Representative Public ID F25G6.9 NCBI
Target Description F25G6.9 /REP_DB=WormBase Gene ID /WP=CE09645 /TR=O16928 /GB=AAC25802.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072808(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072808 NCBI Caenorhabditis elegans F25G6.9 (F25G6.9) mRNA, complete cds. 11/11 None
SNAP00000003982 ENSEMBL cdna:SNAP chromosome:CEL140:V:8567997:8572543:-1 11/11 None
GENEFINDER00000003988 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8567997:8572543:-1 11/11 None
F25G6.9 ENSEMBL cdna:known chromosome:CEL140:V:8567885:8572564:-1 gene:F25G6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8567997-8572544(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25G6.9
Entrez Gene 179237 Entrez gene
SwissProt O16928 EMBL-EBI
WormBase CE32879 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25802 Hypothetical protein F25G6.9 [Caenorhabditis elegans] ref|NP_505209.2| F25G6.9 [Caenorhabditis elegans] 0.0
blast CAE58365 Hypothetical protein CBG01490 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:179708_S_AT
agatccagacgcattcggaggtcaaatttccaactgctttgatgtatggttggaagctcg
cagaggtgaaaagatgaacatcagaagaatttgtgacatttttggcaaggaggagagtaa
ttcgaagaagaatgcgatttgtgcattgatctctcacgaatcaagaacactttcaaggaa
gatgagatttgaaaagcttctcgacgtgattactgcaagccgcatcgcaagaacattcgg
cggatctatgcaatcattcttttccatctttctttcaatcatcgtcagcaacaatgatac
tgtcgaaattccattgtgttggtttgaaaatcaagaacatgttcctgcgatgatgatcgc
tgctaagcaaccattttacaaatggattgttgaagctccagaaatgaaatccaatgctca
ccatttcgggtcatttttgtcgtattatgtggatgggccggaaaaacaatattacgaata
ttggcacacagaaggggcaacagacttgctcgccatgattgtctctgcctacccaaatga
gaccttcccgaaccttcttactcaaaccgatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATCCAGACGCATTCGGAGGTCAA 291 67 3378 Antisense
GATTTGTGCATTGATCTCTCACGAA 596 427 3513 Antisense
CTCGACGTGATTACTGCAAGCCGCA 71 233 3577 Antisense
GCAAGCCGCATCGCAAGAACATTCG 487 319 3592 Antisense
GAACATTCGGCGGATCTATGCAATC 408 351 3608 Antisense
TCTTTCTTTCAATCATCGTCAGCAA 641 615 3644 Antisense
GAACATGTTCCTGCGATGATGATCG 370 351 3712 Antisense
GATGATCGCTGCTAAGCAACCATTT 465 411 3729 Antisense
AGACTTGCTCGCCATGATTGTCTCT 578 71 3879 Antisense
TCTCTGCCTACCCAAATGAGACCTT 558 611 3899 Antisense
CGAACCTTCTTACTCAAACCGATGA 200 253 3926 Antisense
Sequence Source Affymetrix Proprietary Database