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[179689_at]

Description F35A5.4 /REP_DB=WormBase Gene ID /WP=CE04488 /TR=Q20000 /GB=AAB52645.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 49      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 255
1 2 3 4 range
A 16.2
(-3)
A 		157.3
(0.5)
A 		260.8
(3)
A 		32.9
(-2.6)
A 		245
B 11
(-3)
A 		259.5
(3)
M 		205.9
(1.7)
P 		30.4
(-2.5)
A 		249
C 14.3
(-2.9)
A 		264.3
(3)
A 		212.6
(1.8)
P 		9.3
(-3)
A 		255
avr 13.8
(-3) 		227
(3) 		226.4
(3) 		24.2
(-2.7) 		213

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 154
1 2 3 4 5 6 range
A 14
(-3)
A 		119.5
(1.5)
A 		64.1
(-0.9)
A 		61.8
(-1)
A 		103.8
(0.8)
A 		155.9
(3)
A 		142
B 6.4
(-3)
A 		87.5
(0.2)
A 		39.6
(-1.7)
A 		74.2
(-0.4)
A 		98.3
(0.6)
A 		160.3
(3)
P 		154
C 9.2
(-3)
A 		90
(0.6)
A 		65.6
(-0.5)
A 		58.1
(-0.8)
A 		122.8
(2.1)
A 		143.7
(3)
A 		135
avr 9.9
(-3) 		99
(0.7) 		56.4
(-1.1) 		64.7
(-0.7) 		108.3
(1.1) 		153.3
(3) 		143
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 5.9
(-2.9)
A 		34.2
(-0.2)
A 		68.2
(3)
A 		4.8
(-3)
A 		63
B 3.5
(-3)
A 		64.1
(3)
M 		47.7
(1.4)
P 		6.5
(-2.7)
A 		61
C 4.7
(-2.8)
A 		76
(3)
A 		74.1
(2.8)
P 		1.6
(-3)
A 		74
avr 4.7
(-3) 		58.1
(2.5) 		63.3
(3) 		4.3
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 2.6
(-3)
A 		42.5
(3)
A 		14
(-1.3)
A 		15.7
(-1)
A 		31.3
(1.3)
A 		37.9
(2.3)
A 		40
B 2.2
(-3)
A 		29.1
(-0.2)
A 		9.6
(-2.2)
A 		19.6
(-1.2)
A 		39.9
(0.9)
A 		59.8
(3)
P 		58
C 3.4
(-3)
A 		27.5
(0.2)
A 		16.7
(-1.2)
A 		11.7
(-1.9)
A 		39.7
(1.9)
A 		48.1
(3)
A 		45
avr 2.7
(-3) 		33
(1) 		13.4
(-1.6) 		15.7
(-1.3) 		37
(1.5) 		48.6
(3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179689_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18229
Sequence Type Exemplar sequence
Representative Public ID F35A5.4 NCBI
Target Description F35A5.4 /REP_DB=WormBase Gene ID /WP=CE04488 /TR=Q20000 /GB=AAB52645.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076256(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076256 NCBI Caenorhabditis elegans F35A5.4 (F35A5.4) mRNA, complete cds. 10/11 None
SNAP00000032201 ENSEMBL cdna:SNAP chromosome:CEL140:X:3789869:3792790:-1 10/11 None
GENEFINDER00000032209 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3789869:3795353:-1 10/11 None
F35A5.4 ENSEMBL cdna:known chromosome:CEL140:X:3789741:3792799:-1 gene:F35A5.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3791241-3792791(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35A5.4
Entrez Gene 185249 Entrez gene
SwissProt Q20000 EMBL-EBI
WormBase CE37642 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.22268.1.S1_X_AT similar to keratin associated protein 10-7 cfa
CANINE_2:CFAAFFX.22273.1.S1_S_AT similar to keratin associated protein 10-7 cfa
CANINE_2:CFAAFFX.22273.1.S1_X_AT similar to keratin associated protein 10-7 cfa
CANINE_2:CFAAFFX.23307.1.S1_AT similar to keratin associated protein 10-7 cfa
Protein Similarities
Method ID Description E-Value
blast AAB52645 Hypothetical protein F35A5.4 [Caenorhabditis elegans] ref|NP_508657.2| F35A5.4 [Caenorhabditis elegans] 0.0
blast CAE68483 Hypothetical protein CBG14285 [Caenorhabditis briggsae] 0.0
blast AAA96110 Prion-like-(q/n-rich)-domain-bearing protein protein 13 [Caenorhabditis elegans] ref|NP_504494.2| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-13) [Caenorhabditis elegans] 8.0E-59
blast AAA96110 Prion-like-(q/n-rich)-domain-bearing protein protein 13 [Caenorhabditis elegans] ref|NP_504494.2| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-13) [Caenorhabditis elegans] 3.0E-57
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032209 1 47-69
Sequence
Target Sequence 
>C. ELEGANS:179689_AT
gtcccgatcggtgtcaaccaatgtgcacgcaagcttgtgttatggctaagacctcagttt
gtgtggatcaatgtatgccaaaatgcgattcagcatgtataaatttaggaccatcttgtg
accagcagtgtatgccattatgtctcccagcttgtattaacgccatccaaggaccgacgg
agtgtgctccacagtgcatgccatcatgctcatcaaattgtattcagcaagtattcccga
gctgtcctcaacaatgtcaaccggtgtgcactccacaatgtatccagtcaatccaagtag
caattcaacgtccaacttgtgcttcatcgtgcatgccttcctgctcccaatcatgcatcc
agaaatacgaaattaccgtcgagcaagagacttgtgttccagcatgtatgccagcatgct
catcggcttgcgttcaagcagtcacatgcagtacctgtaccaataattgcccatctatct
gctcgcaagcgaactgcattccacagtgtatgccaagatgcctccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCCGATCGGTGTCAACCAATGTG 647 473 206 Antisense
TTAGGACCATCTTGTGACCAGCAGT 610 677 310 Antisense
GCAGTGTATGCCATTATGTCTCCCA 598 311 330 Antisense
TGTCTCCCAGCTTGTATTAACGCCA 75 557 346 Antisense
AACGTCCAACTTGTGCTTCATCGTG 194 145 512 Antisense
GAGACTTGTGTTCCAGCATGTATGC 534 393 592 Antisense
TGTATGCCAGCATGCTCATCGGCTT 469 559 610 Antisense
GCAGTCACATGCAGTACCTGTACCA 627 311 643 Antisense
CCAATAATTGCCCATCTATCTGCTC 325 277 665 Antisense
TGCTCGCAAGCGAACTGCATTCCAC 414 581 685 Antisense
CACAGTGTATGCCAAGATGCCTCCC 641 195 707 Antisense
Sequence Source Affymetrix Proprietary Database