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[179650_at]

Description K08D8.4 /REP_DB=WormBase Gene ID /WP=CE06143 /TR=Q21330 /GB=CAA97435.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 61      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 119.8
(2.5)
P 		70.2
(-0.1)
A 		129.2
(3)
P 		13.9
(-3)
A 		115
B 88
(1.6)
P 		87.4
(1.5)
A 		110.1
(3)
P 		18
(-3)
A 		92
C 105.2
(1.8)
M 		65.7
(-0.6)
A 		126.5
(3)
P 		24.1
(-3)
A 		102
avr 104.3
(2) 		74.4
(0.2) 		121.9
(3) 		18.7
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 42.5
(-1.5)
A 		14.3
(-2.7)
A 		35.9
(-1.8)
A 		6
(-3)
A 		61.3
(-0.8)
A 		155.5
(3)
P 		150
B 15.8
(-2.6)
A 		53.2
(1.2)
A 		12
(-3)
A 		35.2
(-0.6)
A 		38.2
(-0.3)
A 		71.1
(3)
P 		59
C 19.8
(-2.4)
A 		27.9
(-2.1)
A 		37.1
(-1.7)
A 		65.7
(-0.4)
A 		6
(-3)
A 		146.2
(3)
P 		140
avr 26
(-3) 		31.8
(-2.6) 		28.3
(-2.9) 		35.6
(-2.4) 		35.2
(-2.4) 		124.3
(3) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 43.6
(3)
P 		15.2
(-1.1)
A 		33.8
(1.6)
P 		2
(-3)
A 		42
B 28.1
(3)
P 		21.6
(1.4)
A 		25.5
(2.4)
P 		3.8
(-3)
A 		24
C 34.8
(1.6)
M 		18.9
(-0.8)
A 		44.1
(3)
P 		4.1
(-3)
A 		40
avr 35.5
(3) 		18.6
(-0.1) 		34.5
(2.8) 		3.3
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 7.8
(-2)
A 		5.1
(-2.4)
A 		7.8
(-2)
A 		1.5
(-3)
A 		18.5
(-0.2)
A 		37.9
(3)
P 		36
B 5.5
(-2.3)
A 		17.7
(0.8)
A 		2.9
(-3)
A 		9.3
(-1.4)
A 		15.5
(0.2)
A 		26.5
(3)
P 		24
C 7.3
(-2.3)
A 		8.5
(-2.2)
A 		9.4
(-2)
A 		13.2
(-1.6)
A 		1.9
(-3)
A 		49
(3)
P 		47
avr 6.9
(-3) 		10.4
(-2.3) 		6.7
(-3) 		8
(-2.7) 		12
(-2) 		37.8
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179650_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9377
Sequence Type Exemplar sequence
Representative Public ID K08D8.4 NCBI
Target Description K08D8.4 /REP_DB=WormBase Gene ID /WP=CE06143 /TR=Q21330 /GB=CAA97435.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028113(11), NM_001028116(11), NM_001028115(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028113 NCBI Caenorhabditis elegans K08D8.4a (K08D8.4) mRNA, complete cds. 11/11 None
NM_001028116 NCBI Caenorhabditis elegans K08D8.4d (K08D8.4) mRNA, complete cds. 11/11 None
NM_001028115 NCBI Caenorhabditis elegans K08D8.4c (K08D8.4) mRNA, complete cds. 10/11 None
SNAP00000040292 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12907517:12909949:-1 11/11 None
GENEFINDER00000040298 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12904234:12912079:-1 11/11 None
K08D8.4d ENSEMBL cdna:known chromosome:CEL140:IV:12907466:12909949:-1 gene:K08D8.4 11/11 None
K08D8.4a ENSEMBL cdna:known chromosome:CEL140:IV:12907466:12909949:-1 gene:K08D8.4 11/11 None
K08D8.4c ENSEMBL cdna:known chromosome:CEL140:IV:12907467:12909968:-1 gene:K08D8.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028114 5/11 Cross Hyb Matching Probes None
K08D8.4b 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
187144 4 3 NM_001028113 179650_at (11)
NM_001028115 179650_at (10)
NM_001028116 179650_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12907806-12909955(-) 100.0 100.0
Public Domain and Genome References
Ensembl K08D8.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA97435 Hypothetical protein K08D8.4a [Caenorhabditis elegans] ref|NP_001023284.1| K08D8.4a [Caenorhabditis elegans] 0.0
blast CAI59116 Hypothetical protein K08D8.4d [Caenorhabditis elegans] ref|NP_001023287.1| K08D8.4d [Caenorhabditis elegans] 0.0
blast CAI59115 Hypothetical protein K08D8.4c [Caenorhabditis elegans] ref|NP_001023286.1| K08D8.4c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.9E-47
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 4.5E-47
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.9E-47
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 2.4E-38
Sequence
Target Sequence 
>C. ELEGANS:179650_AT
agaaaaacgtgtctgcggtggccactatttcgagacaacctgattacttgactcttccga
agggatttgcaaatagcatcacactaaaaatttacggagaaactatggaagaaagcaacc
tttttgccacaatcaactccaactaccagtcaatgttcccactaagagtcacaaattccc
ttaaaatctatcactcagataccggattacttacggttccagttgccaaaaatgctgatg
ctgccaaatggaactcggtgttcgatggacgatacatcattgttcgatcattcgatttca
atagacaaagctatgcccaagacacaactgaaacttttgtaactgatccaaataaccaga
tgtactttaagttcaatgtgaaatatttggatactaatggaccaactacattggatgtta
acgtctacaaaaatggagttgctgtcttcacagcaacatatactgccacaaatcccccat
tgcctttcccgaacggtatcttggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAAAACGTGTCTGCGGTGGCCAC 241 73 806 Antisense
GGTGGCCACTATTTCGAGACAACCT 70 499 822 Antisense
CAACCTGATTACTTGACTCTTCCGA 456 187 841 Antisense
AACCTTTTTGCCACAATCAACTCCA 91 143 922 Antisense
CTACCAGTCAATGTTCCCACTAAGA 268 225 948 Antisense
GGATTACTTACGGTTCCAGTTGCCA 287 505 1009 Antisense
GGACGATACATCATTGTTCGATCAT 580 523 1072 Antisense
GACAAAGCTATGCCCAAGACACAAC 109 365 1109 Antisense
AAAAATGGAGTTGCTGTCTTCACAG 18 131 1234 Antisense
GCAACATATACTGCCACAAATCCCC 664 317 1258 Antisense
TGCCTTTCCCGAACGGTATCTTGGG 505 583 1286 Antisense
Sequence Source Affymetrix Proprietary Database