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[179634_at]

Description F53H2.3 /REP_DB=WormBase Gene ID /WP=CE19888 /TR=Q9U3E4 /GB=CAB63205.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 28      4grp_total > 12      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 35.8
(3)
A 		12.9
(-3)
A 		17.7
(-1.7)
A 		30.9
(1.7)
A 		23
B 6.9
(-3)
A 		10.1
(-2.2)
A 		15.3
(-0.8)
A 		30.3
(3)
A 		23
C 12
(-0.2)
A 		18.7
(2.2)
A 		3.9
(-3)
A 		21.1
(3)
A 		17
avr 18.2
(-0.7) 		13.9
(-2.4) 		12.3
(-3) 		27.4
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 32.1
(3)
A 		13.5
(-0.9)
A 		7
(-2.2)
A 		3.2
(-3)
A 		8.7
(-1.9)
A 		9.8
(-1.6)
A 		29
B 37.3
(3)
A 		5.5
(-2.6)
A 		9.9
(-1.8)
A 		3.1
(-3)
A 		10.2
(-1.8)
A 		4.9
(-2.7)
A 		34
C 17.3
(3)
A 		11.2
(0.3)
A 		4.8
(-2.6)
A 		3.9
(-3)
A 		10.5
(-0)
A 		14.5
(1.7)
A 		13
avr 28.9
(3) 		10.1
(-1.4) 		7.2
(-2.1) 		3.4
(-3) 		9.8
(-1.5) 		9.7
(-1.5) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 13
(3)
A 		2.8
(-3)
A 		4.6
(-1.9)
A 		4.5
(-2)
A 		10
B 2.2
(-3)
A 		2.5
(-2.6)
A 		3.5
(-1.2)
A 		6.5
(3)
A 		4
C 4
(0.9)
A 		5.4
(3)
A 		1.4
(-3)
A 		3.6
(0.3)
A 		4
avr 6.4
(3) 		3.6
(-2.3) 		3.2
(-3) 		4.9
(0.2) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 5.9
(3)
A 		4.8
(1.7)
A 		1.5
(-2.2)
A 		0.8
(-3)
A 		2.6
(-0.9)
A 		2.4
(-1.1)
A 		5
B 13
(3)
A 		1.8
(-2.5)
A 		2.4
(-2.2)
A 		0.8
(-3)
A 		4.1
(-1.4)
A 		1.8
(-2.5)
A 		12
C 6.4
(3)
A 		3.4
(-0.2)
A 		1.2
(-2.6)
A 		0.8
(-3)
A 		3.4
(-0.2)
A 		4.8
(1.3)
A 		6
avr 8.4
(3) 		3.3
(-1) 		1.7
(-2.3) 		0.8
(-3) 		3.4
(-0.9) 		3
(-1.3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179634_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12542
Sequence Type Exemplar sequence
Representative Public ID F53H2.3 NCBI
Target Description F53H2.3 /REP_DB=WormBase Gene ID /WP=CE19888 /TR=Q9U3E4 /GB=CAB63205.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075530(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075530 NCBI Caenorhabditis elegans F53H2.3 (F53H2.3) mRNA, complete cds. 11/11 None
SNAP00000018992 ENSEMBL cdna:SNAP chromosome:CEL140:V:20392578:20406347:-1 11/11 None
GENEFINDER00000018994 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20395159:20408532:-1 11/11 None
F53H2.3 ENSEMBL cdna:known chromosome:CEL140:V:20394903:20408532:-1 gene:F53H2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20395158-20408532(-) 95.26 100.0
Public Domain and Genome References
Gene Title
Ensembl F53H2.3
Entrez Gene 180338 Entrez gene
SwissProt Q9U3E4 EMBL-EBI
WormBase CE19888 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB63205 Hypothetical protein F53H2.3 [Caenorhabditis elegans] ref|NP_507931.1| F53H2.3 [Caenorhabditis elegans] 0.0
blast CAE70988 Hypothetical protein CBG17813 [Caenorhabditis briggsae] 1.0E-52
blast CAE70988 Hypothetical protein CBG17813 [Caenorhabditis briggsae] 8.0E-46
blast CAE70988 Hypothetical protein CBG17813 [Caenorhabditis briggsae] 9.0E-46
blast AAT12421 hypothetical protein isoform b [Dictyocaulus viviparus] 2.0E-25
blast AAT12422 hypothetical protein isoform a [Dictyocaulus viviparus] 8.0E-22
Sequence
Target Sequence 
>C. ELEGANS:179634_AT
gcagccacgtggtgattctacgtcatcaagtcagtgcggggacacatttgccgagctctt
cagctcatcggatcccgtctacattccacgcccgagtgctctgaatttggagcaaaaagc
tgtgtcgccgtcggaaaactcgacggcagcactgtttgacgcggttttggccgattttgg
aggctttaaggcaacatcagcaaatctcaatccacttctaccaggaatatcatcatcgca
aagtccgatgaaaggagaatcatggaattcgttccgaagttgctcacttttcagtccaat
taagccgtcgaaacgggctcctctgagctccaacggtctacagacacaggcttctgctgc
tgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGCCACGTGGTGATTCTACGTCA 707 313 1464 Antisense
TCTACGTCATCAAGTCAGTGCGGGG 341 611 1480 Antisense
AGTGCGGGGACACATTTGCCGAGCT 414 59 1496 Antisense
AGCTGTGTCGCCGTCGGAAAACTCG 329 81 1581 Antisense
ACTCGACGGCAGCACTGTTTGACGC 109 99 1601 Antisense
GACGCGGTTTTGGCCGATTTTGGAG 674 375 1621 Antisense
AAATCTCAATCCACTTCTACCAGGA 509 115 1665 Antisense
AAGTTGCTCACTTTTCAGTCCAATT 49 157 1740 Antisense
TTAAGCCGTCGAAACGGGCTCCTCT 480 657 1763 Antisense
TGAGCTCCAACGGTCTACAGACACA 149 569 1787 Antisense
TACAGACACAGGCTTCTGCTGCTGC 197 643 1802 Antisense
Sequence Source Affymetrix Proprietary Database