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[179632_s_at]

Description F45D3.3 /REP_DB=WormBase Gene ID /WP=CE10472 /TR=Q20421 /GB=CAB01502.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 09      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 13      6grp_total > 04      4grp_total > 46      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2714
1 2 3 4 range
A 4476.6
(3)
P 		2729.3
(-1.3)
P 		2020.8
(-3)
P 		2413.9
(-2)
P 		2456
B 4009.9
(3)
P 		2346.3
(-1.4)
P 		1762.8
(-3)
P 		2189.6
(-1.9)
P 		2247
C 4361.9
(3)
P 		2732.2
(-1)
P 		1904.5
(-3)
P 		2162.6
(-2.4)
P 		2457
avr 4282.8
(3) 		2602.6
(-1.2) 		1896
(-3) 		2255.4
(-2.1) 		2387

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1673
1 2 3 4 5 6 range
A 3029.7
(2.3)
P 		3189.2
(3)
P 		2265.8
(-1.1)
P 		3048.2
(2.4)
P 		3031.3
(2.3)
P 		1826.9
(-3)
P 		1362
B 3316.4
(3)
P 		3181.8
(2.5)
P 		1976.6
(-2.2)
P 		2953.8
(1.6)
P 		3176.6
(2.5)
P 		1761.1
(-3)
P 		1555
C 3342.1
(3)
P 		2948.2
(1.6)
P 		2817.5
(1.1)
P 		2365.8
(-0.5)
P 		3109
(2.2)
P 		1669.3
(-3)
P 		1673
avr 3229.4
(3) 		3106.4
(2.5) 		2353.3
(-0.6) 		2789.3
(1.2) 		3105.6
(2.5) 		1752.4
(-3) 		1477
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1277
1 2 3 4 range
A 1629.9
(3)
P 		592.5
(-1.9)
P 		528.3
(-2.2)
P 		353.4
(-3)
P 		1277
B 1278.7
(3)
P 		579.5
(-1.8)
P 		408.5
(-3)
P 		466.6
(-2.6)
P 		870
C 1443.9
(3)
P 		785.5
(-0.7)
P 		663.8
(-1.3)
P 		364.6
(-3)
P 		1079
avr 1450.8
(3) 		652.5
(-1.5) 		533.5
(-2.2) 		394.9
(-3) 		1056

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 846
1 2 3 4 5 6 range
A 558.5
(-2)
P 		1134.2
(3)
P 		493.4
(-2.6)
P 		773.1
(-0.1)
P 		914.7
(1.1)
P 		444.8
(-3)
P 		689
B 1153.9
(2)
P 		1059.1
(1.3)
P 		480
(-3)
P 		782.4
(-0.8)
P 		1290.6
(3)
P 		656.7
(-1.7)
P 		811
C 1238.8
(3)
P 		899.6
(0.3)
P 		717.1
(-1.1)
P 		475.8
(-3)
P 		1006
(1.2)
P 		558.8
(-2.3)
P 		763
avr 983.7
(2) 		1031
(2.5) 		563.5
(-2.9) 		677.1
(-1.6) 		1070.4
(3) 		553.4
(-3) 		517
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179632_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13689
Sequence Type Exemplar sequence
Representative Public ID F45D3.3 NCBI
Target Description F45D3.3 /REP_DB=WormBase Gene ID /WP=CE10472 /TR=Q20421 /GB=CAB01502.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073741(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073741 NCBI Caenorhabditis elegans F45D3.3 (F45D3.3) mRNA, complete cds. 11/11 None
SNAP00000028860 ENSEMBL cdna:SNAP chromosome:CEL140:V:12552050:12552877:-1 11/11 None
GENEFINDER00000028864 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12552050:12552877:-1 11/11 None
F45D3.3 ENSEMBL cdna:known chromosome:CEL140:V:12551802:12552940:-1 gene:F45D3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12552049-12552877(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45D3.3
Entrez Gene 179717 Entrez gene
SwissProt Q20421 EMBL-EBI
WormBase CE10472 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01502 Hypothetical protein F45D3.3 [Caenorhabditis elegans] ref|NP_506142.1| F45D3.3 [Caenorhabditis elegans] 1.0E-119
blast CAE75510 Hypothetical protein CBG23524 [Caenorhabditis briggsae] 1.0E-104
blast CAB01501 Hypothetical protein F45D3.4a [Caenorhabditis elegans] ref|NP_001023930.1| F45D3.4a [Caenorhabditis elegans] 2.0E-73
Sequence
Target Sequence 
>C. ELEGANS:179632_S_AT
gatcttgacctgttcttgggttcaatgggagatggacactttgctaatgtcgatatggat
ggagtagaaacaatggttccaaagaaatctgaaaagcttccaggacaaaagaaaatgaag
ccggaattccaacaaccagaaggtgttgccccagaattcactggtggatttatgggctcg
gatgaagcagttttagccgctgagccatcgacacaaaaaactgatgaaattgttgaatct
gccgagtctgattcctcatctgataccgactacttcggtgaagcttctgcgtctgctcca
gtttccaacccagctctcacctacctttctggagctaactttgacaatgttaaacaacca
tcctgccaaatgctcggatgcactggaccaattccaaacgacggatcatatgctgccatg
tcagcttctcttgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTTGACCTGTTCTTGGGTTCAA 572 419 115 Antisense
AGAAGGTGTTGCCCCAGAATTCACT 423 75 252 Antisense
TCACTGGTGGATTTATGGGCTCGGA 392 623 272 Antisense
TGAAGCAGTTTTAGCCGCTGAGCCA 82 575 297 Antisense
TGAATCTGCCGAGTCTGATTCCTCA 463 575 348 Antisense
TGATTCCTCATCTGATACCGACTAC 42 565 363 Antisense
ACTACTTCGGTGAAGCTTCTGCGTC 369 101 383 Antisense
TCACCTACCTTTCTGGAGCTAACTT 80 381 431 Antisense
CAATGTTAAACAACCATCCTGCCAA 526 181 459 Antisense
AACGACGGATCATATGCTGCCATGT 429 145 511 Antisense
TGCTGCCATGTCAGCTTCTCTTGAA 607 581 525 Antisense
Sequence Source Affymetrix Proprietary Database