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[179613_at]

Description C50F4.8 /REP_DB=WormBase Gene ID /WP=CE05472 /TR=Q18743 /GB=CAA94743.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 62      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 35      6grp_total > 62      4grp_total > 52      6grp_highest_sub > 04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2676
1 2 3 4 range
A 3030.6
(2.7)
P 		3134
(3)
P 		2663.7
(1.7)
P 		905
(-3)
P 		2229
B 3091.5
(2.9)
P 		3117.9
(3)
P 		2853.6
(2.3)
P 		789.5
(-3)
P 		2328
C 3429.3
(3)
P 		2987.8
(2)
P 		2554
(1)
P 		753.4
(-3)
P 		2676
avr 3183.8
(3) 		3079.9
(2.7) 		2690.4
(1.7) 		816
(-3) 		2368

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4068
1 2 3 4 5 6 range
A 1942.9
(-2.3)
P 		3494.8
(0.3)
P 		5029.4
(3)
P 		4544.6
(2.2)
P 		4117.8
(1.4)
P 		1562.8
(-3)
P 		3467
B 2077.9
(-2)
P 		2923.4
(-0.7)
P 		5464.7
(3)
P 		4181.6
(1.1)
P 		3494.2
(0.1)
P 		1396.4
(-3)
P 		4068
C 2042
(-2.1)
P 		3086.8
(-0.4)
P 		5200.4
(3)
P 		4638.2
(2.1)
P 		4255.7
(1.5)
P 		1490.7
(-3)
P 		3710
avr 2020.9
(-2.1) 		3168.3
(-0.3) 		5231.5
(3) 		4454.8
(1.8) 		3955.9
(1) 		1483.3
(-3) 		3748
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1008
1 2 3 4 range
A 1103.4
(3)
P 		680.3
(0.4)
P 		696.3
(0.5)
P 		132.5
(-3)
P 		971
B 985.8
(3)
P 		770
(1.4)
P 		661.2
(0.6)
P 		168.3
(-3)
P 		818
C 1135.2
(3)
P 		859
(1.4)
P 		890.1
(1.5)
P 		127
(-3)
P 		1008
avr 1074.8
(3) 		769.8
(1) 		749.2
(0.9) 		142.6
(-3) 		932

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1062
1 2 3 4 5 6 range
A 358.2
(-3)
P 		1242.9
(3)
P 		1095.3
(2)
P 		1152.6
(2.4)
P 		1242.6
(3)
P 		380.5
(-2.8)
P 		885
B 723
(-1.6)
P 		973.1
(0)
P 		1327
(2.4)
P 		1107.5
(0.9)
P 		1419.7
(3)
P 		520.7
(-3)
P 		899
C 756.9
(-1.2)
P 		941.9
(0)
P 		1323.6
(2.6)
P 		932.8
(-0)
P 		1377.1
(3)
P 		499
(-3)
P 		878
avr 612.7
(-2) 		1052.6
(1) 		1248.6
(2.3) 		1064.3
(1.1) 		1346.5
(3) 		466.7
(-3) 		880
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179613_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12299
Sequence Type Exemplar sequence
Representative Public ID C50F4.8 NCBI
Target Description C50F4.8 /REP_DB=WormBase Gene ID /WP=CE05472 /TR=Q18743 /GB=CAA94743.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073052(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073052 NCBI Caenorhabditis elegans C50F4.8 (C50F4.8) mRNA, complete cds. 11/11 None
SNAP00000009306 ENSEMBL cdna:SNAP chromosome:CEL140:V:9520237:9521433:-1 11/11 None
GENEFINDER00000009322 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9520237:9521433:-1 11/11 None
C50F4.8.1 ENSEMBL cdna:known chromosome:CEL140:V:9519898:9521459:-1 gene:C50F4.8 11/11 None
C50F4.8.2 ENSEMBL cdna:known chromosome:CEL140:V:9520105:9521433:-1 gene:C50F4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9520236-9521433(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50F4.8
Entrez Gene 179332 Entrez gene
SwissProt Q18743 EMBL-EBI
WormBase CE05472 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94743 Hypothetical protein C50F4.8 [Caenorhabditis elegans] ref|NP_505453.1| C50F4.8 [Caenorhabditis elegans] 1.0E-165
blast CAE72187 Hypothetical protein CBG19295 [Caenorhabditis briggsae] 1.0E-148
Sequence
Target Sequence 
>C. ELEGANS:179613_AT
tcaaaatgcactcctggattcctccatcaacttctcatgcgaagctctgaaattatcgat
caatattgtgtctataatttcgatgcgattcgtgagaagttcccatgtcttatcaaaatg
aagccaaataaggaatgcgtcaaaacatgtactccacatcacaatgccatctcatccatg
actgaaaatttcaagaatcttgtgctcagcggggatactacaaaggctgaacaatatttg
gctcaaggatgtgaatatatcacttgtactcttcattgtgatgttccaacaattgcacat
gaatgtgattcggagactgctcaacttgtgattgacctaactcgtaagtcatttgcaagc
atggaaaaaatggcacttgatacgaatgctgttgctaaatggccacaagtttgtgctgat
atcaagacatacagaatgccaaccccatcaattccaccaaagaacactgatgtcgtagcg
caaagcaattcagtcgctgatcaaccaatttcacatccaaaattggtacaagctccactt
atcagagcttcatcgattctgtcgatctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAAATGCACTCCTGGATTCCTCC 10 631 310 Antisense
GATTCGTGAGAAGTTCCCATGTCTT 341 433 396 Antisense
CATGTACTCCACATCACAATGCCAT 182 209 455 Antisense
CAATGCCATCTCATCCATGACTGAA 267 183 471 Antisense
GAATCTTGTGCTCAGCGGGGATACT 26 327 504 Antisense
ATATATCACTTGTACTCTTCATTGT 709 19 564 Antisense
GTGATTCGGAGACTGCTCAACTTGT 670 483 614 Antisense
AAGACATACAGAATGCCAACCCCAT 104 153 733 Antisense
GTCGCTGATCAACCAATTTCACATC 210 475 802 Antisense
ATTGGTACAAGCTCCACTTATCAGA 533 3 831 Antisense
GAGCTTCATCGATTCTGTCGATCTC 175 389 854 Antisense
Sequence Source Affymetrix Proprietary Database