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[179589_at]

Description W04A4.3 /REP_DB=WormBase Gene ID /WP=CE14572 /TR=O45889 /GB=CAB04897.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 35      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 32.8
(-1.8)
A 		88.9
(3)
A 		19.3
(-3)
A 		66.2
(1)
A 		70
B 36.9
(-0.9)
A 		92.9
(3)
A 		6.7
(-3)
A 		23.6
(-1.8)
A 		86
C 5.6
(-3)
A 		28.4
(-0.1)
A 		46.4
(2.2)
A 		52.8
(3)
A 		47
avr 25.1
(-2.9) 		70.1
(3) 		24.1
(-3) 		47.5
(0.1) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 67.3
(3)
A 		5.9
(-3)
A 		9.7
(-2.6)
A 		33.7
(-0.3)
A 		38.5
(0.2)
A 		5.6
(-3)
A 		62
B 5.3
(-3)
A 		10.8
(-2.3)
A 		32.7
(0.7)
A 		18.3
(-1.3)
A 		16.6
(-1.5)
A 		49.9
(3)
A 		45
C 4.4
(-2.6)
A 		2.3
(-3)
A 		29.7
(2.8)
A 		26.7
(2.1)
A 		30.8
(3)
A 		4.9
(-2.5)
A 		29
avr 25.7
(2.2) 		6.3
(-3) 		24
(1.8) 		26.2
(2.4) 		28.6
(3) 		20.1
(0.7) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 11.9
(-0.1)
A 		19.3
(3)
A 		5
(-3)
A 		9.7
(-1)
A 		14
B 11.8
(-0.1)
A 		22.9
(3)
A 		1.6
(-3)
A 		5
(-2)
A 		21
C 1.8
(-3)
A 		8.2
(-0.3)
A 		16.2
(3)
A 		8.9
(-0)
A 		14
avr 8.5
(-2.4) 		16.8
(3) 		7.6
(-3) 		7.9
(-2.8) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 12.4
(3)
A 		2.1
(-2.6)
A 		2.1
(-2.6)
A 		8.5
(0.9)
A 		11.6
(2.6)
A 		1.4
(-3)
A 		11
B 1.8
(-3)
A 		3.6
(-2.4)
A 		7.9
(-0.8)
A 		4.8
(-1.9)
A 		6.7
(-1.3)
A 		18.6
(3)
A 		17
C 1.6
(-2.4)
A 		0.7
(-3)
A 		7.6
(1.5)
A 		5.4
(0)
A 		10
(3)
A 		1.6
(-2.4)
A 		9
avr 5.3
(-0.4) 		2.1
(-3) 		5.9
(0.1) 		6.2
(0.4) 		9.4
(3) 		7.2
(1.2) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179589_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.551
Sequence Type Exemplar sequence
Representative Public ID W04A4.3 NCBI
Target Description W04A4.3 /REP_DB=WormBase Gene ID /WP=CE14572 /TR=O45889 /GB=CAB04897.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060997(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060997 NCBI Caenorhabditis elegans W04A4.3 (W04A4.3) mRNA, complete cds. 11/11 None
SNAP00000016127 ENSEMBL cdna:SNAP chromosome:CEL140:I:13688533:13689484:-1 11/11 None
W04A4.3 ENSEMBL cdna:known chromosome:CEL140:I:13688533:13689484:-1 gene:W04A4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13688463-13689415(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W04A4.3
Entrez Gene 189179 Entrez gene
SwissProt O45889 EMBL-EBI
WormBase CE14572 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04897 Hypothetical protein W04A4.3 [Caenorhabditis elegans] ref|NP_493398.1| W04A4.3 [Caenorhabditis elegans] 1.0E-83
blast CAE63501 Hypothetical protein CBG07974 [Caenorhabditis briggsae] 7.0E-12
blast CAA22309 Hypothetical protein Y18D10A.2 [Caenorhabditis elegans] ref|NP_493240.1| Y18D10A.2 [Caenorhabditis elegans] 1.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR004911 EMBL-EBI Gamma interferon inducible lysosomal thiol reductase GILT 4.1E-48
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493398.1 1 7-29
W04A4.3 1 7-29
SNAP00000016127 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:179589_AT
acccgccaaacagtcaattttcgtgacaatggttatgatctactccgtcatcctaattat
aacaatattcacacctgttcagcaaatccatgtagatattattgatatcactggattcgc
aaagtgtgctcttacaacaaaatggttcagaactcagctggcgccgtttttggggaattt
aacggcgaagaagggtccgaagaacttgaagatggtctatcatcctttgtcgattggaca
gaaaaagggtaatggaacagctgtggccacgtgtgaaaatggatggttggagtgtcaact
gaacaagcttcagtgttgtacaaaaaaatactcgagaaatgttacggatttcgagattct
cgcggttttggagtgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCCGCCAAACAGTCAATTTTCGTG 23 91 18 Antisense
ATGGTTATGATCTACTCCGTCATCC 463 51 46 Antisense
TCTACTCCGTCATCCTAATTATAAC 157 615 56 Antisense
TATTCACACCTGTTCAGCAAATCCA 566 663 83 Antisense
GATTCGCAAAGTGTGCTCTTACAAC 162 433 131 Antisense
AATGGTTCAGAACTCAGCTGGCGCC 422 163 158 Antisense
TGGTCTATCATCCTTTGTCGATTGG 688 551 230 Antisense
GGAACAGCTGTGGCCACGTGTGAAA 221 529 271 Antisense
TGGAGTGTCAACTGAACAAGCTTCA 616 549 305 Antisense
ATGTTACGGATTTCGAGATTCTCGC 637 47 356 Antisense
GAGATTCTCGCGGTTTTGGAGTGTA 68 397 370 Antisense
Sequence Source Affymetrix Proprietary Database