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[179579_at]

Description F53F4.2 /REP_DB=WormBase Gene ID /WP=CE10956 /TR=Q20725 /GB=CAB01209.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 42      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 162
1 2 3 4 range
A 137
(-3)
A 		187.6
(1.4)
P 		206.3
(3)
P 		153.6
(-1.6)
A 		69
B 160.7
(-3)
A 		263.1
(3)
P 		190.4
(-1.3)
P 		161.7
(-2.9)
A 		102
C 156.6
(-3)
M 		299.4
(3)
P 		180.5
(-2)
M 		217.8
(-0.4)
A 		143
avr 151.4
(-3) 		250
(3) 		192.4
(-0.5) 		177.7
(-1.4) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 306.9
(3)
P 		167
(-0.6)
M 		200.8
(0.2)
M 		110.9
(-2.1)
A 		75.8
(-3)
A 		80.8
(-2.9)
A 		231
B 215.4
(3)
P 		199.2
(2.3)
M 		162
(0.6)
A 		112.1
(-1.6)
A 		79.7
(-3)
M 		83.5
(-2.8)
A 		136
C 171.7
(0.3)
M 		237.1
(3)
M 		138.2
(-1.1)
A 		106.8
(-2.4)
A 		97.1
(-2.8)
A 		92.2
(-3)
A 		145
avr 231.3
(3) 		201.1
(1.8) 		167
(0.4) 		109.9
(-2) 		84.2
(-3) 		85.5
(-2.9) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 49.9
(2.2)
A 		40.7
(0.5)
P 		53.9
(3)
P 		22.5
(-3)
A 		31
B 51.3
(0.3)
A 		65
(3)
P 		44.1
(-1.1)
P 		34.5
(-3)
A 		31
C 51.8
(-1.2)
M 		86.1
(3)
P 		62.9
(0.2)
M 		36.7
(-3)
A 		49
avr 51
(0.6) 		63.9
(3) 		53.6
(1.1) 		31.2
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 56.6
(2.6)
P 		59.4
(3)
M 		43.7
(0.6)
M 		28.1
(-1.7)
A 		22.9
(-2.5)
A 		19.7
(-3)
A 		40
B 74.9
(3)
P 		66.3
(1.9)
M 		39.3
(-1.7)
A 		29.7
(-3)
A 		32.4
(-2.6)
M 		31.1
(-2.8)
A 		45
C 63.6
(2)
M 		72.3
(3)
M 		35.2
(-1.4)
A 		21.5
(-3)
A 		31.4
(-1.8)
A 		30.8
(-1.9)
A 		51
avr 65
(2.8) 		66
(3) 		39.4
(-1) 		26.4
(-3) 		28.9
(-2.6) 		27.2
(-2.9) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179579_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13434
Sequence Type Exemplar sequence
Representative Public ID F53F4.2 NCBI
Target Description F53F4.2 /REP_DB=WormBase Gene ID /WP=CE10956 /TR=Q20725 /GB=CAB01209.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073965(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073965 NCBI Caenorhabditis elegans F53F4.2 (F53F4.2) mRNA, complete cds. 11/11 None
GENEFINDER00000012459 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13591204:13592477:-1 11/11 None
F53F4.2 ENSEMBL cdna:known chromosome:CEL140:V:13591204:13592477:-1 gene:F53F4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012433 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13591203-13592477(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F4.2
Entrez Gene 186175 Entrez gene
SwissProt Q20725 EMBL-EBI
WormBase CE10956 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01209 Hypothetical protein F53F4.2 [Caenorhabditis elegans] ref|NP_506366.1| F53F4.2 [Caenorhabditis elegans] 2.0E-79
blast CAE66285 Hypothetical protein CBG11532 [Caenorhabditis briggsae] 1.0E-24
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506366.1 3 79-101,122-144,159-181
F53F4.2 3 79-101,122-144,159-181
GENEFINDER00000012459 3 79-101,122-144,159-181
Sequence
Target Sequence 
>C. ELEGANS:179579_AT
gaatcccaccaaatcttgaatccatggtgcctcaaaagaaatatgaaaagagcagaacaa
agcacttataaaacattgaaatttcaaaaaccaacttcatttttgaaccacttgaaatat
acaagtggacgcctgaaaaagggaaaaagaaggtcacctctttacgactgtcttctcatt
tttcatcattcttccctctttttaccggtcatgatttgtcacctccttattctcctattc
cccgttatttccatatctcaatattataacccatccctataccaaagctatatgaatgcc
ggtgttccccggcaacagactgtgttggttcccgtctacacacaaggtggctatggtgga
gcatatggtggttatccgagttatgggcaatcttatggacagagttatccatcttatgga
atgggaatgggctatggatatgctgcaggataccccatgatgggcggagctggaggagca
tgttgtggaggaacaggaatgggaatgatgccacctcgtgaaagcaacgcgaaaaagttt
ctccgcggagccacagaagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCCCACCAAATCTTGAATCCAT 24 331 58 Antisense
AAGAAGGTCACCTCTTTACGACTGT 108 153 204 Antisense
TTACGACTGTCTTCTCATTTTTCAT 676 681 219 Antisense
CCCTCTTTTTACCGGTCATGATTTG 691 257 251 Antisense
TCCCCGTTATTTCCATATCTCAATA 634 595 296 Antisense
TATTATAACCCATCCCTATACCAAA 598 659 319 Antisense
TGAATGCCGGTGTTCCCCGGCAACA 392 573 350 Antisense
TTCCCGTCTACACACAAGGTGGCTA 314 695 386 Antisense
GATATGCTGCAGGATACCCCATGAT 17 427 494 Antisense
TGATGCCACCTCGTGAAAGCAACGC 702 565 563 Antisense
GTTTCTCCGCGGAGCCACAGAAGGA 203 451 594 Antisense
Sequence Source Affymetrix Proprietary Database