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[179571_at]

Description C27D8.4 /REP_DB=WormBase Gene ID /WP=CE08423 /TR=Q93291 /GB=CAB02264.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 82      4grp_high_8_8 > 05      6grp_total > 73      4grp_total > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 407
1 2 3 4 range
A 248.4
(-1.9)
P 		210.1
(-2.8)
P 		199.3
(-3)
P 		478.5
(3)
P 		279
B 293.6
(-0.9)
P 		233.1
(-2)
P 		178.8
(-3)
P 		508.4
(3)
P 		330
C 261.6
(-1.5)
P 		157.8
(-3)
A 		198.8
(-2.4)
P 		564.7
(3)
P 		407
avr 267.9
(-1.6) 		200.3
(-2.9) 		192.3
(-3) 		517.2
(3) 		325

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 295
1 2 3 4 5 6 range
A 316.1
(-3)
A 		338.6
(-2.1)
P 		355.6
(-1.4)
P 		462.3
(3)
P 		374
(-0.6)
P 		402.8
(0.6)
P 		146
B 192.1
(-3)
P 		329.5
(0.1)
P 		460
(3)
P 		371.4
(1)
P 		428
(2.3)
P 		307.5
(-0.4)
P 		268
C 227.3
(-3)
P 		336.3
(-0.5)
P 		347.5
(-0.2)
P 		486.8
(3)
P 		442.2
(2)
P 		393.2
(0.8)
P 		260
avr 245.2
(-3) 		334.8
(-0.2) 		387.7
(1.4) 		440.2
(3) 		414.7
(2.2) 		367.8
(0.8) 		195
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 90.5
(3)
P 		45.6
(-3)
P 		52.1
(-2.1)
P 		70
(0.3)
P 		45
B 93.6
(1.7)
P 		57.6
(-1.5)
P 		41.4
(-3)
P 		108.3
(3)
P 		67
C 86.6
(2)
P 		45.4
(-3)
A 		69.3
(-0.1)
P 		95.2
(3)
P 		50
avr 90.2
(2.9) 		49.5
(-3) 		54.3
(-2.3) 		91.2
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 58.3
(-3)
A 		120.4
(3)
P 		77.4
(-1.2)
P 		117.2
(2.7)
P 		112.9
(2.3)
P 		98.1
(0.8)
P 		62
B 66.8
(-3)
P 		109.7
(-0.6)
P 		111.7
(-0.5)
P 		98.4
(-1.2)
P 		173.9
(3)
P 		114.7
(-0.3)
P 		107
C 84.3
(-3)
P 		102.6
(-1.1)
P 		88.4
(-2.6)
P 		97.9
(-1.6)
P 		143.1
(3)
P 		131.6
(1.8)
P 		59
avr 69.8
(-3) 		110.9
(0.4) 		92.5
(-1.1) 		104.5
(-0.2) 		143.3
(3) 		114.8
(0.7) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179571_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10361
Sequence Type Exemplar sequence
Representative Public ID C27D8.4 NCBI
Target Description C27D8.4 /REP_DB=WormBase Gene ID /WP=CE08423 /TR=Q93291 /GB=CAB02264.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070024(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070024 NCBI Caenorhabditis elegans C27D8.4 (C27D8.4) mRNA, complete cds. 11/11 None
SNAP00000003310 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12704097:12706680:1 11/11 None
GENEFINDER00000003316 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12704097:12706680:1 11/11 None
C27D8.4 ENSEMBL cdna:known chromosome:CEL140:IV:12704097:12706680:1 gene:C27D8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12704102-12706686(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27D8.4
Entrez Gene 178223 Entrez gene
SwissProt Q93291 EMBL-EBI
WormBase CE30618 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02264 Hypothetical protein C27D8.4 [Caenorhabditis elegans] ref|NP_502425.2| C27D8.4 [Caenorhabditis elegans] 0.0
blast CAB02264 Hypothetical protein C27D8.4 [Caenorhabditis elegans] ref|NP_502425.2| C27D8.4 [Caenorhabditis elegans] 1.0E-153
blast CAE56981 Hypothetical protein CBG24834 [Caenorhabditis briggsae] 1.0E-100
blast CAE56981 Hypothetical protein CBG24834 [Caenorhabditis briggsae] 4.0E-91
blast CAE59995 Hypothetical protein CBG03488 [Caenorhabditis briggsae] 1.0E-67
blast CAE59995 Hypothetical protein CBG03488 [Caenorhabditis briggsae] 2.0E-67
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502425.2 1 21-43
C27D8.4 1 21-43
SNAP00000003310 1 21-43
GENEFINDER00000003316 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:179571_AT
ttggaaagcggattctccttctcgccaatcaactcaaatcccatcaattcgacgtggcaa
tattcgctgcaggaatcatgttagcaccggaatcccgaacgaaagatggcgtcgaacttc
acaatgcagttaacgtaatcggacaagtaatgctctacgagcttctacaaaatgaatgca
aacgagcaatatttctatcgtctgccacagcgagaatggcttgttactctttggattcca
attttttgaaagtttacgcaggaccatatcaagcatatgcatcttcaaagctgaatctag
ctgtttatgtgaatgaagttgccaagaaaagacgcgtcaatacagtaagtcttcatcctg
gcaccgtgccaggccgtctctatcaaaatgcaaatccaattgttcgatatctgaatgcca
cactgcttccaaaactgatgagaaccccagaaatggcggctgttctcgtgcttcacacga
ttttccgagacgacatccaacctggagcctattatgaagatactcaaatagtgaatgtac
cagaatgggtcccggcacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGAAAGCGGATTCTCCTTCTCGC 81 709 281 Antisense
ACTCAAATCCCATCAATTCGACGTG 350 101 312 Antisense
GAATCATGTTAGCACCGGAATCCCG 536 325 353 Antisense
GATGGCGTCGAACTTCACAATGCAG 47 407 385 Antisense
TTTCTATCGTCTGCCACAGCGAGAA 110 673 472 Antisense
AATGGCTTGTTACTCTTTGGATTCC 148 163 495 Antisense
TACAGTAAGTCTTCATCCTGGCACC 710 643 621 Antisense
CGATATCTGAATGCCACACTGCTTC 326 249 685 Antisense
GTGCTTCACACGATTTTCCGAGACG 560 477 748 Antisense
TTCCGAGACGACATCCAACCTGGAG 481 695 763 Antisense
TGTACCAGAATGGGTCCCGGCACGA 31 557 816 Antisense
Sequence Source Affymetrix Proprietary Database