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[179564_at]

Description T22B3.3 /REP_DB=WormBase Gene ID /WP=CE03695 /TR=Q22660 /GB=CAA92617.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 36.1
(-1.5)
A 		18.1
(-3)
A 		20.4
(-2.8)
A 		88.7
(3)
A 		71
B 18.3
(-1.4)
A 		10
(-2.2)
A 		66
(3)
A 		1.6
(-3)
A 		64
C 4
(-3)
A 		80.9
(3)
A 		19.5
(-1.8)
A 		54.3
(0.9)
A 		77
avr 19.5
(-3) 		36.3
(0.5) 		35.3
(0.3) 		48.2
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 334
1 2 3 4 5 6 range
A 37.1
(-3)
A 		36.8
(-3)
A 		49.2
(-2.7)
A 		51.9
(-2.7)
P 		225
(1.3)
P 		301.8
(3)
P 		265
B 9.1
(-3)
A 		35.6
(-2.4)
A 		70.3
(-1.7)
A 		38
(-2.4)
A 		180.3
(0.6)
P 		297.4
(3)
P 		288
C 53.2
(-2.6)
A 		32.1
(-3)
A 		40.6
(-2.8)
A 		63.6
(-2.4)
A 		161.3
(-0.5)
P 		343
(3)
P 		311
avr 33.1
(-3) 		34.8
(-3) 		53.4
(-2.6) 		51.2
(-2.6) 		188.9
(0.3) 		314.1
(3) 		281
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 13.1
(3)
A 		3.9
(-3)
A 		5.3
(-2.1)
A 		13
(2.9)
A 		9
B 5.8
(-0.8)
A 		2.5
(-2.1)
A 		15.3
(3)
A 		0.3
(-3)
A 		15
C 1.3
(-3)
A 		23.2
(3)
A 		6.8
(-1.5)
A 		9.2
(-0.8)
A 		22
avr 6.7
(-3) 		9.9
(3) 		9.1
(1.5) 		7.5
(-1.5) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 6.8
(-3)
A 		13.1
(-2.4)
A 		10.7
(-2.6)
A 		13.2
(-2.4)
P 		67.9
(2.5)
P 		73.5
(3)
P 		67
B 3.2
(-3)
A 		11.9
(-2.5)
A 		17.1
(-2.2)
A 		10.1
(-2.6)
A 		73.2
(0.9)
P 		110.9
(3)
P 		108
C 19.7
(-2.4)
A 		9.8
(-3)
A 		10.3
(-3)
A 		12.8
(-2.8)
A 		52.2
(-0.6)
P 		114.8
(3)
P 		105
avr 9.9
(-3) 		11.6
(-2.9) 		12.7
(-2.8) 		12
(-2.9) 		64.4
(0.6) 		99.7
(3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179564_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8807
Sequence Type Exemplar sequence
Representative Public ID T22B3.3 NCBI
Target Description T22B3.3 /REP_DB=WormBase Gene ID /WP=CE03695 /TR=Q22660 /GB=CAA92617.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069815(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069815 NCBI Caenorhabditis elegans T22B3.3 (T22B3.3) mRNA, complete cds. 11/11 None
SNAP00000034101 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11703198:11703755:-1 11/11 None
GENEFINDER00000034105 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11703198:11703755:-1 11/11 None
T22B3.3 ENSEMBL cdna:known chromosome:CEL140:IV:11703198:11703755:-1 gene:T22B3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11703203-11703761(-) 76.34 100.0
Public Domain and Genome References
Gene Title
Ensembl T22B3.3
Entrez Gene 188722 Entrez gene
SwissProt Q22660 EMBL-EBI
WormBase CE03695 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92617 Hypothetical protein T22B3.3 [Caenorhabditis elegans] ref|NP_502216.1| T22B3.3 [Caenorhabditis elegans] 6.0E-69
blast CAE60041 Hypothetical protein CBG03552 [Caenorhabditis briggsae] 9.0E-44
blast CAB09420 Hypothetical protein F57A10.2 [Caenorhabditis elegans] ref|NP_506928.1| F57A10.2 [Caenorhabditis elegans] 3.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.6E-34
Sequence
Target Sequence 
>C. ELEGANS:179564_AT
ggcaggtgaaccggatttcaagctgaaagttgaacccgccgacaaaatagttatcaagtt
caagatgaacgaggagtcgatcgtccccctgacgatcacaaatccattgaaggatcgcat
ttgctacaaggtcaaatgcaccgacaatgagatcttccgtgttcgtcccccacttggctt
catcgacgctggaaaaagttcaacggtgaagatttcgctgaaggctaaagctggaatgga
cacacacagacacttctttgccatttatcacctcaccaacaatccgcctgcgaatcagaa
gaaagaagctgaaaaggcggctgaaaaaaactgtagagctctttggaaaccggagacgaa
acccgatggagttatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCAGGTGAACCGGATTTCAAGCTG 78 535 30 Antisense
TGAAAGTTGAACCCGCCGACAAAAT 214 569 53 Antisense
ATGAACGAGGAGTCGATCGTCCCCC 672 43 94 Antisense
CCCCTGACGATCACAAATCCATTGA 318 261 115 Antisense
GAAGGATCGCATTTGCTACAAGGTC 79 335 138 Antisense
GACAATGAGATCTTCCGTGTTCGTC 277 363 172 Antisense
ACTTGGCTTCATCGACGCTGGAAAA 247 105 201 Antisense
ACACACAGACACTTCTTTGCCATTT 290 111 271 Antisense
ACCAACAATCCGCCTGCGAATCAGA 443 85 304 Antisense
AGAGCTCTTTGGAAACCGGAGACGA 699 69 364 Antisense
GGAGACGAAACCCGATGGAGTTATC 141 519 381 Antisense
Sequence Source Affymetrix Proprietary Database