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[179553_at]

Description F53F8.4 /REP_DB=WormBase Gene ID /WP=CE17858 /TR=O62261 /GB=CAB04461.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 02      6grp_highest_nonorm > 02      6grp_total > 23      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3349
1 2 3 4 range
A 3369.5
(3)
P 		1588.2
(-0.4)
P 		1079.7
(-1.3)
P 		179.9
(-3)
A 		3190
B 3505.9
(3)
P 		1492.1
(-0.6)
P 		1081.1
(-1.3)
P 		156.8
(-3)
A 		3349
C 3133.5
(3)
P 		1549.8
(-0.2)
P 		979.5
(-1.4)
P 		206.6
(-3)
A 		2927
avr 3336.3
(3) 		1543.4
(-0.4) 		1046.8
(-1.4) 		181.1
(-3) 		3155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3431
1 2 3 4 5 6 range
A 3791.6
(3)
P 		2799.9
(1.1)
P 		2639.3
(0.8)
P 		1844.5
(-0.7)
P 		1078.2
(-2.2)
P 		665.1
(-3)
P 		3127
B 3591.6
(3)
P 		2881.7
(1.6)
P 		2431.9
(0.8)
P 		1921.9
(-0.2)
P 		1091.2
(-1.8)
P 		491.4
(-3)
P 		3100
C 3921.9
(3)
P 		2575.9
(0.6)
P 		2956.5
(1.3)
P 		1808.9
(-0.8)
P 		1143
(-2)
P 		593.6
(-3)
P 		3328
avr 3768.4
(3) 		2752.5
(1.1) 		2675.9
(0.9) 		1858.4
(-0.6) 		1104.1
(-2) 		583.4
(-3) 		3185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1201
1 2 3 4 range
A 1226.8
(3)
P 		344.8
(-1.4)
P 		282.2
(-1.7)
P 		26.3
(-3)
A 		1201
B 1118
(3)
P 		368.5
(-1.1)
P 		250.5
(-1.8)
P 		33.4
(-3)
A 		1085
C 1037.3
(3)
P 		445.6
(-0.5)
P 		341.4
(-1.2)
P 		34.8
(-3)
A 		1003
avr 1127.4
(3) 		386.3
(-1.1) 		291.4
(-1.6) 		31.5
(-3) 		1096

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1292
1 2 3 4 5 6 range
A 699
(0.9)
P 		995.8
(3)
P 		574.8
(-0)
P 		467.8
(-0.8)
P 		325.4
(-1.8)
P 		161.9
(-3)
P 		834
B 1249.7
(3)
P 		959.2
(1.4)
P 		590.5
(-0.7)
P 		509
(-1.2)
P 		443.3
(-1.5)
P 		183.3
(-3)
P 		1066
C 1453.7
(3)
P 		786
(-0.2)
P 		752.5
(-0.4)
P 		363.8
(-2.2)
P 		369.9
(-2.2)
P 		198.7
(-3)
P 		1255
avr 1134.1
(3) 		913.7
(1.6) 		639.3
(-0.1) 		446.9
(-1.3) 		379.5
(-1.8) 		181.3
(-3) 		953
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179553_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12863
Sequence Type Exemplar sequence
Representative Public ID F53F8.4 NCBI
Target Description F53F8.4 /REP_DB=WormBase Gene ID /WP=CE17858 /TR=O62261 /GB=CAB04461.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075596(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075596 NCBI Caenorhabditis elegans F53F8.4 (F53F8.4) mRNA, complete cds. 11/11 None
SNAP00000039378 ENSEMBL cdna:SNAP chromosome:CEL140:V:20688827:20689735:-1 11/11 None
GENEFINDER00000039383 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20686887:20689735:-1 11/11 None
F53F8.4 ENSEMBL cdna:known chromosome:CEL140:V:20688827:20689736:-1 gene:F53F8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20688826-20689735(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F8.4
Entrez Gene 186181 Entrez gene
SwissProt O62261 EMBL-EBI
WormBase CE17858 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04461 Hypothetical protein F53F8.4 [Caenorhabditis elegans] ref|NP_507997.1| F53F8.4 [Caenorhabditis elegans] 1.0E-120
blast CAB04460 Hypothetical protein F53F8.3 [Caenorhabditis elegans] ref|NP_507996.1| F53F8.3 [Caenorhabditis elegans] 1.0E-109
blast CAE61681 Hypothetical protein CBG05625 [Caenorhabditis briggsae] 1.0E-106
blast CAB04461 Hypothetical protein F53F8.4 [Caenorhabditis elegans] ref|NP_507997.1| F53F8.4 [Caenorhabditis elegans] 1.0E-100
blast CAE61681 Hypothetical protein CBG05625 [Caenorhabditis briggsae] 3.0E-87
blast AAA68772 Hypothetical protein C56C10.4 [Caenorhabditis elegans] ref|NP_495334.1| C56C10.4 [Caenorhabditis elegans] 1.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 2.0E-62
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 4.8E-46
Pfam IPR001841 EMBL-EBI Zn-finger, RING 1.4E-9
Sequence
Target Sequence 
>C. ELEGANS:179553_AT
tatggttgctacagacttcgtgcgcgtggagccaagagctaccaaccaaaaagaggacga
agcagggtcaccgttggcggatcaagcagtgaagaggctagagaagtgttgaggcaaaga
atggccatggaacgtgttcgtcgaaaggaacaacttacgtcatcagaggagttggatttg
gtgtctccaagtgtgctcagaaatagcaagtcggagcagccaatcaacccagatcgcgca
ttctacgagtgctgcattgaccgtaagcttccggacgcctgtctatctaagtgctcgttc
ggagcattcaccaaaccaaccctccaagcaatgtacttcaaacaagatccatgcccgttg
gacgctatgaaggaaatgcaattctgtgccgctcagggaagagatcatcgcgactgttgt
gcacgtaacggagttaccactactctcgctggaccaaaatgcttgagcttctgtgaccag
cg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGTTGCTACAGACTTCGTGCGC 497 655 181 Antisense
GCGCGTGGAGCCAAGAGCTACCAAC 133 287 202 Antisense
TTGGTGTCTCCAAGTGTGCTCAGAA 556 707 358 Antisense
AGATCGCGCATTCTACGAGTGCTGC 474 67 411 Antisense
GCATTGACCGTAAGCTTCCGGACGC 623 305 434 Antisense
GACGCCTGTCTATCTAAGTGCTCGT 268 381 454 Antisense
CCAACCCTCCAAGCAATGTACTTCA 91 275 496 Antisense
ATCCATGCCCGTTGGACGCTATGAA 510 39 527 Antisense
AGATCATCGCGACTGTTGTGCACGT 340 63 582 Antisense
TAACGGAGTTACCACTACTCTCGCT 542 637 606 Antisense
AATGCTTGAGCTTCTGTGACCAGCG 393 165 638 Antisense
Sequence Source Affymetrix Proprietary Database