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[179540_at]

Description K10G4.4 /REP_DB=WormBase Gene ID /WP=CE16247 /TR=O45675 /GB=CAB07259.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 5.7
(3)
A 		1.9
(-3)
A 		1.9
(-3)
A 		1.9
(-3)
A 		4
B 1.4
(-2.1)
A 		2.9
(-0.2)
A 		5.4
(3)
A 		0.7
(-3)
A 		5
C 0.6
(-2.6)
A 		3.1
(3)
A 		0.4
(-3)
A 		2.9
(2.6)
A 		3
avr 2.6
(3) 		2.6
(3) 		2.6
(3) 		1.8
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 1.7
(-2.6)
A 		1.3
(-2.8)
A 		5.2
(-0.8)
A 		0.8
(-3)
A 		12.9
(3)
A 		2.3
(-2.3)
A 		12
B 3.3
(3)
A 		0.9
(-2.1)
A 		0.5
(-3)
A 		2.7
(1.7)
A 		1
(-1.9)
A 		1.3
(-1.3)
A 		3
C 0.2
(-3)
A 		1.4
(-1.5)
A 		4.3
(2.2)
A 		1.6
(-1.2)
A 		1.5
(-1.3)
A 		4.9
(3)
A 		5
avr 1.7
(-2.2) 		1.2
(-3) 		3.3
(0.2) 		1.7
(-2.2) 		5.1
(3) 		2.8
(-0.5) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 2.1
(3)
A 		0.4
(-2.7)
A 		0.5
(-2.3)
A 		0.3
(-3)
A 		2
B 0.4
(-1.5)
A 		0.7
(0)
A 		1.3
(3)
A 		0.1
(-3)
A 		1
C 0.2
(-2.3)
A 		0.9
(3)
A 		0.1
(-3)
A 		0.5
(0)
A 		1
avr 0.9
(3) 		0.7
(1) 		0.6
(-0) 		0.3
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 0.3
(-2.8)
A 		0.5
(-2.5)
A 		1.1
(-1.5)
A 		0.2
(-3)
A 		3.9
(3)
A 		0.6
(-2.4)
A 		4
B 1.1
(3)
A 		0.3
(-1.8)
A 		0.1
(-3)
A 		0.7
(0.6)
A 		0.4
(-1.2)
A 		0.5
(-0.6)
A 		1
C 0.1
(-3)
A 		0.4
(-1.8)
A 		1.1
(1)
A 		0.3
(-2.2)
A 		0.5
(-1.4)
A 		1.6
(3)
A 		2
avr 0.5
(-2.5) 		0.4
(-3) 		0.8
(-1) 		0.4
(-3) 		1.6
(3) 		0.9
(-0.5) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179540_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13033
Sequence Type Exemplar sequence
Representative Public ID K10G4.4 NCBI
Target Description K10G4.4 /REP_DB=WormBase Gene ID /WP=CE16247 /TR=O45675 /GB=CAB07259.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074977(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074977 NCBI Caenorhabditis elegans K10G4.4 (K10G4.4) mRNA, complete cds. 11/11 A 
K10G4.4 ENSEMBL cdna:known chromosome:CEL140:V:17284955:17287746:-1 gene:K10G4.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027358 7/11 Cross Hyb Matching Probes None
GENEFINDER00000027364 7/11 Cross Hyb Matching Probes None
SNAP00000034109 7/11 Cross Hyb Matching Probes None
GENEFINDER00000034118 3/11 Cross Hyb Matching Probes None
K03D7.3 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17284954-17287746(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K10G4.4
Entrez Gene 187279 Entrez gene
SwissProt O45675 EMBL-EBI
WormBase CE16247 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07259 Hypothetical protein K10G4.4 [Caenorhabditis elegans] ref|NP_507378.1| K10G4.4 [Caenorhabditis elegans] 0.0
blast CAB07551 Hypothetical protein C01B9.1 [Caenorhabditis elegans] ref|NP_496885.1| C01B9.1 [Caenorhabditis elegans] 0.0
blast CAB04646 Hypothetical protein R10E8.6 [Caenorhabditis elegans] ref|NP_507572.1| R10E8.6 [Caenorhabditis elegans] 1.0E-158
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 5.1E-9
Sequence
Target Sequence 
>C. ELEGANS:179540_AT
tccgatttcaaattctgacaattgtcaccccatctcccaaccgatttcaaatactctcaa
atactcgatttcaaatacactcgtcaagaaaatagaagcgtcgaaaaactggtctctcaa
agttaaaatagacgagggtcaggttcagatcaacttcactcactcatccgtgccgaccaa
aaccatctgctataaaaatcacagagaaggatgtctgttggagtatgaatttcacaaagt
agtgtttgacgaggactttgtcactgcatcctgtgatgatctgaacttcgtaatgaatag
tgtgcaagagtttgacaagtttgtaattgaatctggtgagatctcagcgccaaatcgaga
agatgatccaaataactcaaatgaaacaattctaccatattggttgaataatctcgagac
acatttaaagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGATTTCAAATTCTGACAATTGT 518 593 1653 Antisense
ATTCTGACAATTGTCACCCCATCTC 705 9 1664 Antisense
AAATACTCTCAAATACTCGATTTCA 245 117 1701 Antisense
ACTCGATTTCAAATACACTCGTCAA 265 99 1715 Antisense
AGACGAGGGTCAGGTTCAGATCAAC 195 71 1782 Antisense
GACGAGGACTTTGTCACTGCATCCT 237 377 1900 Antisense
ACTTTGTCACTGCATCCTGTGATGA 445 101 1907 Antisense
AGTTTGTAATTGAATCTGGTGAGAT 245 63 1970 Antisense
AACAATTCTACCATATTGGTTGAAT 672 133 2037 Antisense
ATTGGTTGAATAATCTCGAGACACA 677 3 2051 Antisense
TAATCTCGAGACACATTTAAAGCAA 476 635 2061 Antisense
Sequence Source Affymetrix Proprietary Database