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[179537_at]

Description C50B8.4 /REP_DB=WormBase Gene ID /WP=CE23606 /TR=Q18728 /GB=CAB01131.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 48      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 80      6grp_total > 37      4grp_total > 10      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 520
1 2 3 4 range
A 200.8
(-2.3)
P 		226.4
(-2)
P 		136
(-3)
P 		655.6
(3)
P 		520
B 196.8
(-3)
P 		293.6
(-1.4)
P 		271.5
(-1.8)
P 		561
(3)
P 		364
C 242.6
(-3)
P 		273.3
(-2.5)
P 		264.5
(-2.6)
P 		595.3
(3)
P 		353
avr 213.4
(-3) 		264.4
(-2.2) 		224
(-2.8) 		604
(3) 		391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 233
(0.4)
P 		217.6
(-1)
P 		232.9
(0.4)
P 		262.4
(3)
P 		195.1
(-3)
P 		231.1
(0.2)
P 		67
B 305.7
(3)
P 		250.2
(0.5)
P 		172
(-3)
P 		227.6
(-0.5)
P 		203.8
(-1.6)
P 		271.7
(1.5)
P 		134
C 315.4
(3)
P 		233.9
(-0.1)
P 		156.7
(-3)
P 		230.7
(-0.2)
P 		243.6
(0.3)
P 		278.6
(1.6)
P 		159
avr 284.7
(3) 		233.9
(-0.1) 		187.2
(-3) 		240.2
(0.3) 		214.2
(-1.3) 		260.5
(1.5) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 73.1
(0.7)
P 		49.1
(-1.7)
P 		35.5
(-3)
P 		96
(3)
P 		61
B 62.8
(-3)
P 		72.5
(-2)
P 		62.9
(-3)
P 		119.6
(3)
P 		57
C 80.3
(-2.5)
P 		78.6
(-3)
P 		92.2
(0.7)
P 		100.4
(3)
P 		22
avr 72.1
(-1.8) 		66.7
(-2.5) 		63.5
(-3) 		105.3
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 43
(-3)
P 		77.4
(3)
P 		50.7
(-1.7)
P 		66.5
(1.1)
P 		58.9
(-0.2)
P 		56.3
(-0.7)
P 		34
B 106.4
(3)
P 		83.3
(0.9)
P 		41.8
(-3)
P 		60.3
(-1.3)
P 		82.8
(0.8)
P 		101.3
(2.5)
P 		65
C 116.9
(3)
P 		71.4
(-0.5)
P 		39.9
(-3)
P 		46.4
(-2.5)
P 		78.8
(0)
P 		93.3
(1.2)
P 		77
avr 88.8
(3) 		77.4
(1.5) 		44.1
(-3) 		57.7
(-1.2) 		73.5
(0.9) 		83.6
(2.3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179537_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13112
Sequence Type Exemplar sequence
Representative Public ID C50B8.4 NCBI
Target Description C50B8.4 /REP_DB=WormBase Gene ID /WP=CE23606 /TR=Q18728 /GB=CAB01131.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073962(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073962 NCBI Caenorhabditis elegans C50B8.4 (C50B8.4) mRNA, complete cds. 11/11 None
SNAP00000010253 ENSEMBL cdna:SNAP chromosome:CEL140:V:13574728:13575921:1 11/11 None
GENEFINDER00000010261 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13574728:13575921:1 11/11 None
C50B8.4 ENSEMBL cdna:known chromosome:CEL140:V:13574710:13575935:1 gene:C50B8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13574727-13575921(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50B8.4
Entrez Gene 179842 Entrez gene
SwissProt Q18728 EMBL-EBI
WormBase CE23606 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01131 Hypothetical protein C50B8.4 [Caenorhabditis elegans] ref|NP_506363.1| C50B8.4 [Caenorhabditis elegans] 1.0E-59
blast CAE66289 Hypothetical protein CBG11536 [Caenorhabditis briggsae] 9.0E-50
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506363.1 1 108-130
C50B8.4 1 108-130
SNAP00000010253 1 108-130
GENEFINDER00000010261 1 108-130
Sequence
Target Sequence 
>C. ELEGANS:179537_AT
atcaaccacaacaattcaatcagtatggtaataataacaacaattactataatataatcg
ctgaaacaactacattcaatccaaatccaaacgcttcaattgattttgtcgtgactcaag
tggatcgattgtccaatcagattaacagtttagttgctgatggagaaacagcaggtctac
aaattgctgatcgtggggtcgaaacttttaatcgaatgtcaaatgctttcaataatggaa
caatacaaaatatcaatcgaatcggggacattgcaagtgaaaagtttcacaactggccag
taacagaactcattattatggtaactattggtgcagttttggttgtgataactttaatat
tcctctttgtcttatgtggagaacgcgtcactttgtatcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAACCACAACAATTCAATCAGTA 203 27 23 Antisense
TTCAATCCAAATCCAAACGCTTCAA 406 685 97 Antisense
ACGCTTCAATTGATTTTGTCGTGAC 57 93 113 Antisense
GACTCAAGTGGATCGATTGTCCAAT 535 373 135 Antisense
GCAGGTCTACAAATTGCTGATCGTG 240 311 193 Antisense
GCTGATCGTGGGGTCGAAACTTTTA 670 291 208 Antisense
AATCGAATCGGGGACATTGCAAGTG 456 161 277 Antisense
ACAACTGGCCAGTAACAGAACTCAT 535 111 311 Antisense
TAACTTTAATATTCCTCTTTGTCTT 385 639 371 Antisense
TATTCCTCTTTGTCTTATGTGGAGA 271 665 380 Antisense
GGAGAACGCGTCACTTTGTATCGGA 429 519 400 Antisense
Sequence Source Affymetrix Proprietary Database