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[179526_at]

Description ZK218.3 /REP_DB=WormBase Gene ID /WP=CE16703 /TR=O45996 /GB=CAB04982.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 28      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 31.7
(3)
A 		11.1
(-2.8)
A 		10.3
(-3)
A 		10.7
(-2.9)
A 		21
B 15.7
(0.3)
A 		26.4
(3)
A 		16.2
(0.4)
A 		2.7
(-3)
A 		24
C 5.6
(-3)
A 		16.3
(-1.6)
A 		11.7
(-2.2)
A 		50.4
(3)
A 		45
avr 17.7
(0.5) 		17.9
(0.6) 		12.7
(-3) 		21.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 4.2
(-3)
A 		8.8
(3)
A 		5.8
(-0.9)
A 		6.8
(0.4)
A 		7.3
(1)
A 		5.6
(-1.2)
A 		5
B 9.9
(0)
A 		17.4
(3)
A 		5.4
(-1.8)
A 		2.3
(-3)
A 		6.6
(-1.3)
A 		11.5
(0.7)
A 		15
C 12.5
(3)
A 		10.1
(1.1)
A 		6.9
(-1.5)
A 		5.9
(-2.3)
A 		6.4
(-1.9)
A 		5
(-3)
A 		8
avr 8.9
(0.3) 		12.1
(3) 		6
(-2.2) 		5
(-3) 		6.8
(-1.5) 		7.4
(-1) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 11.5
(3)
A 		2.4
(-2.5)
A 		2.7
(-2.3)
A 		1.6
(-3)
A 		10
B 5
(1.5)
A 		6.5
(3)
A 		3.8
(0.3)
A 		0.6
(-3)
A 		6
C 1.8
(-3)
A 		4.7
(-0.4)
A 		4.1
(-0.9)
A 		8.5
(3)
A 		7
avr 6.1
(3) 		4.5
(-0.7) 		3.5
(-3) 		3.6
(-2.8) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 0.8
(-3)
A 		3.1
(3)
A 		1.3
(-1.7)
A 		1.7
(-0.7)
A 		2.2
(0.7)
A 		1.4
(-1.4)
A 		2
B 3.5
(0.3)
A 		5.8
(3)
A 		1.3
(-2.2)
A 		0.6
(-3)
A 		2.7
(-0.6)
A 		4.3
(1.3)
A 		5
C 4.6
(3)
A 		3.1
(0.4)
A 		1.8
(-1.9)
A 		1.2
(-3)
A 		2.1
(-1.4)
A 		1.7
(-2.1)
A 		3
avr 3
(0.9) 		4
(3) 		1.5
(-2.4) 		1.2
(-3) 		2.3
(-0.6) 		2.5
(-0.2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179526_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13465
Sequence Type Exemplar sequence
Representative Public ID ZK218.3 NCBI
Target Description ZK218.3 /REP_DB=WormBase Gene ID /WP=CE16703 /TR=O45996 /GB=CAB04982.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074930(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074930 NCBI Caenorhabditis elegans ZK218.3 (ZK218.3) mRNA, complete cds. 11/11 None
SNAP00000041062 ENSEMBL cdna:SNAP chromosome:CEL140:V:17100505:17101344:-1 11/11 None
GENEFINDER00000041074 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17100505:17101344:-1 11/11 None
ZK218.3 ENSEMBL cdna:known chromosome:CEL140:V:17100505:17101344:-1 gene:ZK218.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17100504-17101344(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK218.3
Entrez Gene 180138 Entrez gene
SwissProt O45996 EMBL-EBI
WormBase CE16703 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04982 Hypothetical protein ZK218.3 [Caenorhabditis elegans] ref|NP_507331.1| ZK218.3 [Caenorhabditis elegans] 1.0E-150
blast CAB04987 Hypothetical protein ZK218.7 [Caenorhabditis elegans] ref|NP_507335.1| ZK218.7 [Caenorhabditis elegans] 1.0E-111
blast CAB04986 Hypothetical protein ZK218.5 [Caenorhabditis elegans] ref|NP_507333.1| ZK218.5 [Caenorhabditis elegans] 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.3
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.4E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.2E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-11
Sequence
Target Sequence 
>C. ELEGANS:179526_AT
acaaactgtgtcaacgcctatgctgactctacatgtgcatctctctacgctatcaccaca
gatgagccagcaccagtgccaggaacaagcacacctcgcccattgaaatgcttcaccgcc
ggagaagatgctatcggtgcagtagatccagatcttgtaagagctgcagttagtacatgc
ccaaagacttgtggattctgttgccaaagcgcagactacaattgtcctaatgttcagttc
ccacgtctcaattgtgccaccatccttccatcccaatgcacaaatcaagaatggcgtgtc
atcattgcccaagattgtccttcagcgtgcggattctgtaatcagggaggatgtgttgat
gcagtaatgggatgtgcaaatgacatctccatttgtaacgccgtcggaatgcaaaccttt
gtcaacacgaactgccaaagaacttgcaacagatgctcctcaaccacagttaccggaggt
tgtacctacaatcgtgactcaagcaccgcttgtgctgcttgggctgtcaatggattctgc
cagaacgacttctacactcctgcccaaaggaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAACTGTGTCAACGCCTATGCTG 515 111 151 Antisense
ACGCTATCACCACAGATGAGCCAGC 252 81 197 Antisense
GAGCTGCAGTTAGTACATGCCCAAA 153 387 311 Antisense
GTCCTTCAGCGTGCGGATTCTGTAA 287 475 467 Antisense
ATGACATCTCCATTTGTAACGCCGT 252 45 530 Antisense
AAACCTTTGTCAACACGAACTGCCA 636 125 563 Antisense
TGCTCCTCAACCACAGTTACCGGAG 220 571 604 Antisense
ACAATCGTGACTCAAGCACCGCTTG 120 115 638 Antisense
TGGGCTGTCAATGGATTCTGCCAGA 618 553 670 Antisense
TTCTGCCAGAACGACTTCTACACTC 33 695 685 Antisense
CTTCTACACTCCTGCCCAAAGGAAA 366 219 699 Antisense
Sequence Source Affymetrix Proprietary Database