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[179516_at]

Description W10C6.1 /REP_DB=WormBase Gene ID /WP=CE21288 /TR=O18191 /GB=CAB16467.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 82      6grp_total > 49      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 448.8
(3)
P 		255
(-2.6)
P 		264
(-2.4)
P 		242.9
(-3)
P 		206
B 431.3
(3)
P 		305.2
(-1.1)
P 		247.3
(-3)
P 		286.7
(-1.7)
P 		184
C 447.3
(3)
P 		230.9
(-3)
P 		260.4
(-2.2)
P 		265.8
(-2)
P 		216
avr 442.5
(3) 		263.7
(-2.8) 		257.2
(-3) 		265.1
(-2.7) 		185

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 328
1 2 3 4 5 6 range
A 356.2
(-1.2)
P 		318.2
(-2.1)
P 		278.2
(-3)
P 		341.9
(-1.6)
P 		324
(-2)
P 		542.8
(3)
P 		265
B 404.2
(-0.2)
P 		411.7
(-0)
P 		252.4
(-3)
P 		266.8
(-2.7)
P 		406
(-0.2)
P 		575.9
(3)
P 		324
C 494.4
(3)
P 		394.3
(0.6)
P 		398.9
(0.7)
P 		247.5
(-3)
P 		373.7
(0.1)
P 		289.2
(-2)
P 		247
avr 418.3
(1.3) 		374.7
(-0.1) 		309.8
(-2.2) 		285.4
(-3) 		367.9
(-0.3) 		469.3
(3) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 163.4
(3)
P 		55.3
(-2.1)
P 		69
(-1.4)
P 		35.6
(-3)
P 		128
B 137.5
(3)
P 		75.4
(-1.6)
P 		57.3
(-3)
P 		61.1
(-2.7)
P 		80
C 148.1
(3)
P 		66.4
(-1.7)
P 		90.7
(-0.3)
P 		44.8
(-3)
P 		103
avr 149.7
(3) 		65.7
(-1.9) 		72.3
(-1.5) 		47.2
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 65.7
(-2.6)
P 		113.2
(1.4)
P 		60.6
(-3)
P 		86.7
(-0.8)
P 		97.8
(0.1)
P 		132.2
(3)
P 		72
B 140.6
(0.1)
P 		137
(-0)
P 		61.3
(-3)
P 		70.7
(-2.6)
P 		164.9
(1)
P 		214.8
(3)
P 		154
C 183.3
(3)
P 		120.3
(0.2)
P 		101.5
(-0.7)
P 		49.8
(-3)
P 		120.9
(0.2)
P 		96.8
(-0.9)
P 		134
avr 129.9
(1.6) 		123.5
(1.1) 		74.5
(-2.6) 		69.1
(-3) 		127.9
(1.5) 		147.9
(3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179516_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2790
Sequence Type Exemplar sequence
Representative Public ID W10C6.1 NCBI
Target Description W10C6.1 /REP_DB=WormBase Gene ID /WP=CE21288 /TR=O18191 /GB=CAB16467.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063982(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063982 NCBI Caenorhabditis elegans Metaphase-to-Anaphase Transition defect family member (mat-2) (mat-2) mRNA, complete cds. 11/11 None
SNAP00000018107 ENSEMBL cdna:SNAP chromosome:CEL140:II:11113247:11120627:1 11/11 None
GENEFINDER00000018108 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11113247:11120627:1 11/11 None
W10C6.1 ENSEMBL cdna:known chromosome:CEL140:II:11113247:11120701:1 gene:W10C6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11113243-11120624(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mat-2
Ensembl W10C6.1
Entrez Gene 174698 Entrez gene
SwissProt O18191 EMBL-EBI
WormBase CE21288 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5680 anaphase-promoting complex inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5488 binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB16467 Hypothetical protein W10C6.1 [Caenorhabditis elegans] ref|NP_496383.1| Metaphase-to-Anaphase Transition defect family member (mat-2) [Caenorhabditis elegans] 0.0
blast CAE59567 Hypothetical protein CBG02964 [Caenorhabditis briggsae] 0.0
blast XP_230589 PREDICTED: similar to tsg24 [Rattus norvegicus] 5.0E-79
Sequence
Target Sequence 
>C. ELEGANS:179516_AT
tctcgtgccattcgacattgccgagagctgtgtggttgaagttgatgtgacgattgttat
gaagccaaaagatggaaatgagccgattgtctacaaagagaaggctccgtatcttcttcc
accactcgaggaccttcagtcaatttcaattggaggtggcaactatcaattggtgcatat
cagtctacaatcagaagatcaggtgaaagtgatgaaggatattatgacaattggacaagg
acgcgtgatgctcaaaagatatggtgtagattcgtcggaaatgaagataaaagaagcaac
aacactgtatgatgatactcctagcttgatgagcatgtttaataatgaagatactgctgt
ggaacttgacgagtatgagattcagtgtatgatggagaagattgacgaagggattaactt
gaatagctcagacgagtatccaaatgttcaaattgaattgagttgtgtcagagatgtcac
tgaacgaacaactatggatttggcacaattacagaaacgatccttgaaactattaagtga
aagtcttgatctctggcaggatgaggtcaacgtttcaaacacaatcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCGTGCCATTCGACATTGCCGAG 256 611 3774 Antisense
GACATTGCCGAGAGCTGTGTGGTTG 356 371 3787 Antisense
TGATGTGACGATTGTTATGAAGCCA 461 567 3816 Antisense
GTCTACAAAGAGAAGGCTCCGTATC 590 469 3862 Antisense
CACCACTCGAGGACCTTCAGTCAAT 698 201 3893 Antisense
GGAGGTGGCAACTATCAATTGGTGC 45 517 3925 Antisense
TGGTGCATATCAGTCTACAATCAGA 186 549 3944 Antisense
TGGACAAGGACGCGTGATGCTCAAA 2 549 4005 Antisense
GATATGGTGTAGATTCGTCGGAAAT 653 425 4031 Antisense
GTATGATGATACTCCTAGCTTGATG 73 455 4080 Antisense
GGTCAACGTTTCAAACACAATCAAC 16 505 4338 Antisense
Sequence Source Affymetrix Proprietary Database