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[179515_at]

Description T27F6.7 /REP_DB=WormBase Gene ID /WP=CE16516 /TR=O45871 /GB=CAB04886.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 89      4grp_high_8_8 > 43      6grp_total > 99      4grp_total > 53      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 235
1 2 3 4 range
A 391.6
(3)
P 		308.7
(0)
P 		225.2
(-3)
A 		251.8
(-2)
P 		166
B 318.6
(0.5)
A 		356.2
(3)
P 		329.4
(1.2)
P 		264.9
(-3)
A 		91
C 368.3
(2.2)
P 		399.3
(3)
P 		290.2
(0.2)
P 		164.8
(-3)
A 		235
avr 359.5
(3) 		354.7
(2.8) 		281.6
(-0.5) 		227.2
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 230
1 2 3 4 5 6 range
A 269.5
(-2)
A 		305.7
(-1)
P 		306.3
(-1)
A 		231.8
(-3)
A 		455.7
(3)
P 		361.2
(0.5)
P 		224
B 392.4
(0.6)
P 		289.8
(-2.9)
P 		285.9
(-3)
P 		331.3
(-1.5)
P 		324.2
(-1.7)
P 		462.1
(3)
P 		176
C 329.3
(0.2)
P 		263.7
(-3)
M 		288.8
(-1.8)
M 		306.4
(-0.9)
A 		388.3
(3)
P 		312.1
(-0.7)
P 		125
avr 330.4
(-0.4) 		286.4
(-3) 		293.7
(-2.6) 		289.8
(-2.8) 		389.4
(3) 		378.5
(2.4) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 142.6
(3)
P 		67
(-1.3)
P 		58.9
(-1.8)
A 		36.9
(-3)
P 		106
B 101.6
(3)
A 		88
(1.2)
P 		76.3
(-0.4)
P 		56.4
(-3)
A 		45
C 121.9
(3)
P 		114.8
(2.5)
P 		101.1
(1.7)
P 		27.8
(-3)
A 		94
avr 122
(3) 		89.9
(0.6) 		78.8
(-0.2) 		40.4
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 49.7
(-3)
A 		108.7
(1)
P 		66.7
(-1.8)
A 		58.8
(-2.4)
A 		137.5
(3)
P 		87.9
(-0.4)
P 		88
B 136.5
(0.9)
P 		96.5
(-1.4)
P 		69.4
(-3)
P 		87.8
(-1.9)
P 		131.7
(0.6)
P 		172.3
(3)
P 		103
C 122.1
(2.7)
P 		80.5
(-1.2)
M 		73.5
(-1.9)
M 		61.6
(-3)
A 		125.6
(3)
P 		104.5
(1)
P 		64
avr 102.8
(0.2) 		95.2
(-0.5) 		69.9
(-3) 		69.4
(-3) 		131.6
(3) 		121.6
(2) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179515_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1883
Sequence Type Exemplar sequence
Representative Public ID T27F6.7 NCBI
Target Description T27F6.7 /REP_DB=WormBase Gene ID /WP=CE16516 /TR=O45871 /GB=CAB04886.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060769(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060769 NCBI Caenorhabditis elegans T27F6.7 (T27F6.7) mRNA, complete cds. 11/11 None
SNAP00000032604 ENSEMBL cdna:SNAP chromosome:CEL140:I:12502954:12505315:1 11/11 None
GENEFINDER00000032605 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12502954:12505315:1 11/11 None
T27F6.7.2 ENSEMBL cdna:known chromosome:CEL140:I:12502945:12505450:1 gene:T27F6.7 11/11 None
T27F6.7.1 ENSEMBL cdna:known chromosome:CEL140:I:12502947:12505450:1 gene:T27F6.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12502884-12505246(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27F6.7
Entrez Gene 173119 Entrez gene
SwissProt O45871 EMBL-EBI
WormBase CE16516 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04886 Hypothetical protein T27F6.7 [Caenorhabditis elegans] ref|NP_493170.1| T27F6.7 [Caenorhabditis elegans] 1.0E-137
blast CAE69320 Hypothetical protein CBG15387 [Caenorhabditis briggsae] 4.0E-85
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493170.1 4 60-79,94-116,129-148,168-190
T27F6.7.2 4 60-79,94-116,129-148,168-190
T27F6.7.1 4 60-79,94-116,129-148,168-190
SNAP00000032604 4 60-79,94-116,129-148,168-190
GENEFINDER00000032605 4 60-79,94-116,129-148,168-190
Sequence
Target Sequence 
>C. ELEGANS:179515_AT
gaataccgcgcgttttttacacatgcgacacgggcggccgccattgccgctggctttggg
ctcagctgctcgctcttctcgtttgcaatgttcttgttcgaattccgatgggatgatcat
ggccgaggtttcgatgcgggcactgttgttgcctccctcctgttcctgataatcggcgtc
ctagtccattggcaagttataatcggaataaaaaaggagtctgcggtgcatatgatccca
gcaatcatggtctacacgtttttgattgtcgtcgagctgattgtctacgttttggccgca
aatcacctcgtcaactcggcttccctgttcgcctatccaccggacaaacagacgccaacg
ttcctcacattatcgattttctacctgacacttgtcggaattcagacttcgatgcttttg
tcgattaccaaggctcggaactactacgaagccaaggatatccataaaaaggaggttcgg
gttgccgaacatgggaaacgcctcaatccccaaatgcaaattgtctacgtcaaagacggt
gaccaggtggaccccatgtcgaccgacggtgagccggtgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATACCGCGCGTTTTTTACACATG 181 325 157 Antisense
CTCTTCTCGTTTGCAATGTTCTTGT 124 231 229 Antisense
ATGGCCGAGGTTTCGATGCGGGCAC 542 53 275 Antisense
TCCTGATAATCGGCGTCCTAGTCCA 126 593 320 Antisense
GTCTGCGGTGCATATGATCCCAGCA 473 471 375 Antisense
GATTGTCGTCGAGCTGATTGTCTAC 622 433 420 Antisense
GTCTACGTTTTGGCCGCAAATCACC 526 469 439 Antisense
CCAACGTTCCTCACATTATCGATTT 602 275 511 Antisense
ATCGATTTTCTACCTGACACTTGTC 566 35 528 Antisense
ATGGGAAACGCCTCAATCCCCAAAT 708 39 647 Antisense
TGTCGACCGACGGTGAGCCGGTGAC 556 555 713 Antisense
Sequence Source Affymetrix Proprietary Database