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[179505_at]

Description F56A8.1 /REP_DB=WormBase Gene ID /WP=CE16121 /TR=O45572 /GB=CAB05741.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 41      4grp_total > 52      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 119.1
(-0.1)
M 		157.1
(3)
P 		125.3
(0.4)
A 		83.6
(-3)
A 		74
B 127.3
(2.2)
A 		135.1
(3)
P 		117.6
(1.1)
P 		79.9
(-3)
A 		55
C 148.8
(3)
P 		137.4
(1.4)
A 		136.7
(1.3)
P 		105.4
(-3)
A 		43
avr 131.7
(1.7) 		143.2
(3) 		126.5
(1.1) 		89.6
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 156
(2.2)
A 		162.3
(3)
M 		157.2
(2.4)
P 		140.5
(0.2)
P 		115
(-3)
A 		130.6
(-1)
P 		47
B 163.2
(2.2)
M 		115.9
(-3)
A 		170.2
(3)
P 		154.7
(1.3)
P 		141.7
(-0.1)
P 		120
(-2.5)
P 		54
C 142.9
(0.9)
P 		128.5
(-0.8)
M 		148
(1.4)
A 		161.9
(3)
P 		108.6
(-3)
P 		133.9
(-0.2)
P 		53
avr 154
(2.3) 		135.6
(-0.7) 		158.5
(3) 		152.4
(2) 		121.8
(-3) 		128.2
(-2) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 43.4
(3)
M 		34.1
(1.2)
P 		32.8
(1)
A 		12.2
(-3)
A 		31
B 40.6
(3)
A 		33.4
(1.2)
P 		27.2
(-0.4)
P 		17
(-3)
A 		24
C 49.2
(3)
P 		39.5
(1.1)
A 		47.6
(2.7)
P 		17.8
(-3)
A 		31
avr 44.4
(3) 		35.7
(1.2) 		35.9
(1.2) 		15.7
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 28.8
(-3)
A 		57.7
(3)
M 		34.2
(-1.9)
P 		35.6
(-1.6)
P 		34.7
(-1.8)
A 		31.8
(-2.4)
P 		29
B 56.8
(2.7)
M 		38.6
(-3)
A 		41.3
(-2.1)
P 		41
(-2.2)
P 		57.6
(3)
P 		44.8
(-1)
P 		19
C 53
(3)
P 		39.2
(-1.1)
M 		37.7
(-1.5)
A 		32.6
(-3)
P 		35.1
(-2.3)
P 		44.8
(0.6)
P 		20
avr 46.2
(3) 		45.2
(2.4) 		37.7
(-2.2) 		36.4
(-3) 		42.5
(0.7) 		40.5
(-0.5) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179505_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6167
Sequence Type Exemplar sequence
Representative Public ID F56A8.1 NCBI
Target Description F56A8.1 /REP_DB=WormBase Gene ID /WP=CE16121 /TR=O45572 /GB=CAB05741.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067328(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067328 NCBI Caenorhabditis elegans F56A8.1 (F56A8.1) mRNA, complete cds. 11/11 None
SNAP00000043509 ENSEMBL cdna:SNAP chromosome:CEL140:III:13254204:13255814:1 11/11 None
GENEFINDER00000043519 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13254204:13255814:1 11/11 None
F56A8.1 ENSEMBL cdna:known chromosome:CEL140:III:13247454:13255901:1 gene:F56A8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13247470-13255810(+) 99.11 100.0
Public Domain and Genome References
Gene Title
Ensembl F56A8.1
Entrez Gene 176739 Entrez gene
SwissProt O45572 EMBL-EBI
WormBase CE16121 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:241918_AT transmembrane protein 16G hs
HG-U133B:241918_AT transmembrane protein 16G hs
U133_X3P:HS.147924.0.A1_3P_AT transmembrane protein 16G hs
HG-U95C:62387_AT transmembrane protein 16G hs
HG-U95E:84098_AT Transmembrane protein 16G hs
Protein Similarities
Method ID Description E-Value
blast CAB05741 Hypothetical protein F56A8.1 [Caenorhabditis elegans] ref|NP_499729.1| F56A8.1 [Caenorhabditis elegans] 0.0
blast CAE60063 Hypothetical protein CBG03579 [Caenorhabditis briggsae] 0.0
blast CAB05741 Hypothetical protein F56A8.1 [Caenorhabditis elegans] ref|NP_499729.1| F56A8.1 [Caenorhabditis elegans] 1.0E-104
blast CAB05741 Hypothetical protein F56A8.1 [Caenorhabditis elegans] ref|NP_499729.1| F56A8.1 [Caenorhabditis elegans] 1.0E-101
blast CAE60063 Hypothetical protein CBG03579 [Caenorhabditis briggsae] 5.0E-78
blast CAE60063 Hypothetical protein CBG03579 [Caenorhabditis briggsae] 2.0E-76
blast XP_792017 PREDICTED: similar to putative protein, with at least 7 transmembrane domains, 2 coiled coil-4 domains, of fungal and metazoan origin (3O345) [Strongylocentrotus purpuratus] 4.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR007632 EMBL-EBI Protein of unknown function DUF590 4.2E-69
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499729.1 9 271-293,347-364,421-443,463-485,505-527,609-631,648-670,674-696,703-725
F56A8.1 9 271-293,347-364,421-443,463-485,505-527,609-631,648-670,674-696,703-725
Sequence
Target Sequence 
>C. ELEGANS:179505_AT
gaaacgcattcggatccagctaaattgaaaacgaaagaagcgcccgagcatattgatcac
aagaaattattcaccgaaagcttcaaacaacggagaatcgagcatacgaccgcaattgag
tcgattaaaaaaattggaaaagacaagcggagattgtttttggaagatgttttgaaaaat
gtgcaaaacctgctgaaagagtcgcgagaagctttggaacggaccgcccatcgatcggat
tcgccgttcccacacggtagtgagctgctcaaggatgcactttccaagctccacgagaac
atcgccttcttcacagacctctcactagcgttctcgaaatttttcgaaacgaaaatccaa
aaggatcggaaattgaagactttgatcgtctgggcctacaattattctatcaaaactggc
atttttgatgaggaaactgaaaagaaagtgcaactaatggctcaacaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACGCATTCGGATCCAGCTAAAT 64 359 2551 Antisense
AGAAGCGCCCGAGCATATTGATCAC 576 73 2586 Antisense
GGAGAATCGAGCATACGACCGCAAT 492 519 2642 Antisense
AAACCTGCTGAAAGAGTCGCGAGAA 497 123 2736 Antisense
GTTCCCACACGGTAGTGAGCTGCTC 329 441 2796 Antisense
TCAAGGATGCACTTTCCAAGCTCCA 145 633 2819 Antisense
TTCCAAGCTCCACGAGAACATCGCC 601 695 2832 Antisense
CCTCTCACTAGCGTTCTCGAAATTT 20 269 2868 Antisense
GAAGACTTTGATCGTCTGGGCCTAC 673 339 2925 Antisense
GTCTGGGCCTACAATTATTCTATCA 704 471 2938 Antisense
AAGTGCAACTAATGGCTCAACAACA 418 159 2996 Antisense
Sequence Source Affymetrix Proprietary Database