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[179469_s_at]

Description F35E8.4 /REP_DB=WormBase Gene ID /WP=CE15953 /TR=O46356 /GB=CAB04300.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 88      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_total > 67      4grp_total > 45      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 207
(3)
P
193.3
(2.1)
P
115.1
(-3)
P
115.4
(-3)
P
92
B 217.6
(3)
P
187.4
(1.2)
P
119.5
(-3)
P
131.3
(-2.3)
P
98
C 215.8
(3)
P
191.3
(1.7)
P
106.8
(-3)
P
150.2
(-0.6)
P
109
avr 213.5
(3)
190.7
(1.6)
113.8
(-3)
132.3
(-1.9)
100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 198.6
(2)
P
70.9
(-3)
P
145.2
(-0.1)
P
135.6
(-0.5)
P
185.6
(1.5)
P
223.9
(3)
P
153
B 153.1
(-1.3)
P
135.7
(-3)
P
170
(0.4)
P
139.8
(-2.6)
P
136.5
(-2.9)
P
195.5
(3)
P
60
C 87.3
(-2.8)
P
147.7
(0.1)
P
147.5
(0.1)
P
83.7
(-3)
A
93.5
(-2.5)
A
206.7
(3)
P
123
avr 146.3
(-1.1)
118.1
(-3)
154.2
(-0.6)
119.7
(-2.9)
138.5
(-1.6)
208.7
(3)
91
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 75.4
(3)
P
42
(-0.4)
P
30.1
(-1.6)
P
16.9
(-3)
P
59
B 69.4
(3)
P
46.3
(-0.3)
P
27.7
(-3)
P
28
(-3)
P
42
C 71.4
(3)
P
55
(0.9)
P
37.2
(-1.5)
P
25.3
(-3)
P
46
avr 72.1
(3)
47.8
(0)
31.7
(-2)
23.4
(-3)
49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 36.6
(-0.8)
P
25.2
(-3)
P
31.6
(-1.8)
P
34.4
(-1.2)
P
56
(3)
P
54.5
(2.7)
P
31
B 53.3
(-0.3)
P
45.2
(-1.6)
P
41.3
(-2.3)
P
37
(-3)
P
55.4
(0.1)
P
72.9
(3)
P
36
C 32.4
(-1.2)
P
45.1
(0.2)
P
37.5
(-0.6)
P
16.8
(-3)
A
30.3
(-1.5)
A
69.2
(3)
P
52
avr 40.8
(-1.1)
38.5
(-1.5)
36.8
(-1.8)
29.4
(-3)
47.2
(-0)
65.5
(3)
36
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179469_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13240
Sequence Type Exemplar sequence
Representative Public ID F35E8.4 NCBI
Target Description F35E8.4 /REP_DB=WormBase Gene ID /WP=CE15953 /TR=O46356 /GB=CAB04300.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074576(11), NM_074579(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_074576 NCBI Caenorhabditis elegans F35E8.1 (F35E8.1) mRNA, complete cds. 11/11 None
NM_074579 NCBI Caenorhabditis elegans F35E8.4 (F35E8.4) mRNA, complete cds. 11/11 None
SNAP00000019508 ENSEMBL cdna:SNAP chromosome:CEL140:V:15906776:15907674:-1 11/11 None
SNAP00000019511 ENSEMBL cdna:SNAP chromosome:CEL140:V:15912715:15913613:-1 11/11 None
GENEFINDER00000019521 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15906776:15907674:-1 11/11 None
GENEFINDER00000019529 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15912157:15913613:-1 9/11 None
F35E8.1 ENSEMBL cdna:known chromosome:CEL140:V:15906776:15907674:-1 gene:F35E8.1 11/11 None
F35E8.4 ENSEMBL cdna:known chromosome:CEL140:V:15912715:15913613:-1 gene:F35E8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15912714-15913613(-) 100.0 100.0
chrV:15906775-15907674(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl F35E8.1
F35E8.4
Entrez Gene 185292 Entrez gene
185295 Entrez gene
SwissProt O46356 EMBL-EBI
WormBase CE15953 Wormbase
CE15953 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04300 Hypothetical protein F35E8.4 [Caenorhabditis elegans] emb|CAB04294.1| Hypothetical protein F35E8.1 [Caenorhabditis elegans] ref|NP_506980.1| F35E8.4 [Caenorhabditis elegans] ref|NP_506977.1| F35E8.1 [Caenorhabditis elegans] 1.0E-138
blast CAB04300 Hypothetical protein F35E8.4 [Caenorhabditis elegans] emb|CAB04294.1| Hypothetical protein F35E8.1 [Caenorhabditis elegans] ref|NP_506980.1| F35E8.4 [Caenorhabditis elegans] ref|NP_506977.1| F35E8.1 [Caenorhabditis elegans] 1.0E-107
blast AAC69054 Hypothetical protein T05B4.3 [Caenorhabditis elegans] ref|NP_504152.1| T05B4.3 [Caenorhabditis elegans] 2.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.0E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-12
Sequence
Target Sequence
>C. ELEGANS:179469_S_AT
tcctaaccgattcctcgtgtgatgccttatttccacccatagctgcagccgatggaaatc
catctgcaggaaaagatcaagctagagctttcagatgttactcaacagctacggctttac
cggctccaatagttgaagatgtcaagagagttgccataaacggttgtccaaagacttgtg
gttattgttgtcaaacgagtgaatttaactgcgcaaatgccgagtttccaagcatcaaat
gttccgatatcacaccagcccaatgtcggtcacctgtctggagaaccgtcatagcacaag
attgcccatctgcctgtggattctgcaatgaaggaggatgtgtcgatgctataactgact
gcggcgatgatctcagtatttgtcttaatgtcaaaatgcaagattttgtcaaccaatact
gccaaaaaacgtgtaagagatgctccgctgccacaacagttgctccgccggtaaccaaca
actgcaccgtgttccctccggatacaagaactgcctgcaaagcctgggctcgcaatggat
tct


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
TCCTAACCGATTCCTCGTGTGATGC 303 591 77 Antisense
CCTCGTGTGATGCCTTATTTCCACC 526 267 89 Antisense
GTTACTCAACAGCTACGGCTTTACC 696 443 173 Antisense
GGCTTTACCGGCTCCAATAGTTGAA 211 535 189 Antisense
CTGCGCAAATGCCGAGTTTCCAAGC 262 237 285 Antisense
CAAATGTTCCGATATCACACCAGCC 259 193 312 Antisense
AATGTCGGTCACCTGTCTGGAGAAC 538 161 338 Antisense
ATGCTATAACTGACTGCGGCGATGA 293 43 422 Antisense
AACGTGTAAGAGATGCTCCGCTGCC 653 143 504 Antisense
CCCTCCGGATACAAGAACTGCCTGC 408 259 570 Antisense
AAAGCCTGGGCTCGCAATGGATTCT 230 125 595 Antisense
Sequence Source Affymetrix Proprietary Database