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[179452_at]

Description Y6E2A.4 /REP_DB=WormBase Gene ID /WP=CE16581 /TR=O45969 /GB=CAA15963.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 78
(-1.9)
A 		41.2
(-3)
A 		67.7
(-2.2)
A 		246.3
(3)
P 		205
B 59.6
(-2.8)
A 		52.8
(-3)
A 		52.8
(-3)
A 		260.2
(3)
P 		207
C 64.9
(-2.3)
A 		53.2
(-2.7)
A 		43
(-3)
A 		232.2
(3)
P 		189
avr 67.5
(-2.4) 		49.1
(-3) 		54.5
(-2.8) 		246.2
(3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 394
1 2 3 4 5 6 range
A 68
(-2.7)
P 		53.9
(-3)
A 		72.4
(-2.7)
P 		161.2
(-1)
P 		323.3
(2)
P 		380.3
(3)
P 		326
B 72
(-3)
A 		75.9
(-2.9)
A 		87
(-2.8)
M 		242.8
(-0.2)
P 		440.2
(3)
P 		366.1
(1.8)
P 		368
C 45.8
(-3)
A 		73.6
(-2.4)
A 		89.4
(-2)
P 		220.4
(0.9)
P 		295.4
(2.6)
P 		311.6
(3)
P 		266
avr 61.9
(-3) 		67.8
(-2.9) 		82.9
(-2.6) 		208.1
(0) 		353
(3) 		352.7
(3) 		291
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 28.4
(1.3)
A 		8.9
(-3)
A 		17.7
(-1.1)
A 		36.1
(3)
P 		27
B 19
(-2.1)
A 		13
(-2.9)
A 		12.2
(-3)
A 		55.4
(3)
P 		43
C 21.5
(-1.4)
A 		15.3
(-2.9)
A 		15
(-3)
A 		39.2
(3)
P 		24
avr 23
(-1) 		12.4
(-3) 		15
(-2.5) 		43.6
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 12.5
(-3)
P 		19.2
(-2.5)
A 		15.8
(-2.8)
P 		40.9
(-1)
P 		97.5
(3)
P 		92.6
(2.7)
P 		85
B 25
(-2.9)
A 		25.3
(-2.8)
A 		21.1
(-3)
M 		64.3
(-1.4)
P 		178.9
(3)
P 		136.5
(1.4)
P 		158
C 17
(-3)
A 		22.5
(-2.6)
A 		22.7
(-2.6)
P 		44.3
(-1.1)
P 		95.6
(2.4)
P 		104.3
(3)
P 		87
avr 18.2
(-3) 		22.3
(-2.8) 		19.9
(-2.9) 		49.8
(-1.2) 		124
(3) 		111.1
(2.3) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179452_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14620
Sequence Type Exemplar sequence
Representative Public ID Y6E2A.4 NCBI
Target Description Y6E2A.4 /REP_DB=WormBase Gene ID /WP=CE16581 /TR=O45969 /GB=CAA15963.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074509(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074509 NCBI Caenorhabditis elegans Y6E2A.4 (Y6E2A.4) mRNA, complete cds. 11/11 A 
SNAP00000013590 ENSEMBL cdna:SNAP chromosome:CEL140:V:15714832:15718228:-1 11/11 None
GENEFINDER00000013604 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15715341:15718228:-1 11/11 None
Y6E2A.4 ENSEMBL cdna:known chromosome:CEL140:V:15714832:15718228:-1 gene:Y6E2A.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15716656-15718405(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y6E2A.4
Entrez Gene 189374 Entrez gene
SwissProt O45969 EMBL-EBI
WormBase CE31125 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA15963 Hypothetical protein Y6E2A.4 [Caenorhabditis elegans] ref|NP_506910.2| Y6E2A.4 [Caenorhabditis elegans] 0.0
blast CAA15963 Hypothetical protein Y6E2A.4 [Caenorhabditis elegans] ref|NP_506910.2| Y6E2A.4 [Caenorhabditis elegans] 1.0E-165
blast T27331 hypothetical protein Y6E2A.3 - Caenorhabditis elegans 7.0E-95
blast T27331 hypothetical protein Y6E2A.3 - Caenorhabditis elegans 2.0E-87
blast CAE66299 Hypothetical protein CBG11548 [Caenorhabditis briggsae] 4.0E-81
blast CAE66299 Hypothetical protein CBG11548 [Caenorhabditis briggsae] 5.0E-81
blast CAE66299 Hypothetical protein CBG11548 [Caenorhabditis briggsae] 6.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR007883 EMBL-EBI Protein of unknown function DUF713 9.9E-12
Pfam IPR007883 EMBL-EBI Protein of unknown function DUF713 4.0E-12
Sequence
Target Sequence 
>C. ELEGANS:179452_AT
tcatcgcgaatcccagaaaattatctgcaaggctgagaaagccaaaagacgatcaaccag
aagagttcttggattttgaaaatgatttgagagatgctcagaggaactggggagagtttt
tcagaaagtatgagaacgaaattaatatgtttacgggagcagcgaaacaatatggaaaag
tagatttccacgaggaattgtggcagcagacaaggtttcagaaactaacttcagccatca
caaacttttccgaaaagattcaaaacaaaaccgtatcctacatccctccatcacgatttt
tattcgcatttgttggttttcttgccagttggacaatagttccagtttttaatactccga
aaaagatttcttcaaaaatattcaccccgaagcatgttgagcagtcgatgaatggaaatg
gcgttcacaaaatggagaaaaatggaacaaatcacacaacaaaaaaccctattccatcga
aaatcattgttacgccacaggagaacgatgaagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATCGCGAATCCCAGAAAATTATC 222 621 341 Antisense
GTGGCAGCAGACAAGGTTTCAGAAA 643 491 540 Antisense
CTTCAGCCATCACAAACTTTTCCGA 224 221 569 Antisense
TCAAAACAAAACCGTATCCTACATC 511 627 600 Antisense
ATCCCTCCATCACGATTTTTATTCG 438 37 622 Antisense
TTTATTCGCATTTGTTGGTTTTCTT 353 675 639 Antisense
GTTGGTTTTCTTGCCAGTTGGACAA 151 415 652 Antisense
TATTCACCCCGAAGCATGTTGAGCA 422 663 719 Antisense
CACAACAAAAAACCCTATTCCATCG 707 191 795 Antisense
CCATCGAAAATCATTGTTACGCCAC 480 269 814 Antisense
TACGCCACAGGAGAACGATGAAGCT 490 639 831 Antisense
Sequence Source Affymetrix Proprietary Database